Export file:


  • RIS(for EndNote,Reference Manager,ProCite)
  • BibTex
  • Text


  • Citation Only
  • Citation and Abstract

Effective elimination of water-borne Escherichia coli using archaeal poly-g-glutamate-based materials

1 Course of Applied Bioresource Science, United Graduate School of Agricultural Sciences, Ehime University, Matsuyama, Ehime 790-8566, Japan
2 Faculty of Agriculture, Kochi University, Nankoku, Kochi 783-8502, Japan

Escherichia coli is used worldwide as a fecal indicator species to assess the quality of (drinking) water. Active carbons are used for the removal of chemical pollutants, but are ineffective in the inactivation of water-borne pathogens such as E. coli. Herein, we developed poly-g-glutamate-ion complex-coated active carbons (PGAIC-AC) and examined their ability to eliminate E. coli from a laboratory model of water pollution (~ 2.0 × 104 CFU/mL) at room temperature. The results showed that E. coli was virtually eliminated when using PGAIC-AC as a dispersant. In fact, the log reduction values were estimated to be > 1.19. In this study, we further constructed simple but effective bacteria-elimination system with a PGAIC-AC–embedded column. This PGAIC-AC system can be utilized to purify water when no electricity or specialized equipment is available.
  Article Metrics


1. Ashbolt NJ (2004) Microbial contamination of drinking water and disease outcomes in developing regions. Toxicology 198: 229–238.    

2. World Health Organization, The top 10 causes of death. World Health Organization, 2014. Available from: http://www.who.int/mediacentre/factsheets/fs310/en/.

3. Ministry of Health, Labour and Welfare, Food poisoning statistics document. Ministry of Health, Labour and Welfare, 2005–2014. Available from: http://www.mhlw.go.jp/stf/seisakunitsuite/bunya/kenkou_iryou/shokuhin/syokuchu/04.html.

4. Hu J, Aarts A, Shang R, et al. (2016) Integrating powdered activated carbon into wastewater tertiary filter for micro-pollutant removal. J Environ Manage 177: 45–52.

5. Ashiuchi M (2013) Microbial production and chemical transformation of poly-γ-glutamate. Microb Biotechnol 6: 664–674.

6. Ashiuchi M, Fukushima K, Oya H, et al. (2013) Development of antimicrobial thermoplastic material from archaeal poly-g-L-glutamate and its nanofabrication. ACS Appl Mater Interfaces 5: 1619–1624.    

7. Ashiuchi M, Hakumai Y, Shibatani S, et al. (2015) Poly-g-glutamate-based materials for multiple infection prophylaxis possessing versatile coating performance. Int J Mol Sci 16: 24588–24599.

8. Alm EW, Burke J, Hagan E (2006) Persistence and potential growth of the fecal indicator bacteria, Escherichia coli, in shoreline sand at Lake Huron. J Gt Lakes Res 32: 401–405.

9. Evans TM, Waarvuck CE, Seidler RJ, et al. (1981) Failure of the most-probable-number technique to detect coliforms in drinking water and raw water supplies. Appl Environ Microbial 41: 130–138.

10. Suhalima R, Huanga YW, Burtle GJ (2008) Survival of Escherichia coli O157:H7 in channel catfish pond and holding tank water. LWT-Food Sci Technol 41: 1116–1120.

11. Kasımoğlu A, Akgün S (2004) Survival of Escherichia coli O157:H7 in the processing and post-processing stages of acidophilus yogurt. Int J Food Sci Technol 39: 563–568.

12. Hakumai Y, Oike S, Shibata Y, et al. (2016) Cooperative adsorption of critical metal ions using archaeal poly-γ-glutamate. BioMetals 29: 527–534.

13. Ghaedi M, Ghaedi AM, Negintaji E, et al. (2014) Random forest model for removal of bromophenol blue using activated carbon obtained from Astragalus bisulcatus tree. J Ind Eng Chem 20: 1793–1083.

14. Sampranpiboon P, Charnkeitkong P, Feng X (2014) Equilibrium isotherm models for adsorption of zinc (II) ion from aqueous solution on pulp waste. WSEAS Trans Environ Dev 10: 35–47.

15. Langmuir I (1916) The adsorption of gases on plane surface of glass, mica and platinum. J Am Chem Soc 40: 1361–1403.

16. World Health Organization and United Nations Children's Fund (2006) Meeting the MDG drinking-water and sanitation target: The urban and rural challenge of the decade. World Health Organization and United Nations Children's Fund. Available from: http://www.who.int/water_sanitation _health/monitoring/jmp2006/en/.

17. United Nations Population Fund (2006) State of World Population 2006. United Nations Population Fund. Available from: http://www.unfpa.org/publications/state-world-population-2006.

18. World Health Organization (2004) Water, sanitation and hygiene links to health. World Health Organization. Available from: http://www.who.int/water_sanitation_health/facts2004/en/.

19. Udomchoke V, Sunthornranun P, Apisit Songsasen A, et al. (2010) The ecological complexity of the Thai-Laos Mekong River: I. Geology, seasonal variation and human impact assessment on river quality. J Environ Sci Health A: Toxic/Hazard Subst Environ Eng 45: 1661–1673.

Copyright Info: © 2016, Makoto Ashiuchi, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

Download full text in PDF

Export Citation

Article outline

Show full outline
Copyright © AIMS Press All Rights Reserved