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The prevalence of foodborne pathogenic bacteria on cutting boards and their ecological correlation with background biota

1 Department of Biological Functions and Engineering, Graduate School of Life Science and Systems Engineering, Kyushu Institute of Technology, 2-4 Hibikino, Wakamatsu-ku, Kitakyushu, Fukuoka 808-0196, Japan
2 Department of Medical Microbiology and Parasitology, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
3 Department of Food Service and Management, Faculty of Food Science and Technology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
4 Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
5 Laboratory of Soil Microbiology, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan
6 Laboratory of Molecular Gene Technique, Faculty of Agriculture, Graduate School, Kyushu University, 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581, Japan

Topical Section: Pathogenic microorganisms and human health

This study implemented the pyrosequencing technique and real-time quantitative PCR to determine the prevalence of foodborne pathogenic bacteria (FPB) and as well as the ecological correlations of background biota and FPB present on restaurant cutting boards (CBs) collected in Seri Kembangan, Malaysia. The prevalence of FPB in high background biota (HBB) was lower (0.24%) compared to that of low background biota (LBB) (0.54%). In addition, a multiple linear regression analysis indicated that only HBB had a significant ecological correlation with FPB. Furthermore, statistical analysis revealed that the combinations of Clostridiales, Flavobacteriales, and Lactobacillales orders in HBB had significant negative associations with FPB, suggesting that these bacteria may interact to ensure survivability and impair the growth of pathogenic bacteria.
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Keywords microbial diversity; foodborne pathogenic bacteria; pyrosequencing analysis; bacterial orders; background biota

Citation: Noor-Azira Abdul-Mutalib, Syafinaz Amin Nordin, Malina Osman, Ahmad Muhaimin Roslan, Natsumi Ishida, Kenji Sakai, Yukihiro Tashiro, Kosuke Tashiro, Toshinari Maeda, Yoshihito Shirai. The prevalence of foodborne pathogenic bacteria on cutting boards and their ecological correlation with background biota. AIMS Microbiology, 2016, 2(2): 138-151. doi: 10.3934/microbiol.2016.2.138


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