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Gelatin methacrylate-alginate hydrogel with tunable viscoelastic properties

1 Essen Bioscience Inc. 300 W Morgan Rd, Ann Arbor, MI 48108 USA
2 Draper Laboratory: 555 Technology Square, Cambridge, MA 02139 USA
3 Department of Biomedical and Chemical Engineering, Syracuse University, 900 S Crouse Ave, Syracuse NY 13210 USA

Topical Section: Biological and biomimetic materials

Although native extracellular matrix (ECM) is viscoelastic, synthetic biomaterials used in biomedical engineering to mimic ECM typically exhibit a purely elastic response when an external strain is applied. In an effort to truly understand how living cells interact with surrounding ECM matrix, new biomaterials with tunable viscoelastic properties continue to be developed. Here we report the synthesis and mechanical characterization of a gelatin methacrylate-alginate (Gel-Alg) composite hydrogel. Results obtained from creep and compressive tests reveal that the alginate component of Gel-Alg composite, can be effectively crosslinked, un-crosslinked and re-crosslinked by adding or chelating Ca2+ ions. This work demonstrates that Gel-Alg is capable of tuning its viscoelastic strain and elastic recovery properties, and can be potentially used to design ECM-mimicking hydrogels.
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Keywords gelatin; alginate; hydrogel; viscoelastic properties; elastic recovery

Citation: Yong X. Chen, Brian Cain, Pranav Soman. Gelatin methacrylate-alginate hydrogel with tunable viscoelastic properties. AIMS Materials Science, 2017, 4(2): 363-369. doi: 10.3934/matersci.2017.2.363

References

  • 1. Tayalia P, Mendonca CR, Baldacchini T, et al. (2008) 3D Cell-Migration Studies using Two-Photon Engineered Polymer Scaffolds. Adv Mater 20: 4494–4498.    
  • 2. Annabi N, Tamayol A, Uquillas JA, et al. (2014) 25th anniversary article: rational design and applications of hydrogels in regenerative medicine. Adv Mater 26: 85–124.    
  • 3. Lee KY, Mooney DJ (2001) Hydrogels for tissue engineering. Chem Rev 101: 1869–1880.    
  • 4. Tibbitt MW, Anseth KS (2009) Hydrogels as extracellular matrix mimics for 3D cell culture. Biotechnol Bioeng 103: 655–663.    
  • 5. Storm C, Pastore JJ, MacKintosh FC, et al. (2005) Nonlinear elasticity in biological gels. Nature 435: 191–194.    
  • 6. Wen Q, Janmey PA (2013) Effects of nonlinearity on cell-ECM interactions. Exp Cell Res 319: 2481–2489.    
  • 7. Burdick JA, Murphy WL (2012) Moving from static to dynamic complexity in hydrogel design. Nat Commun 3: 1269.    
  • 8. McKinnon DD, Domaille DW, Cha JN, et al. (2014) Biophysically defined and cytocompatible covalently adaptable networks as viscoelastic 3D cell culture systems. Adv Mater 26: 865–872.    
  • 9. Hong X, Stegemann JP, Deng CX (2016) Microscale characterization of the viscoelastic properties of hydrogel biomaterials using dual-mode ultrasound elastography. Biomaterials 88: 12–24.    
  • 10. Wang H, Heilshorn SC (2015) Adaptable hydrogel networks with reversible linkages for tissue engineering. Adv Mater 27: 3717–3736.    
  • 11. Sun TL, Kurokawa T, Kuroda S, et al. (2013) Physical hydrogels composed of polyampholytes demonstrate high toughness and viscoelasticity. Nat Mater 12: 932–937.
  • 12. Rodell CB, MacArthur JW, Dorsey SM, et al. (2015) Shear-Thinning Supramolecular Hydrogels with Secondary Autonomous Covalent Crosslinking to Modulate Viscoelastic Properties In Vivo. Adv Funct Mater 25: 636–644.    
  • 13. Chaudhuri O, Gu L, Klumpers D, et al. (2016) Hydrogels with tunable stress relaxation regulate stem cell fate and activity. Nat Mater 15: 326–334.
  • 14. Gillette BM, Jensen JA, Wang M, et al. (2010) Dynamic Hydrogels: Switching of 3D Microenvironments Using Two-Component Naturally Derived Extracellular Matrices. Adv Mater 22: 686–691.    
  • 15. Park H, Kang SW, Kim BS, et al. (2009) Shear-reversibly crosslinked alginate hydrogels for tissue engineering. Macromol Biosci 9: 895–901.    
  • 16. Stowers RS, Allen SC, Suggs LJ (2015) Dynamic phototuning of 3D hydrogel stiffness. Proceedings of the National Academy of Sciences, 112: 1953–1958.
  • 17. Gonen-Wadmany M, Oss-Ronen L, Seliktar D (2007) Protein-polymer conjugates for forming photopolymerizable biomimetic hydrogels for tissue engineering. Biomaterials 28: 3876–3886.    
  • 18. Nichol JW, Koshy ST, Bae H, et al. (2010) Cell-laden microengineered gelatin methacrylate hydrogels. Biomaterials 31: 5536–5544.    
  • 19. Soman P, Chung PH, Zhang AP, et al. (2013) Digital microfabrication of user-defined 3D microstructures in cell-laden hydrogels. Biotechnol Bioeng 110: 3038–3047.    
  • 20. Fairbanks BD, Singh SP, Bowman CN, et al. (2011) Photodegradable, photoadaptable hydrogels via radical-mediated disulfide fragmentation reaction. Macromolecules 44: 2444–2450.    
  • 21. Chen YX, Yang S, Yan J, et al. (2015) A Novel Suspended Hydrogel Membrane Platform for Cell Culture. J Nanotechnol Eng Med 6: 021002.    

 

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Copyright Info: 2017, Yong X. Chen, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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