Research article

Molecular characterization of the netrin-1 UNC-5 receptor in Lucilia sericata larvae

  • Larval therapy with Lucilia sericata is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. Netrin-1, one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The UNC-5 receptor combines with a netrin-1 signal and transmits the signal from one side of the membrane to the other side, initiating a change in cell activity. In the current study, we identified the full length of the UNC-5 receptor mRNA in L. sericata using different sets of primers, including exon junction and specific region primers. The coding sequence (CDS) of the UNC-5 receptor was sequenced and identified to include 633 base-pair nucleic acids, and BLAST analysis on its nucleotide sequence revealed 96% identity with the Lucilia cuprina netrin-1 UNC-5 receptor. The protein residue included 210 amino acids (aa) and coded for a protein with 24 kD weight. This gene lacked the signal peptide. Furthermore, the UPA domain is conserved in UNC-5. It lied at the interval of 26–131 aa. We identified the CDS of netrin-1 UNC-5 receptor in L. sericata. It could be applied to research activities implementing a new essential component design in wound healing.

    Citation: Tahereh Karamzadeh, Hamzeh Alipour, Marziae Shahriari-Namadi, Abbasali Raz, Kourosh Azizi, Masoumeh Bagheri, Mohammad D. Moemenbellah-Fard. Molecular characterization of the netrin-1 UNC-5 receptor in Lucilia sericata larvae[J]. AIMS Genetics, 2019, 6(3): 46-54. doi: 10.3934/genet.2019.3.46

    Related Papers:

    [1] Julien G. Mahy, Stéphanie D. Lambert, Jérémy Geens, Alain Daniel, David Wicky, Catherine Archambeau, Benoît Heinrichs . Large scale production of photocatalytic TiO2 coating for volatile organic compound (VOC) air remediation. AIMS Materials Science, 2018, 5(5): 945-956. doi: 10.3934/matersci.2018.5.945
    [2] Khang Duy Vu Nguyen, Khoa Dang Nguyen Vo . Magnetite nanoparticles-TiO2 nanoparticles-graphene oxide nanocomposite: Synthesis, characterization and photocatalytic degradation for Rhodamine-B dye. AIMS Materials Science, 2020, 7(3): 288-301. doi: 10.3934/matersci.2020.3.288
    [3] Fahmi Astuti, Rima Feisy Azmi, Mohammad Arrafi Azhar, Fani Rahayu Hidayah Rayanisaputri, Muhammad Redo Ramadhan, Malik Anjelh Baqiya, Darminto . Employing Na2CO3 and NaCl as sources of sodium in NaFePO4 cathode: A comparative study on structure and electrochemical properties. AIMS Materials Science, 2024, 11(1): 102-113. doi: 10.3934/matersci.2024006
    [4] Xuan Luc Le, Nguyen Dang Phu, Nguyen Xuan Duong . Enhancement of ferroelectricity in perovskite BaTiO3 epitaxial thin films by sulfurization. AIMS Materials Science, 2024, 11(4): 802-814. doi: 10.3934/matersci.2024039
    [5] Liang Wu . Cu-based mutlinary sulfide nanomaterials for photocatalytic applications. AIMS Materials Science, 2023, 10(5): 909-933. doi: 10.3934/matersci.2023049
    [6] Evangelos Karagiannis, Dimitra Papadaki, Margarita N. Assimakopoulos . Circular self-cleaning building materials and fabrics using dual doped TiO2 nanomaterials. AIMS Materials Science, 2022, 9(4): 534-553. doi: 10.3934/matersci.2022032
    [7] Jie Sun, Tzvetanka Boiadjieva-Scherzer, Hermann Kronberger, Kevin Staats, Johannes Holinka, Reinhard Windhager . Surface modification of Ti6Al4V alloy for implants by anodization and electrodeposition. AIMS Materials Science, 2019, 6(5): 713-729. doi: 10.3934/matersci.2019.5.713
    [8] Yana Fajar Prakasa, Sumari Sumari, Aman Santoso, Muhammad Roy Asrori, Ririn Cahyanti . The performance of radar absorption of MnxFe3–xO4/rGO nanocomposites prepared from iron sand beach and coconut shell waste. AIMS Materials Science, 2023, 10(2): 227-248. doi: 10.3934/matersci.2023013
    [9] Raghad Subhi Abbas Al-Khafaji, Kareem Ali Jasim . Dependence the microstructure specifications of earth metal lanthanum La substituted Bi2Ba2CaCu2-XLaXO8+δ on cation vacancies. AIMS Materials Science, 2021, 8(4): 550-559. doi: 10.3934/matersci.2021034
    [10] Kah Hon Leong, Azrina Abd Aziz, Yee Li Kang, Sheau Wei Goh, Kulhar Vijay Singh, Lan Ching Sim, Pichiah Saravanan . Synergized mechanistic and solar photocatalysis features of N-TiO2 functionalised activated carbon. AIMS Materials Science, 2017, 4(3): 800-813. doi: 10.3934/matersci.2017.3.800
  • Larval therapy with Lucilia sericata is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. Netrin-1, one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The UNC-5 receptor combines with a netrin-1 signal and transmits the signal from one side of the membrane to the other side, initiating a change in cell activity. In the current study, we identified the full length of the UNC-5 receptor mRNA in L. sericata using different sets of primers, including exon junction and specific region primers. The coding sequence (CDS) of the UNC-5 receptor was sequenced and identified to include 633 base-pair nucleic acids, and BLAST analysis on its nucleotide sequence revealed 96% identity with the Lucilia cuprina netrin-1 UNC-5 receptor. The protein residue included 210 amino acids (aa) and coded for a protein with 24 kD weight. This gene lacked the signal peptide. Furthermore, the UPA domain is conserved in UNC-5. It lied at the interval of 26–131 aa. We identified the CDS of netrin-1 UNC-5 receptor in L. sericata. It could be applied to research activities implementing a new essential component design in wound healing.


    In the past decades, in the face of water pollution caused by organic dyes from various industrial plants such as paper, printing ink, fabic and industrial dyeing, etc., the search for solutions to improve the water environment is the main concern of researchers. With the achievements of science and technology, water pollution treatment through advanced oxidation process AOP using semiconductor photocatalysts was born and developed strongly [1,2,3]. Photocatalysis is a method that exhibits outstanding advantages compared to conventional methods such as high efficiency, low cost, environmental friendliness. In addition, it can also take advantage of the infinite excitation energy from the Sun. Since Fujishima and Honda first discovered the photocatalytic ability of TiO2 electrode in 1972 [4], metal oxide-based photocatalysts such as TiO2, ZnO, SnO2, ABO4, A2B2O7, etc. have been studied extensively and shown high photocatalytic efficiency. However, a major drawback of these semiconductors is the large energy band gap (>3 eV), which can only use ultraviolet light, a small fraction of solar radiation, to stimulate electron-hole generation. A large part of research effort on these materials is focused on modifying materials to have smaller band gap such as doping [5,6,7], compositing [8,9,10,11] and loading [12,13]. At the same time, the search and development of new photocatalysts that work efficiently under visible light has always been a big goal in recent years.

    Among the new materials discovered in the last decade, Ag3PO4 (APO) is considered as one of the bright candidates for visible light photocatalyst [14]. It is proven that APO has a narrow band gap energy of 2.43 eV and an excellent photocatalytic activity with a quantum yield of 90% [15] with the ability to decompose a wide range of organic substances such as rhodamine B [16], bisphenol A [17], phenol [18,19], methylene blue [20], quinoline yellow [21], methyl orange [22], and microcystins [23], etc. Studies show that the morphology and particle size of APO greatly affect the photocatalytic performance of the material. In turn, APO particle morphology is influenced by several factors such as the method of synthesis (co-precipitation, hydrothermal, microwave-assisted chemistry, etc.), phosphate ion source (basic phosphate salts or phosphoric acid), the pH of the starting solution and surfactant. For example, APO particles with very different pseudospherical particle morphology and size were prepared from basic phosphate salts Na3PO4, Na2HPO4, NaH2PO4 by co-precipitation method in which the sample prepared from Na2HPO4 exhibited the highest photocatalytic efficiency to degrade methylene blue [24]. Meanwhile, tetrahedral APO particles were produced using phosphoric acid H3PO4 [24]. Visible light-responsive Ag3PO4 photocatalysts with fern-like, multipod-like and tetrapod-like morphologies were successfully synthesized by facile soft-chemical technique from phosphoric acid H3PO4 and AgNO3 in the presence of tetrahydrofuran (THF) surfactant with a THF/water volumetric ratio of 0:1, 0.11:1, 0.13:1 [25]. By decreasing pH through increasing ammonia NH4 concentration at 0.00 M, 0.05 M, 0.15 M and 0.30 M, APO particles of decreasing size with NH4 concentration are produced and accordingly photocatalytic ability increased [26]. Extremely small APO nanoparticles of about 10 nm with outstanding visible light photocatalysis were produced by simple ion-exchange method from (NH4)2HPO4 [27]. APO composed of several microcrystals with polydisperse size and shape were synthesized by microwave-assisted hydrothermal method using (NH4)2HPO4 as phosphate ion source [28]. Different concentrations of KH2PO4 initiator were also used to change the morphology and size of APO particles and thus control its photocatalytic ability [15]. In general, the studies used phosphate moiety as the precipitation agent in the form of salts or acids. However, to date, there have been no studies specifically comparing the effect of different phosphate moieties on the structure and physical and photocatalytic properties of APO.

    In this study, we would like to clarify the influence of phosphate sources as monobasic and dibasic salts on the morphology and photocatalytic properties of APO. The selected phosphate salts are of two metals potasium and sodium (K2HPO4, KH2PO4, Na2HPO4, NaH2PO4). The work focused on showing which phosphate source (monobasic or dibasic) is more suitable to produce APO with strong photocatalytic activity. In addition, the structural and other physical properties were also thoroughly analyzed to support the interpretation of the photocatalytic activity.

    Silver nitrate (AgNO3, Merck, 99%), potassium phosphate dibasic trihydrate (K2HPO4.3H2O, Merck, 99%), potassium phosphate monobasic (KH2PO4, Merck, 99%), sodium phosphate dibasic dodecahydrate (Na2HPO4·12H2O, Merck, 99%), and sodium phosphate monobasic dihydrate (NaH2PO4·2H2O, Merck, 99%) were used as starting chemicals. Rhodamine B (C28H31ClN2O3, Merck, 95%) was used as an organic colorant in the photocatalytic test. These starting materials were used without further purification. A Xenon lamp (300W/220V) with an ultraviolet filter were used as the visible light source irradiation.

    In this study, we synthesize Ag3PO4 photocatalyst by a simple precipitation method. An amount of AgNO3 was dissolved in a beaker containing 150 mL of distilled water to obtain an Ag+ 0.02 M solution. In another beaker, a suitable phosphate salt (K2HPO4, KH2PO4, Na2HPO4, NaH2PO4) is dissolved in an analysis amount of distilled water such that a 0.02 M solution of PO43– is obtained. The stoichiometric amount of phosphate salts was specified such that the Ag+/PO4 ratio is of 3:1.5 according to our previous investigation for optimization of synthesis condition. The solution containing Ag+ ions was then slowly added into the solution containing PO43– ions and magnetically stirred to obtain a homogeneous solution. This solution was continued to be magnetically stirred for the next 3 h at room temperature until a stable amount of yellow precipitate was formed. The precipitate was filtered and washed 5 times with distilled water before being dried at 100oC to obtain a yellow powder of Ag3PO4.

    Samples of APO prepared from different salts K2HPO4, KH2PO4, Na2HPO4, and NaH2PO4 were respectively named after the chemical formula of the starting salt as APOK2H, APOKH2, APONa2H, and APONaH2 for convenience in description.

    X-ray diffractometer (XRD, Brucker D8 Advance) using Cu Kα radiation (λ = 1.54064 Å) was used to determine the crystal structure of as-synthesized APO samples with Bragg angles ranging from 10º to 110º. The surface morphology of the samples was observed using a scanning electron microscope (SEM, JED-2300). The Brunauer-Emmett-Teller (BET) surface area was analyzed using a high-performance adsorption analyzer (Micromeritics 3Flex). An infrared spectrophotometer (Shimadzu IR Prestige-21) was used to measure the Fourier transform infrared (FTIR) absorption spectra. A Raman spectrophotometer (Horiba LabRam HR Evolution) using a 532 nm laser beam as the excitation source was used to measure Raman scattering spectra. UV-vis diffuse reflectance spectra (DRS) were performed on a UV-vis spectrophotometer (Jasco V670). Luminescence (PL) spectra were carried out on a fluorescence spectrometer (Nanolog Horiba iHR 550) using an excitation beam at 350 nm.

    In this study, visible-light photocatalytic activity of Ag3PO4 was evaluated based on the degradation of 10 ppm rhodamine B (RhB) solution. The visible light used for excitation is obtained from a Xenon lamp (300 W/220 V) using an ultraviolet filter.

    At the first step, 30 mL of 20 ppm RhB solution was placed in a 6 cm diameter beaker. In another beaker, 0.6 g of APO was dissolved in 30 ml of distilled water, sonicated for 30 min. Next, slowly add the 20 ppm RhB solution into the APO-contained beaker to obtain a mixture with RhB concentration of 10 ppm. The mixture was immediately magnetically stirred in a dark chamber for 30 min to reach adsorption-desorption equilibrium. After the first 10 min of dark stirring, 4 mL of the solution was removed to evaluate the adsorption capacity of the sample. After dark stirring for 30 min, the solution was illuminated under a Xenon lamp using a UV-cut filter. The distance between the Xenon lamp and the surface of the RhB solution is around 10 cm (~23000 lux illuminance). An amount of 4 mL of solution was removed every one min and centrifuged at 4000 rpm to remove the APO powder. Absorption spectra were used to evaluate the remaining RhB concentration in solution (using 554 nm characteristic absorption peak of RhB).

    Figure 1a shows the X-ray diffraction patterns of as-synthesized Ag3PO4 (APO) photocatalysts with different starting phosphate salts (K2HPO4, KH2PO4, Na2HPO4, NaH2PO4). All the patterns match well with the body-centered cubic Ag3PO4 according to JCPDS card No. 06-0505 without any strange reflexes of the impurity phases. A comparison of the (210) plane position was carried out (Figure 1b) that indicates a given shift to the larger 2-theta angle when using monobasic phosphate salts (APOKH2, APONaH2), which should theoretically lead to a smaller lattice parameter $ a $. Table 1 presents calculated lattice constant $ a $ as well as unit cell volume $ V $ in angstrom unit, in which the lattice constants $ a $ is 5.962 Å, 5.945 Å, 5.965 Å, and 5.942 Å for the APOK2H, APOKH2, APONa2H, and APONaH2 samples, respectively. Obviously, the crystals prepared from the monobasic phosphate salts possess the smaller lattice parameter than that of the samples synthesized from dibasic salt. A comparation of the intensity of the line (210) showed greater intensity for the samples fabricated from the monobasic phosphate salts where the ratios of IAPOKH2/IAPOK2H and IAPONaH2/IAPONa2H are 1.05 and 1.50, respectively. In addition, the crystallinity is also evaluated by the crystallite size $ D $XRD that can be inferred from the full width at half maximum $ FWHM $ of diffraction lines using the Debye-Scherrer equation. Figure 1b shows the narrower of the (210) line for the samples APOKH2 and APONaH2, leading to the calculated results for a larger $ D $XRD as shown in Table 1. The crystallite size is 41 nm, 48 nm, 33 nm, and 48 nm for the APOK2H, APOKH2, APONa2H, and APONaH2 samples, respectively. The expression of the small lattice parameter and the large crystal size shows that the samples APOKH2 and APONaH2 samples crystallize better than those synthesized from dibasic phosphate salts. This may be a manifestation of the influence of the pH on the crystallization of APO, the lower the pH, the greater the crystallinity. The strong crystallization of APO synthesized from monobasic phosphate salts could be explained by the competition between OH and PO43– that reacts with Ag on the surface of Ag3PO4 nuclei during the growth proccess [29]. As well known, KH2PO4 and NaH2PO4 are acidic salts (pH ~ 5.7) while K2HPO4 and Na2HPO4 are basic salts (pH ~ 7.5). In the solvent of monobassic phosphate salt, the amount of OH ions on the surface of the Ag3PO4 nucleus is less than in the solvent of dibasic phosphate salts, so PO43– easily combines with Ag+ leading to better growth of APOKH2 and APONaH2 crystals.

    Figure 1.  (a) XRD patterns of Ag3PO4 photocatalysts synthesized from different phosphate salts (K2HPO4, KH2PO4, Na2HPO4, NaH2PO4) and (b) a comparison of (201) reflexes position in different samples.
    Table 1.  Some parameters of Ag3PO4 crystals synthesized from different phosphate salts (K2HPO4, KH2PO4, Na2HPO4, NaH2PO4) such as lattice constant $ a $, unit cell volume $ V $, full width of half maximum $ FWHM $, crystal size $ D $XRD and particle size $ D $SEM.
    Samples APOK2H APOKH2 APONa2H APONaH2
    $ a=b=c $ Å 5.962 5.945 5.965 5.942
    $ V\left({\rm{Å}}^{3}\right) $ 212.022 210.128 212.207 209.830
    $ FWHM $ (o) 0.214 0.185 0.272 0.182
    Crystallite size $ D $XRD (nm) 41 48 32 48
    Particle size $ D $SEM (μm) 0.8 3.2 0.4 1.6

     | Show Table
    DownLoad: CSV

    The surface morphology of as-synthesized AOP particles was investigated by the SEM measurements (Figure 2). Particle size distributions were performed through ImageJ software and is represented in Figure 3. Figure 2a, c represent the external morphology of the APOK2H and APONa2H samples, consisting of pseudospherical particles of averaging diameter around of 800 nm and 400 nm (Figure 3a, c), respectively, with occasional inclusions of a few particles with geometric polygon faces (dotted circle). This result complements the inference that the OH group inhibits the reaction of PO43– and Ag+ to form Ag3PO4 in basic solvents. Therefore, not only the crystal grain size is small, but there is also no preferred crystal growth orientation. Meanwhile, the SEM images of the two samples APOKH2 and APONaH2 (Figure 2b, d) show much larger particles, averaging around 3.2 μm and 2.6 μm, respectively. In addition, most particles have a polygonal shape with distinct geometrical facets. The sample APOKH2 contains mainly dodecahedral particles while the sample APONaH2 contains cubic ones. In the APOKH2 sample, a variety of grain morphology can also be found such as tetrapot (ⅰ), pyramid (ⅱ), and triangular plate (ⅲ) (inset figure in Figure 2b). Therefore, it can be said that low pH conditions not only facilitated stronger growth of APO crystallites but also promote anisotropic crystallization along certain prority axes to produce crystals with diverse morphologies and sizes many times larger than those of APO prepared from high pH solvent [15,29,30]. In addition, correlation between crystal size DXRD and particle size DSEM (Table 1) also shows that low pH conditions both promote crystallization and agglomeration of single crystals into large particles.

    Figure 2.  SEM images of Ag3PO4 photocatalysts synthesized from different phosphate salts (a) K2HPO4, (b) KH2PO4, (c) Na2HPO4, and (d) NaH2PO4.
    Figure 3.  Particle size distribution of Ag3PO4 photocatalysts synthesized from different phosphate salts (a) K2HPO4, (b) KH2PO4, (c) Na2HPO4, and (d) NaH2PO4.

    Figure 4a shows the FTIR absorption spectra of as-synthesized APO samples which indicates some sharp absorbance centered at 546 cm−1, 1018 cm−1, 1385 cm−1, 1661 cm−1, 2364 cm−1, and 3234 cm−1. The peaks at 546 cm−1 and 1018 cm−1 are attributed to the characteristic vibrations of the [PO4] cluster in crystal lattice, corresponding to $ \nu 4 $ antisymmetric bending mode and $ \nu 3 $ antisymmetric stretching mode [31,32]. Two peaks at 1661 cm−1 and 3234 cm−1 could be attributed to the oscillations of water molecules adsorbed on the APO surface while the adsorbed CO2 molecules exhibited vibration peak at the 2364 cm−1 [33]. Alternatively, the band at 1390 cm−1 can be specified for nitrate groups generated from synthetic residues. To indirectly observe the change in APO lattice structure, a detailed comparison of the FTIR absorption between 400–1200 cm−1 was carried out and presented in Figure 4b. The results show that while the three APOK2H, APONa2H, and APOKH2 samples exhibit relatively similar FTIR absorption peaks of the [PO4] group at 546 cm−1, 1004 cm−1 and 1037 cm−1, the APONaH2 sample shows a shift of peaks towards a larger frequency, which is consistent with the smallest lattice constant of APONaH2 as observed in the XRD results.

    Figure 4.  (a) FTIR spectra of Ag3PO4 photocatalysts synthesized from different phosphate salts (K2HPO4, KH2PO4, Na2HPO4, NaH2PO4) and (b) a position comparison of some FTIR peaks in the range of 400–1200 cm−1.

    Another useful tool for indirectly observing crystal structure and their small changes is the Raman scattering spectra (Figure 5). Figure 5a shows that in the range of wave number 200–1200 cm−1, APO exhibits seven vibrational peaks centered at wave numbers of 151 cm−1, 281 cm−1, 406 cm−1, 577 cm−1, 910 cm−1, 956 cm−1, and 1057 cm−1, all of them are characteristic vibrations of [PO4] cluster. The intensive peak at 910 cm−1 could be assigned to the symmetric stretching mode (A1) of [PO4] group while the asymmetric stretching vibration (T2) of this group are at 956 cm−1 and 1057 cm−1. The peaks at 406 cm−1 and 577 cm−1 were the symmetric (E) and asymmetric (T2) bending modes while the peaks at 151 cm−1 and 281 cm−1 were attributed to rotation or translation mode [34] of [PO4] unit. Figure 5b details the positions of several Raman peaks in the range of wave number 180–650 cm−1, which shows a strong Raman shift in different samples. The two samples synthesized from dibasic salts exhibited vibration peaks at low wave numbers of about 239 cm−1 and 557 cm−1 while these peaks are at 282 cm−1 and 582 cm−1 for the APOKH2 and APONaH2 samples. Theoretically, the vibration frequency is determined by the atomic mass and the interatomic spacing, thus the vibration frequency increase of [PO4] cluster in APOKH2 and APONaH2 samples indirectly confirms the decrease of the lattice parameter, consistent well with XRD and FTIR results.

    Figure 5.  (a) Raman scattering spectra of Ag3PO4 photocatalysts synthesized from different phosphate salts (K2HPO4, KH2PO4, Na2HPO4, NaH2PO4) and (b) a position comparison of some Raman peaks in the range of 150–650 cm−1.

    Figure 6 shows UV-vis absorption spectra of APO synthesized from different phosphate salts (K2HPO4, KH2PO4, Na2HPO4, and NaH2PO4). All samples exhibit an absorption edge at around 530 nm, thus suitable for using part of visible light band for photocatalytic excitation. The absorption edge for two samples APOKH2 and APONaH2 shifts slightly to the larger wavelength comparing to that of APOK2H and APONa2H. The bandgap of the photocatalyst was determined by the Wood-Tauc plot method where $ \left(\alpha h\nu \right) $2 is graphed as a function of photon energy as for direct semiconductor (Figure 6b). The identified values of Eg are around of 2.38 eV and 2.42 eV for APOKH2/APONaH2 and APOK2H/APONa2H, respectively, which is in good agreement with previous studies [35,36]. This result shows that the samples APOKH2 and APONaH2 can even absorb a wider range of radiation in the visible range than the samples APOK2H and APONa2H. The correlation between band gap energy and crystal size is appropriately explained by the quantum size effect [37].

    Figure 6.  (a) UV-vis absorption spectra of as-synthesized Ag3PO4 with different starting phosphate salts and (b) the method to determine energy band gap from the plot of $ {\left(\alpha h\nu \right)}^{2} $ versus photon energy $ (hc/\lambda) $.

    The photoluminescence PL spectra, which indirectly inform the recombination rate of photogenerated electron-hole, are shown in Figure 7. The result indicates that all samples emit irradiation over a wide range from 400 to 600 nm with a maximum between 500 to 530 nm and another at around 420 nm. PL properties of APO was attributed to the existence of [PO4] and [AgO4] clusters in which the blue PL emission is assumed to be caused by tetragonal [PO4] unit while the distorted tetragonal [AgO4] responds to the red PL emission [28,38]. Figure 7 shows that the different samples have slightly different PL intensities, where the APOK2H sample exhibits the lowest PL intensity, which can infer the smallest electron-hole recombination rate.

    Figure 7.  PL spectra of as-synthesized Ag3PO4 with different initial phosphate salts.

    The photocatalytic properties of as-prepared APO were evaluated through the decomposition of RhB solution under the visible light irradiation. The remaining RhB concentrations at each time were assessed through the intensity of RhB characteristic absorption peak at 554 nm. Figure 8 shows the results of the photocatalytic test of the as-synthesized APO. During the first 30 min, the RhB solution was stirred in the dark to reach adsorption-desorption equilibrium. Figure 8a indicates that after only 10 min an equilibrium state was established. In addition, the adsorption capacity of APO photocatalysts is also very poor, only around 5%. This can be explained by the large particle size of the material (several μm) as observed. When illuminated, all samples showed high photocatalytic activity where APOK2H and APONa2H completely degraded RhB in just 8 min and 10 min while samples APOKH2 and APONaH2 required 20 and 25 min, respectively.

    Figure 8.  (a) Photocatalytic activities and (b) reaction rate of as-synthesized Ag3PO4 in decomposing RhB solution under visible light using Xenon lamp.

    The photocatalytic reaction rate $ k $ was evaluated using the pseudo-first-order kinetic model, $ ln({C}_{o}/C) = kt $, where the reaction rate $ k $ could be obtained from the slope of the linear relationship of the plot $ ln({C}_{o}/C) $ versus reaction time (Figure 8b). The obtained values of $ k $ were 0.621, 0.444, 0.243, and 0.123 for APOK2H, APONa2H, APOKH2 and APONaH2, respectively. It is obvious that the visible light photocatalytic performance of APO samples synthesized from dibasic phosphate salts, which have poorer crystallinity and wider band gap, is better than that of the samples prepared from monobase salts. This can be attributed to the significant contribution of small particle size and therefore large specific surface area to the photocatalytic activity. However, APO prepared from potasium phosphate salts (monobasic or dibasic) is more active compared to APO synthesized from the salt of sodium, despite the larger particle size. This proves that in terms of selectivity, the phosphate salt of potassium is more suitable for the production of high photocatalytically active APO. The superior photocatalytic activity of APO is explained by the narrow bandgap energy. APO can effectively excited by visible light to produce electron-hole pairs that are directly involved in the oxidation of organic pollutants [39,40].

    Dibasic and monobasic phosphate salts of potassium and sodium used as precipitants have a strong influence on the crystallinity, crystal size, optical properties and even the photocatalytic activity of Ag3PO4. When using dibasic phosphate salt, APO crystallizes slowly, leading to small particle size, low electron/hole recombination rate, and superior photocatalytic activity. In addition, APO also exhibits higher photocatalytic activity when prepared from phosphate salts of potassium metal.

    This research was funded by a scientific and technological project at the level of Ministry of Education and Training, grand number B2020-MDA-11.

    The authors declare that they have no conflict of interest.

    Methodology and experiment, Mai Vu Thanh, Hang Lam Thi, Duyen Pham Thi, Dao La Bich, Anh Nguyen Thi Dieu; formal analysis, Hang Lam Thi, Duyen Pham Thi; investigation, Chung Pham Do, Hung Nguyen Manh; writing-original draft preparation, Oanh Le Thi Mai; writing-review and editing, Hung Nguyen Manh, Oanh Le Thi Mai, Chung Pahm Do; supervision, Minh Nguyen Van. All authors have read and agreed to the published version of the manuscript.


    Acknowledgments



    We appreciate colleagues in the Department of Medical Entomology, School of Health, for their collaboration in this research activity. This project was funded by the Shiraz University of Medical Sciences (SUMS), Shiraz, Iran [Grant #: 1396-01-04-15875] allocated to H.A. It was part of an MSc thesis in Medical Entomology and Vector Control solicited to the student, Ms. T. Karamzadeh.

    [1] Malekian A, Esmaeeli Djavid Gh, Akbarzadeh K, et al. (2019) Efficacy of maggot therapy on staphylococcus aureus and pseudomonas aeruginosa in diabetic foot ulcers: A randomized controlled trial. J Wound Ostomy Continence Nurs 46: 25–29. doi: 10.1097/WON.0000000000000496
    [2] Iorio V, Troughton LD, Hamill KJ (2015) Laminins: Roles and utility in wound repair. Adv Wound Care 4: 250–263. doi: 10.1089/wound.2014.0533
    [3] Mitchell KJ, Doyle JL, Serafini T, et al. (1996) Genetic analysis of Netrin genes in Drosophila: Netrins guide CNS commissural axons and peripheral motor axons. Neuron17: 203–215.
    [4] Rajasekharan S, Kennedy TE (2009) The netrin protein family. Genome Biol 10: 239. doi: 10.1186/gb-2009-10-9-239
    [5] Poon VY, Klassen MP, Shen K (2008) UNC-6/netrin and its receptor UNC-5 locally exclude presynaptic components from dendrites. Nature 455: 669. doi: 10.1038/nature07291
    [6] Stein E, Zou Y, Poo M-m, et al. (2001) Binding of DCC by netrin-1 to mediate axon guidance independent of adenosine A2B receptor activation. Science 291: 1976–1982. doi: 10.1126/science.1059391
    [7] Bashaw GJ, Goodman CS (1999) Chimeric axon guidance receptors: The cytoplasmic domains of slit and netrin receptors specify attraction versus repulsion. Cell 97: 917–926. doi: 10.1016/S0092-8674(00)80803-X
    [8] Liu Y, Bhowmick T, Liu Y, et al. (2018) Structural basis for Draxin-Modulated Axon guidance and fasciculation by Netrin-1 through DCC. Neuron 97: 1261–1267. doi: 10.1016/j.neuron.2018.02.010
    [9] Xing Y, Lai J, Liu X, et al. (2017) Netrin-1 restores cell injury and impaired-angiogenesis in vascular endothelial cells. J Mol Endocrinol: JME-16-0239.
    [10] Howard LJ, Brown HE, Wadsworth BC, et al. (2017) Midline axon guidance in the Drosophila embryonic central nervous system, In: Seminars in Cell & Developmental Biology, Elsevier.
    [11] Hand RA, Kolodkin AL (2017) Netrin-mediated axon guidance to the CNS midline revisited. Neuron 94: 691–693. doi: 10.1016/j.neuron.2017.05.012
    [12] Llambi F, Causeret F, Bloch‐Gallego E, et al. (2001) Netrin‐1 acts as a survival factor via its receptors UNC5H and DCC. EMBO J 20: 2715–2722.
    [13] Mehlen P, Delloye-Bourgeois C, Chédotal A (2011) Novel roles for Slits and netrins: Axon guidance cues as anticancer targets? Nature Rev Cancer 11: 188. doi: 10.1038/nrc3005
    [14] Wang W, Reeves WB, Ramesh G (2009) Netrin-1 increases proliferation and migration of renal proximal tubular epithelial cells via the UNC5B receptor. Am J Physiol Renal Physiol 296: F723–F9. doi: 10.1152/ajprenal.90686.2008
    [15] Ylivinkka I, Keski-Oja J, Hyytiäinen M (2016) Netrin-1: A regulator of cancer cell motility? Eur J Cell Biol 95: 513–520. doi: 10.1016/j.ejcb.2016.10.002
    [16] Ke X, Li Q, Xu L, et al. (2015) Netrin-1 overexpression in bone marrow mesenchymal stem cells promotes functional recovery in a rat model of peripheral nerve injury. J Biomed Res 29: 380.
    [17] Eming SA, Brachvogel B, Odorisio T, et al. (2007) Regulation of angiogenesis: Wound healing as a model. Progress Histochem Cytochem 42: 115–170. doi: 10.1016/j.proghi.2007.06.001
    [18] Park KW, Crouse D, Lee M, et al. (2004) The axonal attractant Netrin-1 is an angiogenic factor. Proc Nat Acad Sc i101: 16210–16215.
    [19] Sherman RA, Cooper EL (2018) Biotherapy: Medicinal maggots and invertebrate immunology from the Clinician's perspective. Adv Comp Immunol, Springer: 991–995.
    [20] Ramkhelawon B, Hennessy EJ, Ménager M, et al. (2014) Netrin-1 promotes adipose tissue macrophage retention and insulin resistance in obesity. Nature Med 20: 377. doi: 10.1038/nm.3467
    [21] Hadi T, Boytard L, Silvestro M, et al. (2018) Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cells. Nature Commun 9: 5022. doi: 10.1038/s41467-018-07495-1
    [22] Fournier AE, Strittmatter SM (2001) Repulsive factors and axon regeneration in the CNS. Curr Opin Neurobiol 11: 89–94. doi: 10.1016/S0959-4388(00)00178-1
    [23] Mumcuoglu KY, Miller J, Mumcuoglu M, et al. (2001) Destruction of bacteria in the digestive tract of the maggot of Lucilia sericata (Diptera: Calliphoridae). J Med Entomol 38: 161–166.
    [24] Alipour H, Raz A, Zakeri S, et al. (2017) Molecular characterization of matrix metalloproteinase-1 (MMP-1) in Lucilia sericata larvae for potential therapeutic applications. Electron J Biotechnol 29: 47–56. doi: 10.1016/j.ejbt.2017.06.007
    [25] Alipour H, Raz A, Zakeri S (2016) Therapeutic applications of collagenase (metalloproteases): A review. Asian Pac J Trop Biomed 6: 975–981. doi: 10.1016/j.apjtb.2016.07.017
    [26] Alipour H, Raz A, Djadid ND, et al. (2017) Lucilia sericata collagenase. Google Pat.
    [27] Akbarzadeh K, Rafinejad J, Alipour H, et al. (2012) Human myiasis in Fars province, Iran. Southeast Asian J Trop Med Public Health 43: 1205.
    [28] Rafinejad J, Akbarzadeh K, Rassi Y, et al. (2014) Traumatic myiasis agents in Iran with introducing of new dominant species, Wohlfahrtia magnifica (Diptera: Sarcophagidae). Asian Pacific J Trop Biomed 4: 451–455. doi: 10.12980/APJTB.4.2014C1029
    [29] Evans TA, Bashaw GJ (2012) Slit/Robo-mediated axon guidance in Tribolium and Drosophila: Divergent genetic programs build insect nervous systems. Dev Biol 1: 266–278
    [30] Čeřovský V, Žďárek J, Fučík V, et al. (2010) Lucifensin, the long-sought antimicrobial factor of medicinal maggots of the blowfly Lucilia sericata. Cell Mol Life Sci 67: 455–466. doi: 10.1007/s00018-009-0194-0
    [31] Telford G, Brown A, Seabra R, et al. (2010) Degradation of eschar from venous leg ulcers using a recombinant chymotrypsin from Lucilia sericata. Br J Dermatol 163: 523–531. doi: 10.1111/j.1365-2133.2010.09854.x
    [32] Feinstein J, Ramkhelawon B (2017) Netrins & Semaphorins: Novel regulators of the immune response. BBA-Mol Basis Dis 1863: 3183–3189. doi: 10.1016/j.bbadis.2017.09.010
    [33] Popel AS, Karagiannis ED (2015) Compositions having antiangiogenic activity and uses thereof. Google Pat .
    [34] Mediero A, Wilder T, Ramkhelawon B, et al. (2016) Netrin-1 and its receptor Unc5b are novel targets for the treatment of inflammatory arthritis. FASEB J 30: 3835–3844. doi: 10.1096/fj.201600615R
    [35] Maruyama K, Takemura N, Martino MM, et al. (2017) Netrins as prophylactic targets in skeletal diseases: A double-edged sword? Pharmacol Res 122: 46–52. doi: 10.1016/j.phrs.2017.05.011
    [36] Huttenlocher A, Poznansky MC (2008) Reverse leukocyte migration can be attractive or repulsive. Trends Cell Biol 18: 298–306.
    [37] Vigl B, Aebischer D, Nitschké M, et al. (2011) Tissue inflammation modulates gene expression of lymphatic endothelial cells and dendritic cell migration in a stimulus-dependent manner. Blood: blood-2010-12-326447.
    [38] Rőszer T (2015) Understanding the mysterious M2 macrophage through activation markers and effector mechanisms. Med Inflammation.
  • This article has been cited by:

    1. Laouedj Nadjia, Elaziouti Abdelkader, Taibi Mohamed, Investigation of microstructural, optical, and photocatalytic properties of sol–gel synthesized pristine SnO2 nanoscale particles, 2025, 131, 0947-8396, 10.1007/s00339-024-08221-z
  • Reader Comments
  • © 2019 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Metrics

Article views(5086) PDF downloads(698) Cited by(4)

Figures and Tables

Figures(6)

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return

Catalog