AIMS Genetics, 2019, 6(2): 17-35. doi: 10.3934/genet.2019.2.17.

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Physiological responses and transcriptome analysis of the Kochia prostrata (L.) Schrad. to seedling drought stress

1 Inner Mongolia Agricultural University, College of Grassland, Resources and Environment, Grass resource genetic breeding, China
2 ChiFeng University, Agricultural Science Research Institute; Grass resource genetic breeding, China
3 Inner Mongolia Academy of Agricultural and Animal Husbandry Sciences, Inner Mongolia Grass Research Center, Chinese Academy of Sciences, Grass resource genetic breeding, China
4 Inner Mongolia University for Nationalities, College of Animal Science and Technology, China

Kochia prostrata is a good forage plant, which has important economic and ecological value in arid and semi-arid regions of China. Drought is one of the main factors affecting its productivity. At present, there are few studies on the mechanism of drought resistance. In order to reveal the changes of physiological and biochemical indexes, stomatal structure and gene expression profiles of Kochia prostrata under drought treatment, the classical determination method and high-throughput Illumina Hiseq sequencing platform were applied to the control group (CK) and drought treatment group of Kochia prostrata. The results showed that under the condition of moderate to mild drought stress, the SOD activity reached the maximum value of 350.68 U/g min on the 5th day of stress, and under the condition of severe drought stress, the SOD activity reached the maximum on the 2nd day of stress. The accumulation of Proline remained at a high level on the 5th day of stress, and there was at least one epidermal cell interval between the two adult stomatal of the leaf epidermis, so that the evaporation shell of each stomatal did not overlap, it ensures the efficient gas exchange of the stomatal, indicating that the Kochia prostrata has strong drought resistance. A total of 1,177.46 M reads were obtained by sequencing, with a total of 352.25 Gbp data and Q30 of 85%. In the differential gene annotation to the biological process (BP), a total of 261 GO terms were enriched in the up-regulated genes, and a total of 231 GO terms were enriched in the down-regulated genes. The differentially expressed genes (DEGs) were obtained in 27 KEGG metabolic pathways, which laid a foundation for revealing the molecular mechanism of drought tolerance.
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Keywords Kochia prostrate; drought stress; stomatal; transcription factors; physiological changes

Citation: Xiaojuan Wang, Jianghong Wu, Zhongren Yang, Fenglan Zhang, Hailian Sun, Xiao Qiu, Fengyan Yi, Ding Yang, Fengling Shi. Physiological responses and transcriptome analysis of the Kochia prostrata (L.) Schrad. to seedling drought stress. AIMS Genetics, 2019, 6(2): 17-35. doi: 10.3934/genet.2019.2.17

References

  • 1. Engelbrecht BMJ, Comita LS, Condit R, et al. (2007) Drought sensitivity shapes species distribution patterns in tropical forests. Nature 447: 80–82.    
  • 2. Flato G, Gregory M, Jochem A, et al. (2013) Evaluation of climate models. In: Climate Change. The Physical Science Basis. Contribution of Working Group I to the Fifth Assessment Report of the Intergovernmental Panel on Climate Change, 5 Eds., Cambridge University Press, Seiten, 741–866.
  • 3. Anderegg WRL, Kane JM, Anderegg LDL (2012) Consequences of widespread tree mortality triggered by drought and temperature stress. Nat Clim Change 3: 30–36.
  • 4. Famiglietti JS, Rodell M (2013) Environmental science. Water in the balance. Science 340: 1300–1301.
  • 5. Pinheiro C, Chaves MM (2011) Photosynthesis and drought: Can we make metabolic connections from available data. J Exp Bot 62: 869–882.    
  • 6. Davis AM (1979) Forage quality of Prostrata Kochia compared with three browse species. Agron J 71: 822–824.    
  • 7. Wang XJ, Sun HL, Yang D, et al. (2015) Excellent feeding plants in arid region-Kochia prostrate. Grassl Prataculture 27: 10–12.
  • 8. Alata Z, Zhao SY, Li JZ (2010) The Kochia prostrate and its cultivation techniques. Anim Husb Feed Sci 31: 268–270.
  • 9. Yi F, Wang XJ, Qiu X, et al. (2016) Effect of Temperature, NaCI and PEG Stress on Germination and Seedling Growth of Kochia prostrate (L.) Schrad. var.horxina D.S. Wen Seed 35: 46–50.
  • 10. Hajihashemi S, Geuns JM, Ehsanpour AA (2013) Gene transcription of steviol glycoside biosynthesis in Stevia. rebaudiana Bertoni under polyethylene glycol, paclobutrazol and gibberellic acid treatments in vitro. Acta Physiol Plant 35: 2009–2011.
  • 11. He Q, Zhao S, Ma Q, et al. (2014) Endogenous salicylic acid levels and signaling positively regulate Arabidopsis response to polyethylene glycol-simulated drought stress. Plant Growth Regul 33: 871–880.    
  • 12. Abbaspour J, Ehsanpour AA (2016) Physiological targets of salicylic acid on Artemisia aucheri BOISS as a medicinaland aromatic plant grown under in vitro drought stress. Bot Stud 57: 39.    
  • 13. Wang X, Huang J (2015) Plant Physiology and Biochemistry Experiment Technology, In: Wang X, Huang J, Principles and Techniques of Plant Physiology and Biochemistry. 3 Eds., Beijing: Higher Education Press, 131–133.
  • 14. Li HS (2000) Principles and Techniques of Plant Physiology and Biochemistry Experiment. 2 Eds., Beijing: Higher Education Press, 46–57.
  • 15. Young MD, Wakefield MJ, Smyth GK, et al. (2010) Gene ontology analysis for RNA-seq: Accounting for selection bias. Genome Biol 11: R14.    
  • 16. Kanehisa M, Araki M, Goto S, et al. (2008) KEGG for linking genomes to life and the environment. Nucleic Acids Res 36: D480–D484.
  • 17. Mao X, Cai T, Olyarchuk JG, et al. (2005) Automated genome annotation and pathway identification using the KEGG Orthology (KO) as a controlled vocabulary. Bioinformatics 21: 3787–3793.    
  • 18. Chandrasekar V, Sairam RK, Srivastava GC (2000) Physiological and biochemical responses of hexaploid and hetraploid wheat to drought tress. Agron Crop Sci 185: 219–227.
  • 19. McMichael BL, Elmore CD (1997) Proline accumulation in water stressed cotton leaves. Crop Sci 17: 905–908.
  • 20. Liu FL, Jensen CR, Anderson MN, et al. (2004) Drought stress effect on carbohydrate concentration in soybean leaves andpods during early reproductive development: Its implication in altering pod set. Field Crops Res 86: 1–13.    
  • 21. Ohashi Y, Nakayama N, Saneoka H, et al. (2006) Effects of drought stress on photosynthetic gas exchange, chlorophyll fluorescence and stem diameter of soybean plants. Biologia Plantarum 50: 138–141.    
  • 22. Taylor SH, Franks PJ, Hulme SP, et al. (2012) Photosynthetic pathway and ecological adaptation explain stomatal trait diversity amongst grasses. New Phytologist 193: 387–396.    
  • 23. Franks PJ, Beerling DJ (2009) Maximum leaf conductance driven by CO2 effects on stomatal size and density over geologic time. Proceedings of the National Academy of Sciences of the United States of America 106: 10343–10347.    
  • 24. Liu W, Ji SX, Fang XL, et al. (2013) Protein kinase LTRPK1 influences cold adaptation and microtubule stability in rice. Plant Growth Regul 32: 483–490.    
  • 25. Park SY, Fung P, Nishimura N, et al. (2009) Abscisic acid inhibits type 2C protein phosphatases via the PYR/PYL family of START proteins. Science 324: 1068–1071.
  • 26. Zhang X, Miao YC, An GY, et al. (2001) Kannels inhibited by hydrogen peroxide mediate abscisic acid signaling in guard cells. Cell Res 11: 195–202    
  • 27. Rodriguez MC, Petersen M, Mundy J (2010) Mitogen-activated protein kinase signaling in plants. Annu. Rev Plant Biol 61: 621–649.    
  • 28. Bowler C, Slooten L, Vandenbranden S, et al. (1991) Manganese superoxide dismutase can reduce cellular damage mediated by oxygen radicals in transgenic plants. EMBO J 10: 1723–1732.    
  • 29. Jin H, Huang F, Cheng H, et al. (2013) Overexpression of the GmNAC2 gene, an NAC transcription factor, reduces abiotic stress tolerance in tobacco. Plant Mol Biol Rep 31: 435–442.    
  • 30. Sun J, Hu W, Zhou R, et al. (2015) The Brachypodium distachyon BdWRKY36 gene confers tolerance to drought stress in transgenic tobacco plants. Plant Cell Rep 34: 23–35.    
  • 31. Shafi A, Chauhan R, Gill T, et al. (2015) Expression of SOD and APX genes positively regulates secondary cell wall biosynthesis and promotes plant growth and yield in Arabidopsis under salt stress. Plant Mol Biol 87: 615–631.    
  • 32. Xu K, Chen S, Li T, et al. (2015) OsGRAS23, a rice GRAS transcription factor gene, is involved in drought stress response through regulating expression of stress-responsive genes. BMC Plant Biol 15: 141.    
  • 33. Jin H, Huang F, Cheng H, et al. (2015) Overex-pression of the GmNAC2 gene, an NAC transcription factor, reduces abiotic stress tolerance in tobacco. Plant Mol Biol Rep 31: 435–442.

 

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