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Ethanol production from hot-water sugar maple wood extract hydrolyzate: fermentation media optimization for Escherichia coli FBWHR

Department of Paper and Bioprocess Engineering, State University of New York—College of Environmental Science and Forestry, Syracuse, NY 13210, USA

Special Issues: Integrated biochemical conversion of biomass into chemicals and fuels

We report the first time statistical study of the optimization for ethanol production from hot-water sugar maple hemicellulosic wood hydrolyzate by Escherichia coli FBWHR. Response surface methodology was employed to investigate the effect of fermentation media on the ethanol production from concentrated hot-water sugar maple hemicellulosic wood extract hydrolyzate by Escherichia coli FBWHR. The critical media components were firstly selected according to Plackett–Burman design and further optimized by central composite design. Based on the response surface analysis, the optimum concentrations of the significant components were obtained: yeast extract, 10.19 g/L; tryptone, 14.55 g/L; Na2HPO4·7H2O, 23.21 g/L; KH2PO4, 5 g/L and NH4Cl, 2 g/L. An ethanol concentration of 15.23 ± 0.21 g/L was achieved under the optimized media, which agreed with the predicted value. Ethanol production was enhanced to 22.18 ± 0.13 g/L by scaling up the fermentation from shaker flask to 1.3 L bioreactor.
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1. Amidon T, Wood C, Shupe A, et al. (2008) Biorefinery: Conversion of woody biomass to chemicals, energy and materials. J Biobased Mater Bioenergy 2: 100-120.    

2. Liu S (2012) Utilization of Woody Biomass: Sustainability. J Bioprocess Eng Biorefinery 1: 129-139.    

3. Wang Y, Liu S (2012) Butadiene production from ethanol. J Bioprocess Eng Biorefinery 1: 33-43.    

4. Lokhorst A, Wildenborg I (2005) Introduction to CO2 geological storage: Classification of storage options. Oil Gas Sci Technol 60: 513-515.    

5. Balat M, Balat H (2009) Recent trends in global production and utilization of bio-ethanol fuel. Appl Energ 86: 2273-2282.    

6. Qureshi N, Dien B, Liu S, et al. (2012) Genetically engineered Escherichia coli FBR5: part I. Comparison of high cell density bioreactors for enhanced ethanol production from xylose. Biotechnol Prog 2: 1167-1178.

7. Srirangan K, Akawi L, Moo-Young M, et al. (2012) Towards sustainable production of clean energy carriers from biomass resources. Appl Energ 100: 172-186.    

8. Schenck A, Berglin N, Uusitalo J (2013) Ethanol from Nordic wood raw material by simplified alkaline soda cooking pre-treatment. Appl Energ 102: 229-240.    

9. Wang Y, Liu S (2012) Pretreatment technologies for biological and chemical conversion of woody biomass. TAPPI J 11: 9-16.

10. Gírio F, Fonseca C, Carvalheiro F, et al. (2010) Hemicelluloses for fuel ethanol: A review. Bioresour Technol 101: 4775-4800.    

11. Wang Y, Liu Z, Chatsko M, et al. (2013) Ethanol fermentation by Escherichia coli FBWHR using hot-water sugar maple wood extract hydrolyzate as substrate: A batch fermentation and kinetic study. J Bioprocess Eng Biorefinery 2: 1-7.    

12. Tholudur A, Sorensen T, Zhu X, et al. (2005) Using Design of Experiments To Assess Escherichia coli Fermentation Robustness. BioProcess Int 3: 1-4.

13. Cockshott A, Sullivan G (2001) Improving the fermentation medium for Echinocandin B production. Part I: sequential statistical experimental design. Process Biochem 36: 647-660.

14. Chen P, Chiang C, Chao Y (2010) Medium optimization and production of secreted Renilla luciferase in Bacillus subtilis by fed-batch fermentation. Biochem Eng J 49: 395-400.    

15. Plackett R, Burman J (1946) The design of optimum multifactorial experiments. Biometrika 33: 305-325.    

16. Guo Y, Xu J, Zhang Y, et al. (2010) Medium optimization for ethanol production with Clostridium autoethanogenum with carbon monoxide as sole carbon source. Bioresour Technol 101: 8784-9.    

17. Chauhan K, Trivedi U, Patel K (2007) Statistical screening of medium components by Plackett-Burman design for lactic acid production by Lactobacillus sp. KCP01 using date juice. Bioresour Technol 98: 98-103.

18. Mei X, Liu R, Shen F, et al. (2009) Optimization of fermentation conditions for the production of ethanol from stalk juice of sweet sorghum by immobilized yeast using response surface methodology. Energ Fuels 23: 487-91.    

19. Pereira F, Guimarães P, Teixeira J, et al. (2010) Optimization of low-cost medium for very high gravity ethanol fermentations by Saccharomyces cerevisiae using statistical experimental designs. Bioresour Technol 101: 7856-63.    

20. Grahovac J, Dodic J, Jokic A, et al. (2012) Optimization of ethanol production from thick juice: A response surface methodology approach. Fuel 93: 221-228.    

21. Wang R, Ji Y, Melikoglu M, et al. (2007) Optimization of innovative ethanol production from wheat by response surface methodology. Trans IChemE 85: 404-412.    

22. Yu J, Zhang X, Tan T (2009) Optimization of media conditions for the production of ethanol from sweet sorghum juice by immobilized Saccharomyces cerevisiae. Biomass Bioenerg 33: 521-526.    

23. Dagnino E, Chamorro E, Romano S, et al. (2013) Optimization of the acid pretreatment of rice hulls to obtain fermentable sugars for bioethanol production. Ind Crop Prod 42: 363-368.    

24. Cao W, Liu R (2013) Screening and optimization of trace elements supplement in sweet sorghum juice for ethanol production. Biomass Bioenerg 50: 45-51.    

25. Liang L, Zheng Y, Shen Y (2008) Optimization of β-alanine production from β-aminopropionitrile by resting cells of Rhodococcus sp. G20 in a bubble column reactor using response surface methodology. Process Biochem 43: 758-764.

26. Thomas K, Ingledew W (1990) Fuel alcohol production: effects of free amino nitrogen on fermentation of very-high-gravity wheat mashes. Appl Environ Microbiol 56: 2046-2050.

27. Bakonyi P, Nemestóthy N, Lövitusz É, et al. (2011) Application of Plackett-Burman experimental design to optimize biohydrogen fermentation by E. coli (XL1-BLUE). Int J Hydrogen Energ 36: 13949-13954.    

28. Suárez D, Liria C, Kilikian B (1998) Effect of yeast extract on Escherichia coli growth and acetic acid production. World J Microbiol Biotechnol 14: 331-335.    

29. He J, Zhen Q, Qiu N, et al. (2009) Medium optimization for the production of a novel bioflocculant from Halomonas sp. V3áusing response surface methodology. Bioresour Technol 100: 5922-5927.

30. Haider M, Pakshirajan K (2007) Screening and optimization of media constituents for enhancing lipolytic activity by a soil microorganism using statistically designed experiments. Appl Biochem Biotechnol 141: 377-390.    

Copyright Info: © 2015, Shijie Liu, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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