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Double casting prototyping with a thermal aging step for fabrication of 3D microstructures in poly(dimethylsiloxane)

  • Received: 23 September 2016 Accepted: 16 November 2016 Published: 22 November 2016
  • The paper describes a cheap and accessible technique of a poly(dimethylsiloxane) (PDMS) master treatment by thermal aging as a step of double casting microfabrication process. Three-dimensional PDMS microstructures could have been achieved using this technique. It was proved, that thermal aging changes nanotopology of a PDMS surface and thus enhances efficiency of double casting prototyping. The thermally aged PDMS master could have been used for multiple and correct replication of over 98% of the fabricated microstructures. Moreover, lack of chemical modification preserved the biocompatibility of PDMS devices. The fabricated microstructures were successfully utilized for 3D cell culture.

    Citation: Karina Kwapiszewska, Kamil Żukowski, Radosław Kwapiszewski, Zbigniew Brzózka. Double casting prototyping with a thermal aging step for fabrication of 3D microstructures in poly(dimethylsiloxane)[J]. AIMS Biophysics, 2016, 3(4): 553-562. doi: 10.3934/biophy.2016.4.553

    Related Papers:

  • The paper describes a cheap and accessible technique of a poly(dimethylsiloxane) (PDMS) master treatment by thermal aging as a step of double casting microfabrication process. Three-dimensional PDMS microstructures could have been achieved using this technique. It was proved, that thermal aging changes nanotopology of a PDMS surface and thus enhances efficiency of double casting prototyping. The thermally aged PDMS master could have been used for multiple and correct replication of over 98% of the fabricated microstructures. Moreover, lack of chemical modification preserved the biocompatibility of PDMS devices. The fabricated microstructures were successfully utilized for 3D cell culture.


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    [1] Chudy M, Grabowska I, Ciosek P, et al. (2009) Miniaturized tools and devices for bioanalytical applications: an overview. Anal Bioanal Chem 395: 647–668. doi: 10.1007/s00216-009-2979-2
    [2] Kwapiszewski R, Skolimowski M, Ziółkowska K, et al. (2011) A microfluidic device with fluorimetric detection for intracellular components analysis. Biomed Microdevices 13: 431–440. doi: 10.1007/s10544-011-9511-0
    [3] Ni M, Tong W, Choudhury D, et al. (2009) Cell culture on MEMS platforms: a review. Int J Mol Sci 10: 5411–5441. doi: 10.3390/ijms10125411
    [4] McDonald J, Whitesides G (2002) Poly(dimethylsiloxane) as a material for fabricating microfluidic devices. Acc Chem Res 35: 491–499. doi: 10.1021/ar010110q
    [5] Regehr K, Domenech M, Koepsel J, et al. (2009) Biological implications of polydimethylsiloxane-based microfluidic cell culture. Lab Chip 9: 2132–2139. doi: 10.1039/b903043c
    [6] Quake S, Scherer A (2000) From micro-to nanofabrication with soft materials. Science 290: 1536–1540.
    [7] Velve-Casquillas G, Le Berre M, Piel M, et al.(2010) Microfluidic tools for cell biological research. Nano Today 5: 28–47
    [8] Leclerc E, Sakai Y, Fujii T (2003) Cell Culture in 3-Dimensional Microfluidic Structure of PDMS (polydimethylsiloxane). Biomed Microdevices 5: 109–114. doi: 10.1023/A:1024583026925
    [9] Ziółkowska K, Jędrych E, Kwapiszewski R, et al. (2010) PDMS/glass microfluidic cell culture system for cytotoxicity tests and cells passage. Sensor Actuat B 145: 533–542 doi: 10.1016/j.snb.2009.11.010
    [10] Ziółkowska K, Kwapiszewski R, Brzózka Z (2011) Microfluidic devices as tools for mimicking the in vivo environment. New J Chem 35: 979–990.
    [11] Ziółkowska K, Kwapiszewski R, Stelmachowska A, et al. (2012) Development of a three-dimensional microfluidic system for long-term tumor spheroid culture. Sensor Actuat B 173: 908–913. doi: 10.1016/j.snb.2012.07.045
    [12] Ziółkowska K, Stelmachowska A, Kwapiszewski R, et al. (2013) Long-term three-dimensional cell culture and anticancer drug activity evaluation in a microfluidic chip. Biosensor Bioelectron 40: 68–74. doi: 10.1016/j.bios.2012.06.017
    [13] Kwapiszewska K, Michalczuk A, Rybka M, et al. (2014) A microfluidic-based platform for tumour spheroid culture, monitoring and drug screening. Lab Chip 14: 2096–2104. doi: 10.1039/C4LC00291A
    [14] McDonald J, Duffy D, Anderson J, et al. (2000) Fabrication of microfluidic systems in poly(dimethylsiloxane). Electrophoresis 21: 27–40.
    [15] Kim J, Heo J, Crooks R (2006) Hybridization of DNA to bead-immobilized probes confined within a microfluidic channel. Langmuir 22: 10130–10134. doi: 10.1021/la0616956
    [16] Adams M, Johnston M, Scherer A, et al. (2005) Polydimethylsiloxane based microfluidic diode. J Micromech Microeng 15: 1517–1521. doi: 10.1088/0960-1317/15/8/020
    [17] Lim C, Low H, Ng J, et al. (2009) Fabrication of three-dimensional hemispherical structures using photolithography. Microfluid Nanofluid 7: 721–726.
    [18] Huikko K, Ostman P, Grigoras K, et al. (2003) Poly(dimethylsiloxane) electrospray devices fabricated with diamond-like carbon–poly(dimethylsiloxane) coated SU-8 masters. Lab Chip 3: 67–72. doi: 10.1039/B300345K
    [19] Gitlin L, Schulze P, Belder D (2009) Rapid replication of master structures by double casting with PDMS. Lab Chip 9: 3000–3002.
    [20] Zhuang G, Kutter J (2011) Anti-stiction coating of PDMS moulds for rapid microchannel fabrication by double replica moulding. J Micromech Microeng 21: 105020. doi: 10.1088/0960-1317/21/10/105020
    [21] Eddington D, Puccinelli J, Beebe D (2006) Thermal aging and reduced hydrophobic recovery of polydimethylsiloxane. Sensor Actuat B 114: 170–172.
    [22] Briones M, Honda T, Yamaguchi Y, et al. (2006) A Practical Method for Rapid Microchannel Fabrication in Polydimethylsiloxane by Replica Molding without Using Silicon Photoresist. J Chem Eng Jpn 39: 1108–1114. doi: 10.1252/jcej.39.1108
    [23] Koerner T, Brown L, Xie R, et al. (2005) Epoxy resins as stamps for hot embossing of microstructures and microfluidic channels. Sensor Actuat B 107: 632–639. doi: 10.1016/j.snb.2004.11.035
    [24] Wong I, Ho C (2009) Surface molecular property modifications for poly(dimethylsiloxane) (PDMS) based microfluidic devices. Microfluid Nanofluid 7: 291–306. doi: 10.1007/s10404-009-0443-4
    [25] Zhou J, Ellis A, Voelcker N (2010) Recent developments in PDMS surface modification for microfluidic devices. Electrophoresis 31: 2–16. doi: 10.1002/elps.200900475
    [26] Leite C, Soares R, Goncalves M, et al. (1994) Surface dynamics of polydimethylsiloxane rubber. Polymer 35: 3173–3177. doi: 10.1016/0032-3861(94)90118-X
    [27] Jeong O, Konishi S (2011) Controlling the size of replicable polydimethylsiloxane (PDMS) molds/stamps using a stepwise thermal shrinkage process. Microelectron Eng 88: 2286–2289. doi: 10.1016/j.mee.2010.12.005
    [28] Jokinen V, Suvato P, Franssila S (2012) Oxygen and nitrogen plasma hydrophilization and hydrophobic recovery of polymers. Biomicrofluidics 6: 016501–016510.
    [29] Liu M, Chen Q (2007) Characterization study of bonded and unbonded polydimethylsiloxane aimed for bio-microelectromechanical systems-related applications. J Micro/Nanolith MEMS MOEMS 6: 023008. doi: 10.1117/1.2731381
    [30] Liu M, Sun J, Chen Q (2009) Influences of heating temperature on mechanical properties of polydimethylsiloxane. Sensor Actuat A 151: 42–45. doi: 10.1016/j.sna.2009.02.016
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