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Poly-ε-caprolactone electrospun nanofiber mesh as a gene delivery tool

1 Department of Orthopaedic Surgery, University of Kansas School of Medicine, Wichita, KS, USA
2 Department of Biological Sciences, Wichita State University, Wichita, KS, USA
3 Department of Mechanical Engineering, Wichita State University, Wichita, KS, USA
4 Department of Orthopaedic Surgery, Binzhou Medical College Affiliated Hospital, Binzhou, Shandong, China
5 Department of Mechatronics Engineering, İstanbul Commerce University, Istanbul, Turkey
6 Department of Medicine, the First Affiliated Hospital of Shihezi University, Xinjiang, China

# These authors contributed equally on this work.

Special Issues: Utilization of DNA in nanotechnology

Poly-ε-caprolactone (PCL) is a biodegradable aliphatic polyester which plays critical roles in tissue engineering, such as scaffolds, drug and protein delivery vehicles. PCL nanofiber meshes fabricated by electrospinning technology have been widely used in recent decade. The objective of this study intends to develop a gene-tethering PCL-nanofiber mesh that can be used as a wrapping material during surgical removal of primary bone tumors, and as a gene delivery tool to provide therapeutic means for tumor recurrence. Non-viral plasmid vector encoding green fluorescent protein (eGFP) was incorporated into PCL nanofibers by electron-spinning technique to form multilayer nano-meshes. Our data demonstrated that PCL nanofiber mesh possessed benign biocompatibility in vitro. More importantly, pCMVb-GFP plasmid-linked electrospun nanofiber mesh successfully released the GFP marker gene and incorporated into the co-cultured fibroblast cells, and consequently expressed the transgene product at transcriptional and translational levels. Further investigation is warranted to characterize the therapeutic influence and long-term safety issue of the PCL nanofiber mesh as a gene delivery tool and therapeutic device in orthopedic oncology.
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Keywords electrospun nanofiber; EGFP; PCL; tissue engineering; gene delivery

Citation: Jianhao Jiang, Muhammet Ceylan, Yi Zheng, Li Yao, Ramazan Asmatulu, Shang-You Yang. Poly-ε-caprolactone electrospun nanofiber mesh as a gene delivery tool. AIMS Bioengineering, 2016, 3(4): 528-537. doi: 10.3934/bioeng.2016.4.528


  • 1. Weng CJ, Yen GC (2012) Chemopreventive effects of dietary phytochemicals against cancer invasion and metastasis: phenolic acids, monophenol, polyphenol, and their derivatives. Cancer Treat Rev 38: 76–87.    
  • 2. Longhi A, Errani C, De Paolis M, et al. (2006) Primary bone osteosarcoma in the pediatric age: state of the art. Cancer Treat Rev 32: 423–436.
  • 3. Oshima Y, Sasaki Y, Negishi H, et al. (2007) Antitumor effect of adenovirus-mediated p53 family gene transfer on osteosarcoma cell lines. Cancer Biol Ther 6: 1058–1066.    
  • 4. Tsuji H, Kawaguchi S, Wada T, et al. (2003) Concurrent induction of T-cell activation and apoptosis of osteosarcoma cells by adenovirus-mediated B7-1/Fas chimeric gene transfer. Cancer Gene Ther 10: 717–725.
  • 5. Green JJ (2012) 2011 Rita Schaffer lecture: nanoparticles for intracellular nucleic acid delivery. Ann Biomed Eng 40: 1408–1418.    
  • 6. Cohen-Sacks H, Elazar V, Gao J, et al. (2004) Delivery and expression of pDNA embedded in collagen matrices. J Control Release 95: 309–320.    
  • 7. Luu YK, Kim K, Hsiao BS, et al. (2003) Development of a nanostructured DNA delivery scaffold via electrospinning of PLGA and PLA-PEG block copolymers. J Control Release 89: 341–353.    
  • 8. Kai D, Prabhakaran MP, Stahl B, et al. (2012) Mechanical properties and in vitro behavior of nanofiber-hydrogel composites for tissue engineering applications. Nanotechnology 23: 095705.    
  • 9. Yohe ST, Herrera VL, Colson YL, et al. (2012) 3D superhydrophobic electrospun meshes as reinforcement materials for sustained local drug delivery against colorectal cancer cells. J Control Release 162: 92–101.    
  • 10. Capkin M, Cakmak S, Kurt FO, et al. (2012) Random/aligned electrospun PCL/PCL-collagen nanofibrous membranes: comparison of neural differentiation of rat AdMSCs and BMSCs. Biomed Mater 7: 045013.    
  • 11. Vadala G, Mozetic P, Rainer A, et al. (2012) Bioactive electrospun scaffold for annulus fibrosus repair and regeneration. Eur Spine J 21: 20–26.    
  • 12. Yu H, VandeVord PJ, Mao L, et al. (2009) Improved tissue-engineered bone regeneration by endothelial cell mediated vascularization. Biomaterials 30: 508–517.    
  • 13. Croisier F, Duwez AS, Jerome C, et al. (2012) Mechanical testing of electrospun PCL fibers. Acta Biomater 8: 218–224.    
  • 14. Kamimura K, Suda T, Zhang G, et al. (2011) Advances in gene delivery systems. Pharmaceut Med 25: 293–306.
  • 15. Andreou LV (2013) Isolation of plasmid DNA from bacteria. Methods Enzymol 529: 135–142.    
  • 16. Hanahan D, Weinberg RA, (2011) Hallmarks of cancer: the next generation. Cell 144: 646–674.
  • 17. Sun DX, Liao GJ, Liu KG, et al. (2015) Endosialinexpressing bone sarcoma stemlike cells are highly tumorinitiating and invasive. Mol Med Rep 12: 5665–5670.
  • 18. Bougeret C, Virone-Oddos A, Adeline E, et al. (2000) Cancer gene therapy mediated by CTS1, a p53 derivative: advantage over wild-type p53 in growth inhibition of human tumors overexpressing MDM2. Cancer Gene Ther 7: 789–798.    
  • 19. Sim GC, Radvanyi L (2014) The IL-2 cytokine family in cancer immunotherapy. Cytokine Growth F R 25: 377–390.    
  • 20. Subramanian A, Krishnan UM, Sethuraman S (2012) Fabrication, characterization and in vitro evaluation of aligned PLGA-PCL nanofibers for neural regeneration. Ann Biomed Eng 40: 2098–2110.    
  • 21. Nitya G, Nair GT, Mony U, et al. (2012) In vitro evaluation of electrospun PCL/nanoclay composite scaffold for bone tissue engineering. J Mater Sci Mater Med 23: 1749–1761.    
  • 22. Engin K, Leeper DB, Cater JR, et al. (1995) Extracellular pH distribution in human tumours. Int J Hyperthermia 11: 211–216.    
  • 23. Sharma S, Mohanty S, Gupta D, et al. (2011) Cellular response of limbal epithelial cells on electrospun poly-epsilon-caprolactone nanofibrous scaffolds for ocular surface bioengineering: a preliminary in vitro study. Mol Vis 17: 2898–2910.
  • 24. Li L, Li G, Jiang J, et al. (2012) Electrospun fibrous scaffold of hydroxyapatite/poly (epsilon-caprolactone) for bone regeneration. J Mater Sci Mater Med 23: 547–554.
  • 25. Son YJ and Yoo HS (2012) Dexamethasone-incorporated nanofibrous meshes for antiproliferation of smooth muscle cells: thermally induced drug-loading strategy. J Biomed Mater Res A 100: 2678–2685.
  • 26. Saraf A, Hacker M C, Sitharaman B, et al. (2008) Synthesis and conformational evaluation of a novel gene delivery vector for human mesenchymal stem cells. Biomacromolecules 9: 818–827.    
  • 27. Dubsky M, Kubinova S, Sirc J, et al. (2012) Nanofibers prepared by needleless electrospinning technology as scaffolds for wound healing. J Mater Sci Mater Med 23: 931–941.    
  • 28. Kim HJ, Choi EY, Oh JS, et al. (2000) Possibility of wound dressing using poly(L-leucine)/poly(ethylene glycol)/poly(L-leucine) triblock copolymer. Biomaterials 21: 131–141.    


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Copyright Info: 2016, Shang-You Yang, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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