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Assessment of heavy metal tolerance and hexavalent chromium reducing potential of Corynebacterium paurometabolum SKPD 1204 isolated from chromite mine seepage

Microbiology Laboratory, Department of Botany, University of Calcutta, Kolkata 700019, India

Topical Section: Bioremediation of contaminated soil

Corynebacterium paurometabolum SKPD 1204 (MTCC 8730), a heavy metal tolerant and chromate reducing bacterium isolated from chromite mine seepage of Odisha, India has been evaluated for chromate reduction under batch culture. The isolate was found to tolerate metals like Co(II), Cu(II), Ni(II), Mn(II), Zn(II), Fe(III) and Hg(II) along with Cr(VI) and was resistant to different antibiotics as evaluated by disc-diffusion method. The isolate, SKPD 1204 was found to reduce 62.5% of 2 mM Cr(VI) in Vogel Bonner broth within 8 days of incubation. Chromate reduction capability of SKPD 1204 decreased with increase in Cr(VI) concentration, but increased with increase in cell density and attained its maximum at 1010 cells/mL. Chromate reducing efficiency of SKPD 1204 was promoted in the presence of glycerol and glucose, while the highest reduction was recorded at pH 7.0 and 35 °C. The reduction process was inhibited by divalent cations Zn(II), Cd(II), Cu(II), and Ni(II), but not by Mn(II). Anions like nitrate, phosphate, sulphate and sulphite was found to be inhibitory to the process of Cr(VI) reduction. Similarly, sodium fluoride, carbonyl cyanide m-chlorophenylhydrazone, sodium azide and N, N,-Di cyclohexyl carboiimide were inhibitory to chromate reduction, while 2,4-dinitrophenol appeared to be neither promotive nor inhibitory to the process.
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Keywords Corynebacterium paurometabolum; mine seepage; metal tolerance; antibiotic resistance index; Cr(VI) bioremediation

Citation: Satarupa Dey, Amal Kanti Paul. Assessment of heavy metal tolerance and hexavalent chromium reducing potential of Corynebacterium paurometabolum SKPD 1204 isolated from chromite mine seepage. AIMS Bioengineering, 2016, 3(3): 337-351. doi: 10.3934/bioeng.2016.3.337


  • 1. Mondal SK (2009) Chromite and PGE Deposits of Mesoarchaean Ultramafic-Mafic Suites within the Greenstone Belts of the Singhbhum Craton,India: Implications for Mantle Heterogeneity and Tectonic Setting. J Geo SocIndia 73: 36–51.
  • 2. Godgul G, Sahu KC (1995) Chromium contamination from chromium mine. Environ Geol 25: 251–257.    
  • 3. Gibb HJ, Lee PS, Pinsky PF, et al. (2000a) Lung cancer among workers in chromium chemical production. Am J Indus Med 38: 115–126.
  • 4. Gibb HJ, Lee PS, Pinsky PF, et al. (2000b) Clinical findings of irritation among chromium chemical production workers. Am J Indus Med 38: 127–131.
  • 5. Fiol N, Escudero C, Villaescusa I (2008) Chromium sorption and Cr(VI) reduction to Cr(III) by Grape Stalks and Yohimbe Bark. Biores Technol 99: 5030–5036.    
  • 6. Cheung KH, Gu JD (2003) Reduction of chromate (CrO42-) by an enrichment consortium and an isolate of marine sulfate-reducing bacteria. Chemosphere 52: 1523–1529.    
  • 7. Dey S, Paul AK, (2012) Optimization of cultural conditions for growth associated chromate reduction by Arthrobacter sp. SUK 1201 isolated from chromite mine overburden. J Haz Mat 213–214: 200–206.
  • 8. Dhal B, Thatoi HN, Das NN et al. (2010) Reduction of hexavalent chromium by Bacillus sp. isolated from chromite mine soils and characterization of reduced product. J Chem Technol Biotechnol 85: 1471–1479.
  • 9. Samuel J, Paul ML, Pulimi M et al. (2012) Hexavalent chromium bioremoval through adaptation and consortia development from sukinda chromite mine isolates. Ind Eng Chem Res 51: 3740–3749.    
  • 10. Wang YT, Xiao C (1995) Factors affecting hexavalent chromium reduction in pure cultures of bacteria. Water Res 29: 2467–2474.    
  • 11. Park CH, Keyhan B, Wielinga B et al. (2000) Purification to homogeneity and characterization of a novel Pseudomonas putida chromate reductase, Appl Environ Microbiol 66: 1788–1795.
  • 12. Lowry OH, Rosebrough NJ, Farr AL et al. (1951) Protein measurements with the folin phenol reagent. J Biol Chem 193: 265–275.
  • 13. Calomiris JJ, Armstrong TL, Seidler RJ (1984) Association of metal-tolerance with multiple antibiotic resistance of bacteria isolated from drinking water, Appl Environ Microbiol 47: 1238–1242
  • 14. Camargo FAO, Okeke BC, Bento FM, et al. (2003) Chromate reduction by chromium-resistant bacteria isolated from soils contaminated with dichromate. J Environ Qual 32: 1228–1233.    
  • 15. Dey S, Paul AK, (2010) Occurrence and evaluation of chromium reducing bacteria in seepage water from chromite mine quarries of Orissa,India. J Water Res Protect 2: 380–388.    
  • 16. Dey S, Pandit B, Paul AK (2014). Reduction of hexavalent chromium by viable whole cells of chromium resistant bacteria isolated from chromite mining environment. J Min 2014: 941341.
  • 17. Steinhaus EA (1941) A study of the bacteria associated with thirty species of insects. J Bacteriol 42: 757–790.
  • 18. Viti P, Pace A, Giovannetti L (2003) Characterisation of chromium-resistant bacteria isolated from chromium-contaminated soil by tannery activity Curr Microbiol 46: 1–5.
  • 19. Tahri Joutey N, Bahafid W, Sayel H, et al. (2014) Hexavalent chromium removal by a novel Serratia proteamaculans isolated from the bank of Sebou River (Morocco). Environ Sci Pollut Res 21: 3060–3072    
  • 20. Kang C, Wu P, Li Y, et al. (2014) Estimates of heavy metal tolerance and chromium(VI) reducing ability of Pseudomonas aeruginosa CCTCC AB93066: chromium(VI) toxicity and environmental parameters optimization. World J Microbiol Biotechnol 30: 2733–2746    
  • 21. Rehman A, Zahoor A, Muneer B, et al. (2008) Chromium tolerance and reduction potential of a Bacillus sp.ev3 isolated from metal contaminated wastewater. Bull Environ Cont Toxicol 81: 25–29.
  • 22. Branco R, Chung AP, Johnston T, et al. (2008) The chromate-inducible chrBACF operon from the transposable element TnOtChr confers resistance to chromium(VI) and superoxide. J Bacteriol 190: 6996–7003.    
  • 23. Pal A, Paul AK (2004) Aerobic chromate reduction by chromium resistant bacteria isolated from serpentine soil. Microbiol Res 159: 347–354.    
  • 24. Jain PK, Ramachandran S, Shukla V, et al. (2009) Characterization of metal and antibiotic resistance in a bacterial population isolated from copper mining industry. Int J Integ Biol 6: 57–61.
  • 25. Poopal AC, Laxman RS (2009) Studies on biological reduction of chromate by Streptomyces griseus. J Haz Mat 169: 539–545.    
  • 26. Das S, Mishra J, Das SK, et al (2014) Investigation on mechanism of Cr(VI) reduction and removal by Bacillus amyloliquefaciens, a novel chromate tolerant bacterium isolated from chromite mine soil. Chemosphere 96: 112–121.    
  • 27. Javaid M, Sultan S (2013) Plant growth promotion traits and Cr (VI) reduction potentials of Cr (VI) resistant Streptomyces strains. J Basic Microbiol 53: 420–428.    
  • 28. Bhattacharya A, Gupta A (2013) Evaluation of Acinetobacter sp. B9 for Cr(VI) resistance and detoxification with potential application in bioremediation of heavy-metals-rich industrial wastewater. Environ Sci Pollut Res 20: 6628–6637.
  • 29. Basu A, Ghosh SK, Saha R (2011) Effect of Some Non Functional Surfactants and Electrolytes on the Hexavalent Chromium Reduction by Glycerol: A Mechanistic Study. Tenside Surfactants Detergents 48: 453–458.    
  • 30. Sultan S, Hasnain S (2006) Characterization of Ochrobactrum intermedium strain STCr-5 manifesting high level Cr(VI) resistance and reduction potential. Enzyme Microbial Technol 39: 883–888.    
  • 31. Maret W (2013) Inhibitory zinc sites in enzymes. Biometals 26: 197–204.    
  • 32. Opperman DJ, Heerden EV (2007) Aerobic Cr(VI) reduction by Thermus scotoductus strain SA-01. J Appl Microbiol 103: 1907–1913.    
  • 33. Wani R, Kodam KM, Gawai KR, et al. (2007) Chromate reduction by Burkholderia cepacia MCMB-821 isolated from the pristine habitat of alkaline crater lake. Appl Microbiol Biotechnol 75: 627–632.    
  • 34. Alam MZ, Ahmad S (2011) Toxic chromate reduction by resistant and sensitive bacteria isolated from tannery effluent contaminated soil. Ann Microbiol 43: 21–27.


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Copyright Info: 2016, Amal Kanti Paul, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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