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Development, characterization and shelf-life testing of a novel pulse-based snack bar

1 Department of Agricultural and Food Sciences, Faculty of Agriculture, University of Mauritius, Mauritius
2 Department of Agricultural Production Systems, Faculty of Agriculture, University of Mauritius, Mauritius
3 Department of Health Sciences, Faculty of Science, University of Mauritius, Mauritius

With the globalization of food trade, we are observing tremendous changes in eating patterns of youngsters. Snack bars represent convenient, appropriately portioned, Ready-To-Eat food items, which not only supply nutrients to the body but also provide a feeling of satiety. The aim of this study was to formulate a novel high-protein, low Glycemic Index and low-fat snack bar that can be eaten on-the-move. Twelve different pulse-based bar formulations were developed and 85.1% of sensory panelists indicated that they particularly liked the taste of formulation M1. Since M1 contained peanuts, a nut-free and date-free equivalent (mM1) was developed to cater for individuals with allergies to these ingredients. A dehydrated mix (DM) based on the mM1 composition, was also developed. The microbiological and sensorial shelf lives of the products were then determined during storage at either ambient (ca. 23 ℃) or refrigerated temperatures (ca. 4 ℃) by determining counts of aerobic bacteria and yeast and mold. Mean aerobic bacteria and yeast and mold counts of M1 fell in the range of 8.4–9.4 and 4.5–5.4 log cfu/g and 7.5–8.6 and 3.8–4.9 log cfu/g during storage at room and refrigerated temperatures respectively. Aerobic bacteria and yeast and mold counts were consistently higher under ambient storage. Since a microbial population density >7 Log CFU/g usually marks the onset of microbiological spoilage, the bars were estimated to have a microbiological shelf-life of <2 days. Overall this study points to the development of a tasty and nutritious pulse-based Ready-To-Eat snack as well as a dehydrated mix that can be readily reconstituted at home or at work.
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Keywords snack bar; pulses; sensory; microbiology; shelf-life

Citation: Tina Bhakha, Brinda Ramasawmy, Zaynab Toorabally, Hudaa Neetoo. Development, characterization and shelf-life testing of a novel pulse-based snack bar. AIMS Agriculture and Food, 2019, 4(3): 756-777. doi: 10.3934/agrfood.2019.3.756


  • 1. Sharma C, Kaur A, Aggarwal P, et al. (2014) Cereal bars-a healthful choice a review.Carpathian J Food Sci Technol 6: 29–36.
  • 2. Ranjana S, Mahomoodally F, Ramasawmy D (2013) Is healthy eating behaviour common among school adolescents in Mauritius? Food Nutr J Org. Available from: http://www.foodandnutritionjournal.org/volume1number1/is-healthy-eating-behaviour common-among-school-adolescents-in-mauritius/.
  • 3. Crofton EC, Markey A, Scannell AGM (2013) Consumers' expectations and needs towards healthy cereal based snacks. Br Food J 115: 1130–1148.    
  • 4. Green H, Siwajek P, Roulin A (2017) Use of nutrient profiling to identify healthy versus unhealthy snack foods and whether they can be part of a healthy menu plan. J Nutr Intermediary Metab 9: 1–5.
  • 5. Wate JT, Snowdon W, Millar L, et al. (2013) Adolescent dietary patterns in Fiji and their relationships with standardized body mass index. Int J Behav Nutr Phys Activity 9: 45.
  • 6. Bellisle F (2014) Meals and snacking, diet quality and energy balance. Physiol Behav 134: 38–43.    
  • 7. McIntyre C, Baid A (2009) Indulgent snack experience attributes and healthy choice alternatives. Br Food J 111: 486–497. Available from: http://dx.doi.org/10.1108/00070700910957311.    
  • 8. Gray J, Armstrong G, Farley H (2003) Opportunities and constraints in the functional food market. Nutr Food Sci 33: 213–218.    
  • 9. Scott-Thomas C (2009) Whole grain sales set to rise on sound nutritional science. Available from: https://www.foodnavigator-usa.com/Article/2009/04/02/Whole-grain-sales-set-to-rise-on-sound-nutritional-science (Accessed 14 July 2019).
  • 10. Aleksejeva S, Siksna I, Rinkule S (2017) Composition of Cereal Bars. J Health Sci 5. Available from: http://dx.doi.org/10.17265/2328-7136/2017.03.004.
  • 11. Njike VY, Smith TM, Shuval O, et al. (2016) Snack Food, Satiety, and Weight. Adv Nutr 7: 866–878. Available from: http://dx.doi.org/10.3945/an.115.009340.    
  • 12. Iqbal A, Khalil IA, Ateeq N, et al. (2006) Nutritional quality of important food legumes. Food Chem 97: 331–335. Available from: http://dx.doi.org/10.1016/j.foodchem.2005.05.011.    
  • 13. Ryland D (2003) Development of a nutritious acceptable snack bar using micronized flaked lentils. MSc Thesis, University of Manitoba.
  • 14. Jiang YZ, Wang T (2005) Phytosterols in cereal by-products. J Am Oil Chem Soc 82: 439–444.    
  • 15. Kopsell DA, Armel GR, Mueller TC, et al. (2009) Increase in nutritionally important sweet corn kernel carotenoids following mesotrione and atrazine applications. J Agr Food Chem 57: 6362–6368. Available from: http://dx.doi.org/10.1021/jf9013313.    
  • 16. Clifford T, Howatson G, West D, et al. (2015) The potential benefits of red beetroot supplementation in health and disease. Nutrients 7: 2801–2822. Available from: http://dx.doi.org/10.3390/nu7042801.    
  • 17. Tanumihardjo SA, Suri D, Simon P, et al. (2016) Vegetables of temperate climates: Carrot, parsnip, and beetroot. Encycl Food Health 387–392. Available from: http://dx.doi.org/10.1016/b978-0-12-384947-2.00714-5.
  • 18. Damasceno K, Gonçalves ACA, Pereira DSG, et al. (2016) Development of cereal bars containing pineapple peel flour (Ananas comosus L. Merril) J Food Qual 39: 417–424. Available from: http://dx.doi.org/10.1111/jfq.12222.    
  • 19. AOAC (17th eds) (2000) Official methods of analysis of AOAC International, Gaithersburg. MD, USA: Association of Official Analytical Chemists (AOAC) International.
  • 20. Vanderzant C, Splittstosser D (1992) Compendium of methods for the microbiological examination of foods, 3 Eds., American Public Health Association, Washington, USA.
  • 21. Wigmann EF, Saccomori F, Bernardi AO, et al. (2015) Toxigenic penicillia spoiling frozen chicken nuggets. Food Res Int 67: 219–222.    
  • 22. Monnet AF, Laleg K, Michon C, et al. (2019) Legume-enriched cereal products: A generic approach derived from material science to predict their structuring by the process and their final properties. Trends Food Sci Technol 86: 131–143.    
  • 23. Pellett PL, Young VR (1988) Commentary: Protein and amino acid needs for adults. Ecol Food Nut 21: 321–330. Available from: http://dx.doi.org/10.1080/03670244.1988.9991046.    
  • 24. Khederoo D (2018) Split Peas Health Benefits. Naturalfoodbenefits.com. Available from: http://www.naturalfoodbenefits.com/list-details.php?CAT=6&ID=107.
  • 25. Rafiu A, Hassan CZ, Arifin N, et al. (2015) Physico-chemical analysis of high fibre cereal bar formulated with glutinous rice flakes and sunnah foods. J Adv Food Sci Technol 2: 47–58.
  • 26. Shi L, Arntfield SD, Nickerson M (2018) Changes in levels of phytic acid, lectins and oxalates during soaking and cooking of Canadian pulses. Food Res Int 107: 660–668.    
  • 27. Torres ER, Castro ES, Santana RF, et al. (2011) Cereal bar development using exotic fruit. Proceedings of 11th ICEF Conference on Engineering and Food, Food Process Engineering in a Changing World, Athens, Greece.
  • 28. Bureau S, Mouhoubi S, Touloumet L, et al. (2015) Are folates, carotenoids and vitamin C affected by cooking? Four domestic procedures are compared on a large diversity of frozen vegetables. LWT-Food Sci Technol 64: 735–741.
  • 29. Ravi R, Ajila CM, Rao UJSP (2011) Role of steaming and toasting on the odor, protein characteristics of Chickpea (Cicer arietinum L.) flour, and product quality. J Food Sci 76: S148–S155. Available from: http://dx.doi.org/10.1111/j.1750-3841.2010.01977.x.
  • 30. Bach V, Mikkelsen L, Kidmose U, et al. (2015) Culinary preparation of beetroot (Beta vulgaris L.): The impact on sensory quality and appropriateness. J Sci Food Agr 95: 1852–1859.
  • 31. Bhattacharya S (2014) Roasting and toasting operations in food: Process engineering and applications. Available from: https://onlinelibrary.wiley.com/doi/pdf/10.1002/9781118406281.ch10.
  • 32. Olsen A, Ritz C, Kramer L, et al. (2012) Serving styles of raw snack vegetables. What do children want? Appetite 59: 556–562.
  • 33. Deshmukh GP, Inka P, Sindhav R, et al. (2010) Application of beetroot as natural coloring pigment and functional ingredient in dairy and food products. Int J Curr Microbiol Appl Sci 7: 2010–2016. Available from: http://dx.doi.org/10.20546/ijcmas.2018.712.231.
  • 34. ACAAI Public Website (2014) Peanut Allergy. Available from: https://acaai.org/allergies/types/food-allergies/types-food-allergy/peanut-allergy.
  • 35. Kwaasi AA, Harfi HA, Parhar RS, et al. (1999) Allergy to date fruits: Characterization of antigens and allergens of fruits of the date palm (Phoenix dactylifera L.). Pub Med NCBI. Available from: https://www.ncbi.nlm.nih.gov/pubmed/10688430.
  • 36. NPD Group (2011) How many people skip breakfast. Available from: https://www.npd.com/wps/portal/npd/us/news/press-releases/pr_111011b/.
  • 37. Munhoz CL, Guimarães R de CA, Nozaki VT, et al. (2014) Preparation of a cereal bar containing bocaiuva: Physical, nutritional, microbiological and sensory evaluation. Acta Sci Technol 36: 553. Available from: http://dx.doi.org/10.4025/actascitechnol.v36i3.18561.    
  • 38. Da Silva EP, Siqueira HH, do Lago RC, et al. (2013) Developing fruit-based nutritious snack bars. J Sci Food Agric 94: 52–56. Available from: http://dx.doi.org/10.1002/jsfa.6282.
  • 39. Rolls BJ (2009) Sensory-specific satiety. Nutr Rev 44: 93–101. Available from: http://dx.doi.org/10.1111/j.1753-4887.1986.tb07593.x.    
  • 40. Almiron-Roig E, Flores SY, Drewnowski A (2004) No difference in satiety or in subsequent energy intakes between a beverage and a solid food. Physiol Behav 82: 671–677. Available from: http://dx.doi.org/10.1016/j.physbeh.2004.06.003.    
  • 41. Rolls E, Rolls B, Rowe E (1983) Sensory-specific and motivation-specific satiety for the sight and taste of food and water in man. Physiology Behavior 30: 185–192. Available from: http://dx.doi.org/10.1016/0031-9384(83)90003-3.    
  • 42. Ezeokeke CT, Onuoha AB (2016) Nutrient composition of cereal (maize), legume (soybean) and fruit (banana) as a complementary food for older infants and their sensory assessment. J Food Sci Eng 6: 139–148.
  • 43. Nielsen N (2006) Development and sensory evaluation of snack bars with bean-based filling. Cereal Foods World. Available from: http://dx.doi.org/10.1094/cfw-51-0313.
  • 44. Ndb.nal.usda.gov (2016) Food composition databases show foods-Peanuts, all types, raw. Available from: https://ndb.nal.usda.gov/ndb/foods/show/4825?manu=&fgcd=&ds=Standard%20Reference.
  • 45. Peanut-institute.org (2018) The peanut institute-protein, fats and fiber. Available from: http://www.peanut-institute.org/health-and-nutrition/protective-nutrients/protein-fats-and-fiber.asp.
  • 46. Appel LJ, Sacks FM, Carey VJ, et al. (2005) Effects of protein, monounsaturated fat, and carbohydrate intake on blood pressure and serum lipids: Results of the OmniHeart randomized trial. JAMA 294: 2455–2464.    
  • 47. Kris-Etherton PM, Pearson TA, Wan Y, et al. (1999) High monounsaturated fatty acid diets lower both plasma cholesterol and triacylglycerol concentrations. Am J Clin Nutr 70: 1009–1015.    
  • 48. Harvard Health Publishing School (2017) The truth about fats: The good, the bad, and the in-between-Harvard health. Harv Health. Available from: https://www.health.harvard.edu/staying-healthy/the-truth-about-fats-bad-and-good.
  • 49. Weightlossresources.co.uk (2017) How many calories in alpen fruit and nut cereal bars. Available from: https://www.weightlossresources.co.uk/calories-in-food/snacks/alpen-fruit-and-nut-cereal-bars.htm.
  • 50. Padmashree A, Sharma GK, Kadaba AS, et al. (2012) Development of shelf stable protein rich composite cereal bar. J Food Sci Technol Mysore 49: 335–341.    
  • 51. Hutkins RW, Nannen NL (1993) pH Homeostasis in Lactic Acid Bacteria. Fac Publ Food Sci Technol: 28.
  • 52. Yilmaz Y, Toledo R (2005) Antioxidant activity of water-soluble Maillard reaction products. Food Chem 93: 273–278.    
  • 53. Tamanna N, Mahmood N (2015) Food processing and Maillard reaction products: Effect on humanhealth and nutrition. Int J Food Sci: 526–762.
  • 54. Loveday SM, Hindmarsh JP, Creamer LK, et al. (2009) Physicochemical changes in a model protein bar during storage. Food Res Int 42: 798–806.    
  • 55. Niyogi K, Bjorkman O, Grossman A (1997) The roles of specific xanthophylls in photoprotection. Proc Nat Acad Sci US Am 94: 14162–14167.    
  • 56. Cox NA, Russell SM, Bailey JS (1998) The microbiology of stored poultry. In: Davies A, Board R, Eds., Microbiol Meat Poult 266–287. London: Blackie Academic and Professional Press.
  • 57. Jay JM (2000) Modern food microbiology. Springer US. Available from: http://dx.doi.org/10.1007/978-1-4615-4427-2.
  • 58. Tilbury RH (1980) Xerotolerant (osmophilic) yeasts. In: Skinner FA, Passmore SM, Davenport RR, Eds., Biology Activities Yeasts, Society for Applied Bacteriology Symposium Series. London: Academic Press.
  • 59. Tokuoka K, Ishitania T, Goto S, et al. (1985) Identification of yeasts isolated from high-sugar foods. J Gen Appl Microbial 31: 411.    
  • 60. Jermini MFG, Geiges O, Schmidt-Lorenz WM (1987) Detection isolation and identification of osmotolerant yeasts from high-sugar products. J Food Protect 50: 468.    
  • 61. Abdullah N, Nawawi A, Othman I (2000) Fungal spoilage of starch-based foods in relation to its water activity (aw). J Stored Prod Res 36: 47–54. Available from: http://dx.doi.org/10.1016/s0022-474x(99)00026-0.    
  • 62. Perchonok M (2008) Shelf-Life Considerations and Techniques. Food Product Development: Based on Experience [Internet]. Iowa State Press, 59–73. Available from: http://dx.doi.org/10.1002/9780470376898.ch6.
  • 63. IFST (1993) Shelf life of foods–guidelines for its determination and prediction. IFST, London.
  • 64. Simon ML, Jason PH, Lawrence KC, et al. (2009) Physicochemical changes in a model protein bar during storage. Food Res Int 42: 798–80.    


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