Research article

Soil management practices affect arbuscular mycorrhizal fungi propagules, root colonization and growth of rainfed maize

  • Received: 16 April 2018 Accepted: 14 June 2018 Published: 25 June 2018
  • Agronomic management practices influence beneficial soil biota, especially arbuscular mycorrhizal fungi (AMF). AMF colonizes about eighty percent of land plants, promoting absorption of essential nutrients and crop growth. Here, a 5-year field experiment was carried out in Central Kenyan Highlands to determine the effect of tillage, mulching and inorganic fertilizers on the number of infective AMF propagules in the soil, mycorrhizal root colonization of maize and uptake of P and N from the soil. The study involved conventional and minimum tillage systems, mulching using dried maize stovers and inorganic fertilizers (120 kg N/ha). The experiment was set up in randomized complete block design and replicated thrice. The number of infective AMF propagules decreased in the following order; V4 stage (p < 0.0001), V6 stage (p < 0.0001), maize harvest (p = 0.0076) and before maize planting (p = 0.0061). Minimum tillage + mulch + no NP fertilizer (ZRO) treatment recorded the highest number of infective AMF propagules with an average of 90 propagules g-1 of soil whereas conventional tillage + mulch + NP fertilizer (CRF) and conventional tillage + no mulch + NP fertilizer (CWF) treatments recorded the lowest number of AMF propagules with an average of 1.33 propagules g-1 of soil. Besides, AMF colonization of maize roots at V4, V6 and harvest stages was significantly affected by tillage (p < 0.0001), mulch (p = 0.0001) and fertilizer (p < 0.0001). Results at juvenile stage showed a strong positive correlation between AMF colonization and shoot P (r = 0.933, p < 0.0001) and N (r = 0.928, p < 0.0001). These findings demonstrate a strong effect of agronomic management practices on soil AMF propagules which subsequently affected root colonization and uptake of essential nutrients such as P and N.

    Citation: Methuselah Mang’erere Nyamwange, Ezekiel Mugendi Njeru, Monicah Mucheru-Muna, Felix Ngetich. Soil management practices affect arbuscular mycorrhizal fungi propagules, root colonization and growth of rainfed maize[J]. AIMS Agriculture and Food, 2018, 3(2): 120-134. doi: 10.3934/agrfood.2018.2.120

    Related Papers:

  • Agronomic management practices influence beneficial soil biota, especially arbuscular mycorrhizal fungi (AMF). AMF colonizes about eighty percent of land plants, promoting absorption of essential nutrients and crop growth. Here, a 5-year field experiment was carried out in Central Kenyan Highlands to determine the effect of tillage, mulching and inorganic fertilizers on the number of infective AMF propagules in the soil, mycorrhizal root colonization of maize and uptake of P and N from the soil. The study involved conventional and minimum tillage systems, mulching using dried maize stovers and inorganic fertilizers (120 kg N/ha). The experiment was set up in randomized complete block design and replicated thrice. The number of infective AMF propagules decreased in the following order; V4 stage (p < 0.0001), V6 stage (p < 0.0001), maize harvest (p = 0.0076) and before maize planting (p = 0.0061). Minimum tillage + mulch + no NP fertilizer (ZRO) treatment recorded the highest number of infective AMF propagules with an average of 90 propagules g-1 of soil whereas conventional tillage + mulch + NP fertilizer (CRF) and conventional tillage + no mulch + NP fertilizer (CWF) treatments recorded the lowest number of AMF propagules with an average of 1.33 propagules g-1 of soil. Besides, AMF colonization of maize roots at V4, V6 and harvest stages was significantly affected by tillage (p < 0.0001), mulch (p = 0.0001) and fertilizer (p < 0.0001). Results at juvenile stage showed a strong positive correlation between AMF colonization and shoot P (r = 0.933, p < 0.0001) and N (r = 0.928, p < 0.0001). These findings demonstrate a strong effect of agronomic management practices on soil AMF propagules which subsequently affected root colonization and uptake of essential nutrients such as P and N.


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    [1] Turrini A, Sbrana C, Avio L, et al. (2016) Changes in the composition of native root arbuscular mycorrhizal fungal communities during a short-term cover crop-maize. Biol Fert Soils 52: 642–653.
    [2] Njeru i, Bocci G, Avio L, et al. (2017) Functional identity has a stronger effect than diversity on mycorrhizal symbiosis and productivity of field grown organic tomato. Eur J Agron 86: 1–11. doi: 10.1016/j.eja.2017.02.007
    [3] Mader P, Edenhofer S, Boller T, et al. (2000) Arbuscular mycorrhizae in a long-term field trial comparing low-input (organic, biological) and high-input (conventional) farming systis in a crop rotation. Biol Fert Soils 31: 150–156. doi: 10.1007/s003740050638
    [4] Cavagnaro TR, Jackson LE, Six J, et al. (2005) Arbuscular mycorrhizas, microbial communities, nutrient availability, and soil aggregates in organic tomato production. Plant Soil 282: 209–225.
    [5] Verzeaux J, Nivelle E, Roger D, et al. (2017) Spore density of arbuscular mycorrhizal fungi is fostered by six years of a no-till systi and is correlated with environmental parameters in a silty loam soil. Agron 7: 38. doi: 10.3390/agronomy7020038
    [6] Njeru i, Avio L, Sbrana C, et al. (2014) First evidence for a major cover crop effect on arbuscular mycorrhizal fungi and organic maize growth. Agron Sustain Dev 34: 841–848. doi: 10.1007/s13593-013-0197-y
    [7] Borie F, Rubio R, Rouanet JL, et al. (2006) Effects of tillage systis on soil characteristics, glomalin and mycorrhizal propagules in a Chile Ultisol. Soil Tillage Res 88: 253–261. doi: 10.1016/j.still.2005.06.004
    [8] Nyaga J, Jefwa JM, Muthuri CW, et al. (2015) Arbuscular mycorrhizal fungi with different soil fertility amendment practices in agricultural landscapes of Kenyan Highlands. Nutr Cycl Agroecosys 103: 229–240. doi: 10.1007/s10705-015-9744-z
    [9] Martinez TN and Johnson NC (2010) Agricultural managient influences propagule densities and functioning of arbuscular mycorrhizas in low- and high- input agroecosystis in arid environments. Appl Soil Ecol 46: 300–306. doi: 10.1016/j.apsoil.2010.07.001
    [10] Wang FY, Hu JL, Lin XG, et al. (2011) Arbuscular mycorrhizal fungal community structure and diversity in response to long-term fertilization: a field case from China. World J Microb Biot 27: 67–74. doi: 10.1007/s11274-010-0427-2
    [11] Alguacil MM, Lumini E, Roldan A, et al. (2008) The impact of tillage practices on arbuscular mycorrhizal fungal diversity in subtropical crops. Ecol Appl 18: 527–536. doi: 10.1890/07-0521.1
    [12] Kabir Z (2005) Tillage or no-tillage: Impact on mycorrhizae. Can J Plant Sci 85: 23–29. doi: 10.4141/P03-160
    [13] Oruru MB and Njeru i (2016) Upscaling arbuscular mycorrhizal symbiosis and related agroecosystis services in smallholder farming systis. Biomed Res Int 2016: 4376240.
    [14] Verbruggen E, Roling WFM, Gamper HA, et al. (2010) Positive effects of organic farming on below-ground mutualists: large-scale comparison of mycorrhizal fungal communities in agricultural soils. New Phytol 186: 968–979. doi: 10.1111/j.1469-8137.2010.03230.x
    [15] Roldan A, Salinas-Garcia JR, Alguacil M, et al. (2007) Changes in soil sustainability indicators following conservation tillage practices under subtropical maize and bean crop. Soil Tillage Res 93: 273–282. doi: 10.1016/j.still.2006.05.001
    [16] Bedini S, Avio L, Sbrana C, et al. (2013) Mycorrhizal activity and diversity in a long-term organic Mediterranean agroecosysti. Biol Fert Soils 49: 781–790. doi: 10.1007/s00374-012-0770-6
    [17] Jaetzold R, Schmidt H, Homet ZB, et al. (2007)Farm managient handbook of Kenya: Natural conditions and farm information (2nd ed., Vol. 11/C). Nairobi: Eastern Province, Ministry of Agriculture/ GTZ.
    [18] Mugwe J, Mugendi D, Mucheru-Muna M, et al. (2009) Determinants of the decision to adopt intergrated soil fertility managient practices by smallholder farmers in Central highlanda of Kenya. Exp Agr 45: 61–75. doi: 10.1017/S0014479708007072
    [19] Mburu SW, Koskey G, Kimiti JM, et al. (2016) Agrobiodiversity conservation enhances food security in subsistence-based farming systis of Eastern Kenya. Agriculture and Food Security 5: 19. doi: 10.1186/s40066-016-0068-2
    [20] Lehman RM, Taheri WI, Osborne SL, et al. (2012) Fall cover cropping can increase arbuscular mycorrhizae in soils supporting intensive agricultural production. Appl Soil Ecol 61: 300–304. doi: 10.1016/j.apsoil.2011.11.008
    [21] Porter W (1979) The 'most probable number method' for enumerating infective propagules of vesicular arbuscular mycorrhizal fungi in soil. Aust J Soil Res 17: 515–519. doi: 10.1071/SR9790515
    [22] Mohidin H, Hanafi M, Rafii Y, et al. (2015) Determination of optimum levels of nitrogen, phosphorus and potassium of oil palm seedlings in solution culture. Bragantia 74: 247–254. doi: 10.1590/1678-4499.0408
    [23] Kissel ED and Sonon LS (2008) Soil test handbook for Georgia (Vol. 1).
    [24] Okalebo JR, Gathua WK and Woomer PL (2002) Laboratory methods of soil and plant analysis: a working manual second edition. Sacred Africa, Nairobi.
    [25] Phillips J and Hayman D (1970) Improved procedures for clearing roots and staining parastic and vesicular-arbuscular mycorrhizal fungi for rapid assessment of infection. Transactions of British Mycological Society 55: 158–161. doi: 10.1016/S0007-1536(70)80110-3
    [26] Giovannetti M and Mosse B (1980) An evaluation technique for measuring vesicular arbuscular mycorrhizal infection in roots. New Phytol 84: 489–500. doi: 10.1111/j.1469-8137.1980.tb04556.x
    [27] Cavaglieri L, Orlando J and Etcheverry M (2009) Rhizosphere microbial community structure at different maize plant growth stages and root locations. Microbiol Res 164: 391–399. doi: 10.1016/j.micres.2007.03.006
    [28] Johnston-Monje D, Lundberg DS, Lazarovits G, et al. (2016) Bacterial populations in juvenile maize rhizospheres originate from both seed and soil. Plant Soil 405: 337–355. doi: 10.1007/s11104-016-2826-0
    [29] Reji PM, Feng Y, Githinji L, et al. (2012) Impact of no-tillage and conventional tillage systis on soil microbial communities. Applied and Environmental Soil Science 2012: 1–10.
    [30] Kabir Z, O'Halloran IP, Fyles JW, et al. (1998) Dynamics of the mycorrhizal symbiosis of corn (Zea mays L.): effects of host physiology, tillage practice and fertilization on spatial distribution of extra-radical mycorrhizal hyphae in the field. Agricult Ecosys Environ 68: 151–163.
    [31] Oehl F, Sieverding E, Ineichen K, et al. (2003) Impact of land use intensity on species diversity of arbuscular mycorrhizal fungi in agroecosystis of Central Europe. Appl Environ Microb 69: 2816–2824. doi: 10.1128/AEM.69.5.2816-2824.2003
    [32] Nakhro N and Dkhar MS (2010) Populations and biomass carbon in paddy field soil. Agron J 9: 102–110. doi: 10.3923/ja.2010.102.110
    [33] Klikocka H, Narolski B, Klikocka O, et al. (2012) The effect of soil tillage and nitrogen fertilization on microbiological parameters of soil on which spring Triticale is grown. Pol J Environ Stud 21: 1675–1685.
    [34] Arihara J and Karasawa T (2000) Effects of previous crops on arbuscular mycorrhizal formation and growth of succeeding maize. Soil Sci Plant Nutr 46: 43–51. doi: 10.1080/00380768.2000.10408760
    [35] Mariela FP, Pavel MEI, Manuel SRL, et al. (2016) Dehydrogenase and mycorrhizal colonization: Tools for monitoring agroecosysti soil quality. Appl Soil Ecol 100: 144–153. doi: 10.1016/j.apsoil.2015.12.011
    [36] Bilalis DJ and Karamanos AJ (2010) Organic maize growth and mycorrhizal root colonization response to tillage and organic fertilization. J Sustain Agr 34: 836–849. doi: 10.1080/10440046.2010.519197
    [37] Habibzadeh Y (2015) The effects of arbuscular mycorrhizal fungi and phosphorous levels on dry matter production and root traits in cucumber (Cucumis sativulus L.). African Journal of Environmental Science and Technology 9: 67–70.
    [38] Ryan MH, Van Herwaarden AF, Angus JF, et al. (2005) Reduced growth of autumn-sown wheat in a low-P soil is associated with high colonisation of arbuscular mycorrhizal fungi. Plant Soil 270: 275–286. doi: 10.1007/s11104-004-1611-7
    [39] Adediran JA, Taiwo LB, Akande MO, et al. (2004) Application of organic and inorganic fertilizer for sustainable maize and cowpea yields in Nigeria. J Plant Nutr 27: 1163–1181.
    [40] Gosling P, Hodge A, Goodlass G, et al. (2006) Arbuscular mycorrhizal fungi and organic farming. Agricult Ecosys Environ 113: 17–35.
    [41] Hajiboland R, Aliasgharzad N and Barzeghar R (2009) Influence of arbuscular mycorrhizal fungi on uptake of Zn and P by two contrasting rice genotypes. Plant Soil Environ 55: 93–100. doi: 10.17221/319-PSE
    [42] Oruru MB, Njeru i, Pasquet R, et al. (2018) Response of a wild-type and modern cowpea cultivars to arbuscular mycorrhizal inoculation in sterilized and non-sterilized soil. J Plant Nutr 41: 90–101. doi: 10.1080/01904167.2017.1381728
    [43] Asghari HR and Cavagnaro TR (2012) Arbuscular mycorrhizas reduce nitrogen loss via leaching. Plos One 7: 1–5.
    [44] LeighJ, Hodge A and Fitter AH (2009) Arbuscular mycorrhizal fungi can transfer substantial amounts of nitrogen to their host plant from organic material. New Phytol 181: 199–207. doi: 10.1111/j.1469-8137.2008.02630.x
    [45] Veresoglou SD, Chen B and MC Rillig (2012) Arbuscular mycorrhiza and soil nitrogen cycling. Soil Biol Biochi 46: 53–62. doi: 10.1016/j.soilbio.2011.11.018
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