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Objective and Subjective Assessment of Digital Pathology Image Quality

Philips Digital Pathology Solutions 5684 PC Best, The Netherlands

Special Issues: WSI, and the observer performance and human factors involved in this application of WSI

The quality of an image produced by the Whole Slide Imaging (WSI) scanners is of critical importance for using the image in clinical diagnosis. Therefore, it is very important to monitor and ensure the quality of images. Since subjective image quality assessments by pathologists are very time-consuming, expensive and difficult to reproduce, we propose a method for objective assessment based on clinically relevant and perceptual image parameters: sharpness, contrast, brightness, uniform illumination and color separation; derived from a survey of pathologists. We developed techniques to quantify the parameters based on content-dependent absolute pixel performance and to manipulate the parameters in a predefined range resulting in images with content-independent relative quality measures. The method does not require a prior reference model. A subjective assessment of the image quality is performed involving 69 pathologists and 372 images (including 12 optimal quality images and their distorted versions per parameter at 6 different levels). To address the inter-reader variability, a representative rating is determined as a one-tailed 95% confidence interval of the mean rating. The results of the subjective assessment support the validity of the proposed objective image quality assessment method to model the readers’ perception of image quality. The subjective assessment also provides thresholds for determining the acceptable level of objective quality per parameter. The images for both the subjective and objective quality assessment are based on the HercepTestTM slides scanned by the Philips Ultra Fast Scanners, developed at Philips Digital Pathology Solutions. However, the method is applicable also to other types of slides and scanners.
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Copyright Info: © 2015, Prarthana Shrestha, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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