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AIMS Environmental Science, doi: 10.3934/environsci.2015.2.122

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The role of lipids in activated sludge floc formation

School of Biotechnology and Biomolecular Sciences, University of NSW, Sydney 2052, Australia

Activated sludge is widely used to treat municipal and industrial wastewater globally and the formation of activated sludge flocculates (flocs) underpins the ability to separate sludge from treated water. Despite the importance of activated sludge flocs to human civilization there have been precious few attempts to rationally design fit for purpose flocs using a bottom-up approach based on a solid scientific foundation. Recently we have been developing experimental models for activated sludge floc formation based on the colonization and consumption of particulate organic matter (chitin and cellulose). In this study we lay the foundation for investigation of activated sludge floc formation based on biofilm formation around spheres of the lipid glycerol trioleate (GT) that form spontaneously when GT is introduced into activated sludge incubations. Sludge biomass was observed to associate tightly with the lipid spheres. An increase in extracellular lipase activity was associated with a decrease in size of the colonized lipid spheres over a 25 day incubation. Bacterial community composition shifted from predominantly Betaproteobacteria to Alphaproteobacteria in GT treated sludge. Four activated sludge bacteria were isolated from lipid spheres and two of them were shown to produce AHL like quorum sensing signal activity, suggesting quorum sensing may play a role in lipid spheres colonization and biodegradation in activated sludge. The development of this experimental model of activated sludge floc formation lays the foundation for rational production of flocs for wastewater treatment using lipids as floc nuclei and further development of the flocculate life-cycle concept.

1. Introduction

In this paper, we consider the following equation for $u$

$-\Delta u=f(u)-\frac{1}{|\Omega|}\int_{\Omega} f(u)d\mathbf{x}$ (1.1)

under periodic boundary conditions. The domain $\Omega=\mathbb{T}^N$, the $N$-dimensional torus, with $N=2, 3$. Here $f$ is a given smooth function of $u$ for $u(\mathbf{x}) \in G \subset \mathbb{R}$.

We will prove that there exists a solution $u$ to equation (1.1) which is unique if $ |\frac{d f}{d u}(u_0) | < \frac{1}{(C_0)^2}$, where $u_0 \in G$ is a given constant and where $C_0$ is the constant from Poincarés inequality. And we will prove that the solution $u$ is not unique if $\frac{df}{du}(u_0) $ is a simple eigenvalue of $-\Delta$.

In previous related work, many researchers have studied the equation $-\Delta u=f(u)+g$. Existence of a solution $u$ to the equation $-\Delta u=f(u)+g$ has been proven for a Dirichlet boundary condition $u|_{\partial \Omega}=0$ (see, e.g., [1, 2, 5, 7]) under certain conditions on $f$ and, $g$. And existence of a solution $u$ to the equation $-\Delta, ,u=f(u)+g$ has been proven for a Neumann boundary condition $\frac{\partial u}{\partial n}|_{\partial \Omega}=h$ (see, e.g., [3, 4, 6]) under certain conditions on $f$ and $g$. We have not seen any work by other researchers on the existence of a solution $u$ to equation (1.1) under periodic boundary conditions. And we have not seen any work by other researchers which contains the particular condition that $ |\frac{d f}{d u}(u_0) | < \frac{1}{(C_0)^2}$, where $C_0$ is the constant from Poincarés inequality and where $u_0$ is a given constant in the domain of the function $\frac{d f}{d u}$.


2. Existence theorem

In the proof that follows, we use the standard notation for the $L^2(\Omega)$ norm of a function $g$, namely, $\|g\|_0^2=\int_\Omega | g|^2d \mathbf{x}$. And we denote the inner product as $(g, h)=\int_\Omega gh d \mathbf{x}$. Also, we let $Du$ denote the gradient of a function $u$. We also use the notation $ |\frac{df}{du} |_{0, \overline{G}_1}=\max\{|\frac{df}{du}(u_{*}) |: u_{*} \in \overline{G}_1\}$, where $\frac{df}{du}$ is a function of $u$ and where $ \overline{G}_1 \subset \mathbb{R}$ is a closed bounded interval.

The purpose of this article is to prove the following theorem.

Theorem 2.1. Consider the following equation for $u$

$-\Delta u=f(u)-\frac{1}{|\Omega|}\int_{\Omega} f(u)d\mathbf{x}$ (2.1)

where the domain $\Omega=\mathbb{T}^N$, the $N$-dimensional torus, with $N=2$ or $N=3$, and where $f$ is a given smooth function of $u$ for $u(\mathbf{x}) \in G \subset \mathbb{R}$. Let $u_0 \in G$ be a given constant. Then we have the following two cases:

(1) If $ |\frac{d f}{d u}(u_0) | < \frac{1}{(C_0)^2}$, where $C_0$ is the constant from Poincarés inequality, then there exists a unique classical solution $u(\mathbf{x})\in \overline{G}_1$ to equation (2.1) which satisfies the condition $u(\mathbf{x}_0)=u_0$, where $\overline{G}_1 \subset G \subset \mathbb{R}$ and where $u_0 \in \overline{G}_1$ and where $\mathbf{x}_0 \in \Omega$ is a given point. This unique classical solution is $u=u_0$.

(2) If $\frac{df}{du} (u_0) $ is a simple eigenvalue of $-\Delta$ then there exists a solution $u$ of equation (2.1) which is not the constant function $u_0$. This solution $u$ may not necessarily satisfy the condition $u(\mathbf{x}_0)=u_0$. }

Proof.

We will consider separately each of the two cases from the statement of the theorem. First, we will consider Case 1 from the statement of Theorem 2.1

Suppose that $ |\frac{d f}{d u}(u_0) | < \frac{1}{(C_0)^2}$, where $C_0$ is the constant from Poincarés inequality and where $u_0 \in G$ is a given constant. It follows that there exists a closed bounded interval $\overline{G}_1 \subset G$ such that $u_0 \in \overline{G}_1$ and such that $|\frac{d f}{d u} |_{0, \overline{G}_1} < \frac{1}{(C_{0})^2}$, where $|\frac{d f}{d u} |_{0, \overline{G}_1}=\max\{|\frac{d f}{d u} (u_{*}) |:u_{*} \in \overline{G}_1\}$. Suppose that $u$ is a classical solution of equation (2.1) such that $u(\mathbf{x}) \in \overline{G}_1$ for all $ \mathbf{x} \in \Omega$ and $u$ satisfies the condition $u(\mathbf{x}_0)=u_0$, where $\mathbf{x}_0 \in \Omega$ is a given point. We will now prove that this solution is $u=u_0$.

From equation (2.1), and from using integration by parts and Poincarés inequality, we obtain the following estimate for $ \Vert D u\Vert_{0}^2$:

$\begin{gathered} \left\| {Du} \right\|_0^2=(-\Delta u, u-\frac{1}{{|\Omega |}}\int_\Omega u d{\mathbf{x}}) \\=(f(u)-\frac{1}{{|\Omega |}}\int_\Omega f (u)d{\mathbf{x}}, u-\frac{1}{{|\Omega |}}\int_\Omega u d{\mathbf{x}}) \\ \leq {\left\| {f(u)-\frac{1}{{|\Omega |}}\int_\Omega f (u)d{\mathbf{x}}} \right\|_0}{\left\| {u-\frac{1}{{|\Omega |}}\int_\Omega u d{\mathbf{x}}} \right\|_0} \\ \leq {({C_0})^2}{\left\| {Df(u)} \right\|_0}{\left\| {Du} \right\|_0} \\ \end{gathered} $ (2.2)

where we used Poincarés inequality to obtain $\|u-\frac{1}{|\Omega|}\int_{\Omega} u d\mathbf{x} \|_0 \leq C_0 \|D u \|_0$ and $\|f(u)-\frac{1}{|\Omega|}\int_{\Omega} f(u)d\mathbf{x} \|_0 \leq C_0 \|Df(u) \|_0$.

From (2.2) we obtain the inequality

$\begin{gathered} \left\| {Du} \right\|_0^2 \leq {({C_0})^4}\left\| {Df(u)} \right\|_0^2 \\ \leq {({C_0})^4}|\frac{{df}}{{du}}|_{{L^\infty }(\Omega)}^2\left\| {Du} \right\|_0^2 \\ \leq {({C_0})^4}|\frac{{df}}{{du}}|_{0, {{\bar G}_1}}^2\left\| {Du} \right\|_0^2 \\ \end{gathered} $ (2.3)

where we used the assumption that $u(\mathbf{x})\in \overline{G}_1$ for all $\mathbf{x}\in \Omega$, and so it follows that $|\frac{d f}{d u} |_{L^{\infty}(\Omega)}\leq |\frac{d f}{d u} |_{0, \overline{G}_1}$, where $|\frac{d f}{d u} |_{0, \overline{G}_1}=\max\{|\frac{d f}{d u} (u_{*}) |:u_{*} \in \overline{G}_1\}$.

Since $ \Big|\frac{d f}{du} \Big|_{0, \overline{G}_1}^2 < \frac{1}{(C_0)^4}$, it follows from (2.3) that $\Vert D u\Vert_{0}=0$ and so the solution $u$ of equation (2.1) is a constant. Therefore the solution $u=u_0$ is the unique classical solution of equation (2.1) in $\overline{G}_1$ which satisfies the condition $u(\mathbf{x}_0)=u_0$. This completes the proof of Case 1 in the statement of Theorem 2.1.

Next, we consider Case 2 in the statement of Theorem 2.1. We now prove that if $\frac{d f}{du} (u_0) $ is a simple eigenvalue of $-\Delta$ then there exists a solution $u$ of equation (2.1) which is not the constant solution $u_0$. We remark that this solution $u$ may not necessarily satisfy the condition that $u(\mathbf{x}_0)=u_0$, where $\mathbf{x}_0 \in \Omega$ is a given point.

We begin by letting $v=u-u_0$ and write equation (2.1) equivalently as

$\begin{gathered}-\Delta v=-\Delta u=f(u)-\frac{1}{{|\Omega |}}\int_\Omega f (u)d{\mathbf{x}} \\=(f(u)-f({u_0}))-\frac{1}{{|\Omega |}}\int_\Omega {(f(} u)-f({u_0}))d{\mathbf{x}} \\=(\frac{{df}}{{du}}({u_0} + {t_1}(u-{u_0})))(u-{u_0})-\frac{1}{{|\Omega |}}\int_\Omega (\frac{{df}}{{du}}({u_0} + {t_1}(u-{u_0})))(u-{u_0})d{\mathbf{x}} \\=(\frac{{df}}{{du}}({u_0} + {t_1}v))v-\frac{1}{{|\Omega |}}\int_\Omega (\frac{{df}}{{du}}({u_0} + {t_1}v))vd{\mathbf{x}} \\ \end{gathered} $ (2.4)

where $t_{1} \in (0, 1)$. Here we used the mean value theorem.

We next obtain the identity

$\begin{gathered} \frac{{df}}{{du}}({u_0} + {t_1}v)=\frac{{df}}{{du}}({u_0} + {t_1}v)-\frac{{df}}{{du}}({u_0}) + \frac{{df}}{{du}}({u_0}) \\=(\frac{{{d^2}f}}{{d{u^2}}}({u_0} + {t_2}({t_1}v))){t_1}v + \frac{{df}}{{du}}({u_0}) \\ \end{gathered} $ (2.5)

where $t_{2} \in (0, 1)$. And we again used the mean value theorem.

Substituting (2.5) into (2.4) yields

$-\Delta v=\frac{{df}}{{du}}({u_0})v + (\frac{{{d^2}f}}{{d{u^2}}}({u_0} + {t_2}({t_1}v))){t_1}{v^2}-\frac{1}{{|\Omega |}}\int_\Omega (\frac{{df}}{{du}}({u_0} + {t_1}v))vd{\mathbf{x}}$ (2.6)

where $v=u-u_0$, where $t_{1} \in (0, 1)$, and where $t_{2} \in (0, 1)$.

We can write equation (2.6) in the form

$\Delta v+ \lambda v=g(v)$ (2.7)

where $\lambda=\frac{d f}{du}(u_{0})$ and where $g(v)=-\Big(\frac{d^2 f}{du^2}(u_0+t_{2}(t_{1}v))\Big)t_{1}v^2 $ $+\frac{1}{|\Omega|}\int_{\Omega}\Big(\frac{d f}{du}(u_{0}+t_{1}v)\Big) v d\mathbf{x}$.

Let $F(v, \lambda)=\Delta v+ \lambda v-g(v) $. We will apply the the implicit function theorem to the equation $F(v, \lambda)=0$. Note that $g(0)=0$ and $g^{\prime}(0)=0 $.

If $\lambda=\frac{d f}{du}(u_{0})$ is not an eigenvalue of $-\Delta$, it follows from the implicit function theorem that $v=0$ is the only small solution to the equation $F(v, \lambda)=0$ when $F(v, \lambda)=\Delta v+ \lambda v-g(v) $ and when $g(0)=0$ and $g^{\prime}(0)=0 $ (see, e.g., [7]). Therefore $u=u_0$ is the only solution of equation (2.1) in a neighborhood of $u_0$.

If $\lambda=\frac{d f}{du}(u_{0})$ is a simple eigenvalue of $-\Delta$, it follows from the implicit function theorem that there exists a non-trivial solution $v$ to the equation $F(v, \lambda)=0$ when $F(v, \lambda)=\Delta v+ \lambda v-g(v) $ and when $g(0)=0$ and $g^{\prime}(0)=0 $ (see, e.g., [7]). Therefore there exists a solution $u$ to equation (2.1) which is not the constant function $u_0$.

This completes the proof of Theorem 2.1.


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