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Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment

1 Sealy Center for Structural Biology and Molecular Biophysics, University of Texas Medical Branch, Galveston, Texas, USA;
2 Department of Biochemistry and Molecular Biology, University of Texas Medical Branch, Galveston, Texas, USA;
3 Institute for Human Infections and Immunity, University of Texas Medical Branch, Galveston, Texas, USA;
4 Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, USA;
5 JEOL USA, Inc., Peabody, Massachusetts, USA;
6 Direct Electron, LP, San Diego, California, USA;
7 E. A. Fischione Instruments, Inc., Export, Pennsylvania, USA;
8 Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, Texas, USA

Special Issue: Structural analysis of macromolecules using Cryo electron microscopy

A unique biological safety level (BSL)-3 cryo-electron microscopy facility with a 200 keV high-end cryo-electron microscope has been commissioned at the University of Texas Medical Branch (UTMB) to study the structure of viruses and bacteria classified as select agents. We developed a microscope decontamination protocol based on chlorine dioxide gas with a continuous flow system. In this paper we report on testing digital camera sensors (both CCD and CMOS direct detector) in a BSL-3 environment, and microscope performance after chlorine dioxide (ClO2) decontamination cycles.
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Keywords cryo-electron microscopy; single particle imaging; biological safety containment

Citation: Michael B. Sherman, Juan Trujillo, Benjamin E. Bammes, Liang Jin, Matthias W. Stumpf, Scott C. Weaver. Decontamination of digital image sensors and assessment of electron microscope performance in a BSL-3 containment. AIMS Biophysics, 2015, 2(2): 153-162. doi: 10.3934/biophy.2015.2.153

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Copyright Info: 2015, Michael B. Sherman, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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