Research article

Microwave processing of sunflower achenes and its influence on their quality and enzymes activities

  • Received: 11 September 2020 Accepted: 19 November 2020 Published: 08 December 2020
  • Stricter requirements to environmental compatibility and smaller energy-output ratio highlight the importance of implementing the super high-frequency drying of the crop seeds. The study is aimed at the development of drying optimal parameters and modes of sunflower (Helianthus annuus L.) seeds in a settled small-size conveyor microwave unit. In-gel activity of 6 functionally key enzymes (formate dehydrogenase, glutamate dehydrogenase, malate dehydrogenase, diaphorase, leucine aminopeptidase and non-specific esterases) in polyacrylamide gels after disk electrophoresis was analyzed in order to count on an additional assessment of the temperature influence caused by electromagnetic radiation of the tested drying unit on the sunflower achenes metabolism. The correlation analysis showed the existence of the statistically significant (р < 0.05) negative dependence between the seed materials heating temperature with germination energy (correlation coefficient −0.783) and achenes germination (−0.797). These two parameters (without processing 88 and 96%, respectively) began to reduce sharply when reaching the heating temperatures of 55℃ and more. Enzymes de-activation also started within this range. Considering the collected data about drying of the seed material, the optimal heating conditions were within 26–27 minutes at 800 W and heating temperature 38–40° С. With these parameters the quality of the processed seeds was preserved, and the costs for drying were relatively low (2.61 MJ per 1 kg of the water removed).

    Citation: Ilgam Masalimov, Shamil Fayzrakhmanov, Yulai Yanbaev, Albina Tagirova, Zagir Kiyamov. Microwave processing of sunflower achenes and its influence on their quality and enzymes activities[J]. Mathematical Biosciences and Engineering, 2021, 18(1): 445-455. doi: 10.3934/mbe.2021024

    Related Papers:

  • Stricter requirements to environmental compatibility and smaller energy-output ratio highlight the importance of implementing the super high-frequency drying of the crop seeds. The study is aimed at the development of drying optimal parameters and modes of sunflower (Helianthus annuus L.) seeds in a settled small-size conveyor microwave unit. In-gel activity of 6 functionally key enzymes (formate dehydrogenase, glutamate dehydrogenase, malate dehydrogenase, diaphorase, leucine aminopeptidase and non-specific esterases) in polyacrylamide gels after disk electrophoresis was analyzed in order to count on an additional assessment of the temperature influence caused by electromagnetic radiation of the tested drying unit on the sunflower achenes metabolism. The correlation analysis showed the existence of the statistically significant (р < 0.05) negative dependence between the seed materials heating temperature with germination energy (correlation coefficient −0.783) and achenes germination (−0.797). These two parameters (without processing 88 and 96%, respectively) began to reduce sharply when reaching the heating temperatures of 55℃ and more. Enzymes de-activation also started within this range. Considering the collected data about drying of the seed material, the optimal heating conditions were within 26–27 minutes at 800 W and heating temperature 38–40° С. With these parameters the quality of the processed seeds was preserved, and the costs for drying were relatively low (2.61 MJ per 1 kg of the water removed).



    加载中


    [1] W. H. Morrison, J. A. Robertson, Effects of drying on sunflower seed oil quality and germination, J. Am. Oil Chem. Soc., 55 (1978), 272–274. doi: 10.1007/BF02676940
    [2] H. Darvishi, M. Hadi Khoshtaghaza, G. Najafi, M. Zarein, Characteristics of sunflower seed drying and microwave energy consumption, Int. Agrophys., 27 (2013), 127–132. doi: 10.2478/v10247-012-0077-8
    [3] A. V. Bastron, A. V. Zapletina, A. V. Logachev, Overview of the microwave installations for the pre-sowing treatment of the agricultural crop seeds, Bull. KrasGAU, 5 (2015), 63–68.
    [4] G. J. Seiler, T. J. Gulya, Sunflower: Overview, in Encyclopedia of Food Grains, (eds. C. W. Wrigley, H. Corke, K. Seetharaman, J. Faubion, ), Elsevier, 2016,247–253.
    [5] T. A. de Souza Smaniotto, O. Resende, K. A. de Sousa, R. C. Campos, D. N. Guimarães, G. B. Rodrigues, Physical properties of sunflower seeds during drying, Semin. Ciências Agrárias, 38 (2017), 157–164. doi: 10.5433/1679-0359.2017v38n1p157
    [6] S. M. D. H. Jayasanka, T. Asaeda, The significance of microwaves in the environment and its effect on plants, Environ. Rev., 22 (2014), 220–228. doi: 10.1139/er-2013-0061
    [7] S. D. S. Araújo, S. Paparella, D. Dondi, A. Bentivoglio, D. Carbonera, A. Balestrazzi, Physical methods for seed invigoration: advantages and challenges in seed technology, Front. Plant Sci., 7 (2016), 646.
    [8] F. Anjum, F. Anwar, A. Jamil, M. Iqbal, Microwave roasting effects on the physico‐chemical composition and oxidative stability of sunflower seed oil, J. Am. Oil Chem. Soc., 83 (2006), 777–784. doi: 10.1007/s11746-006-5014-1
    [9] B. K. Aarthi, V. Aswini, M. L. Priya, M. Nirosha, M. Shanmugaprakash, Optimization of Microwave Assisted Extraction of Pectin from Helianthus annuus Head Using Response Surface Methodology, in Biotechnology and Biochemical Engineering (eds. B. D. Prasanna, G. Sathyanarayana, V. Praveen), Springer, 2016, 35–45.
    [10] M. Porcelli, G. Cacciapuoti, S. Fusco, R. Massa, G. d'Ambrosio, C. Bertoldo, et al., Non‐thermal effects of microwaves on proteins: thermophilic enzymes as model system, FEBS Lett., 402 (1997), 102–106. doi: 10.1016/S0014-5793(96)01505-0
    [11] K. Mohammed, M. Koko, M. Obadi, K. S. Letsididi, P. Cao, Y. Liu, Effects of Microwave Roasting Process and Time on Chemical Composition and Oxidative Stability of Sunflower Oil, J. Food Nutr. Res., 5 (2017), 659–667.
    [12] J. Mrda, J. Crnobarac, N. Dusanic, S. Jocić V. Miclic, Germination energy as a parameter of seed quality in different sunflower genotypes, Genetica, 43 (2011), 427-436.
    [13] B. J. Davis, Disc electrophoresis–II method and application to human serum proteins, Ann. N. Y. Acad. Sci., 121 (1964), 404–427.
    [14] L. Ornstein, Disc electrophoresis I. background and theory, Ann. N. Y. Acad. Sci., 121 (1964), 321–349.
    [15] R. Westermeier, Electrophoresis in Practice, 3rd Edition, WILEY-VCH, Weinheim, 2001.
    [16] P. K. Robinson, Enzymes: principles and biotechnological applications, Essays Biochem., 59 (2015), 1–41. doi: 10.1042/bse0590001
    [17] G. P. Manchenko, Handbook of Detection of Enzymes on Electrophoretic Gels, CRC Press, 2002.
    [18] A. V. Loskutov, I. G. Borovkova, Identifying sunflower lines with the help of isoenzymes, Bull. All Russ. Res. Inst. Oil Crops, 4 (1990), 17–18.
    [19] H. M. Pallavi, K. Ramegowda, K. Vishwanath, Y. K. Shadakshari, Evaluation and confirmation of hybridity on the basis of seed proteins, Res. J. Agr. Sci., 1 (2010), 189–192.
    [20] B. Duissenbekov, A. Tokmuratov, N. Zhangabay, Z. Orazbayev, B. Yerimbetov, Z. Aldiyarov, Finite-difference equations of quasistatic motion of the shallow concrete shells in nonlinear setting, Curved Layer. Struct., 7 (2020), 48–55. doi: 10.1515/cls-2020-0005
    [21] V. I. Tishkov, V. O. Popov, The way formate dehydrogenase acts and its practical application (in Russia), Biochemistry, 69 (2004), 1537–1554.
    [22] J. Zhao, T. D. Missihoun, D. Bartels, The role of Arabidopsis aldehyde dehydrogenase genes in response to high temperature and stress combinations, J. Exp. Bot., 68 (2017), 4295–4308. doi: 10.1093/jxb/erx194
    [23] I. H. Segel, Effects of pH and temperature, in Enzyme kinetics: behavior and analysis of rapid equilibrium and steady-state enzyme systems (ed. I. H. Segel), Wiley-Intersciences, New York, 1975,926–941.
    [24] J. X. Cao, F. Wang, X. Li, Y. Y. Sun, Y. Wang, C. R. Ou, et al., The influence of microwave sterilization on the ultrastructure, permeability of cell membrane and expression of proteins of Bacillus cereus, Front. Microbiol., 9 (2018), 1870. doi: 10.3389/fmicb.2018.01870
  • Reader Comments
  • © 2021 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Metrics

Article views(1025) PDF downloads(101) Cited by(0)

Article outline

Figures and Tables

Figures(3)  /  Tables(2)

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return

Catalog