AIMS Microbiology, 2017, 3(4): 762-773. doi: 10.3934/microbiol.2017.4.762.

Research article

Export file:

Format

  • RIS(for EndNote,Reference Manager,ProCite)
  • BibTex
  • Text

Content

  • Citation Only
  • Citation and Abstract

Effect of soil storage at 4 °C on the calorespirometric measurements of soil microbial metabolism

1 Department of Applied Physics, University of Santiago de Compostela, Santiago de Compostela 15782, Spain
2 Department of Agroforestry Engineering, University of Santiago de Compostela, Lugo 27002, Spain
3 Department of Chemistry and Biochemistry, Brigham Young University, Provo, UT 84602, USA

Soil samples must usually be stored for a time between collection and measurements of microbial metabolic properties. However, little is known about the influence of storage conditions on microbial metabolism when studied by calorespirometry. Calorespirometry measures the heat rate and the CO2 rate of microbial metabolism, where the ratio of heat and CO2 released, the calorespirometric ratio, informs about the nature of substrates being used by microorganisms. Application to soil microbiology is very recent, and little is known about the influence of the common soil preparation practices between collection and analysis on the calorespirometric measurements. For these reasons, the effect of storage at 4 °C on the microbial metabolism was determined by calorespirometry. Results show CO2 production rate decreases with storage time while the evolution of metabolic heat rate is more stable. The calorespirometric ratio increases with storage time in soil samples with organic matter characterized by lower carbohydrate contribution to the total carbon and higher aromaticity and is unaffected in soil samples with lower carbohydrates in the organic matter and higher aromaticity. Therefore, the calorespirometric ratio values may vary for the same soil sample, such that the soil organic matter properties, as well as the time stored at 4 °C, must be considered in interpreting calorespirometric data on soils.
  Figure/Table
  Supplementary
  Article Metrics

Keywords soil; storage; heat rate; CO2 rate; calorespirometric ratio; microbial metabolism

Citation: Nieves Barros, Sergio Feijoo, César Pérez-Cruzado, Lee D. Hansen. Effect of soil storage at 4 °C on the calorespirometric measurements of soil microbial metabolism. AIMS Microbiology, 2017, 3(4): 762-773. doi: 10.3934/microbiol.2017.4.762

References

  • 1. Šimek M, Santruckova H (1999) Influence of storage of soil samples on microbial biomass and its activity. Rost Vyroba 44: 415–419.
  • 2. Yanai Y, Toyota K, Okazaki M (2007) Response of denitrifying communities to successive soil freeze-thaw cycles. Biol Fert Soils 44: 113–119.    
  • 3. Lauber CL, Zhou N, Gordon JI, et al. (2010) Effect of storage conditions on the assessment of bacterial community structure in soil and human-associated samples. FEMS Microbiol Lett 307: 80–86.    
  • 4. Lee YB, Lorenz N, Dick LK, et al. (2006) Cold storage and pre-treatment incubation effects on soil microbial properties. Soil Sci Soc Am J 71: 1299–1305.
  • 5. Rubin BER, Gibbons SM, Kennedy S, et al. (2013) Investigating the impact of storage conditions on microbial community composition in soil samples. PLoS One 8: 1–9.
  • 6. Stenberg B, Johansson M, Pell M, et al. (1998) Microbial biomass and activities in soils as affected by frozen and cold storage. Soil Biol Biochem 30: 393–402.    
  • 7. Larsen KS, Jonasson S, Michelsen A (2002) Repeated freeze-thaw cycles and their effects on biological processes in two arctic ecosystem types. Appl Soil Ecol 21: 187–195.    
  • 8. Barros N, Salgado J, Feijoo S (2007) Calorimetry and soil. Thermochim Acta 458: 11–17.    
  • 9. Rong XM, Huang QY, Jiang DH, et al. (2007) Isothermal microcalorimetry: A review of application in soil and environmental sciences. Pedosphere 17: 137–145.    
  • 10. Xu J, Feng Y, Barros N, et al. (2017) Exploring the potential of microcalorimetry to study soil microbial metabolic diversity. J Therm Anal Calorim 127: 1457–1465.    
  • 11. Barros N, Hansen LD, Piñeiro V, et al. (2016) Calorimetry measures the response of soil organic matter biodegradation to increasing temperature. J Therm Anal Calorim 123: 2397–2403.    
  • 12. Barros N, Feijoo S, Balsa R, et al. (2017) Calorimetry reveals the response of soil microbial metabolism to increasing temperature in soils with different thermal, chemical and biological properties. Adv Mat Tech Env 1: 27–37.
  • 13. Barros N, Feijoo S (2003) A combined mass and energy balance to provide bioindicators of soil microbiological quality. Biophys Chem 104: 561–572.    
  • 14. Harris JA, Ritz K, Coucheney E, et al. (2012) The thermodynamic efficiency of soil microbial communities subject to long-term stress is lower than those under conventional input regimes. Soil Biol Biochem 47: 149–157.    
  • 15. Wadsö L, Hansen LD (2015) Calorespirometry of terrestrial organisms and ecosystems. Methods 76: 11–19.    
  • 16. Barros N, Feijoo S, Hansen L (2011) Calorimetric determination of metabolic heat, CO2 rates and the calorespirometric ratio of soil basal metabolism. Geoderma 160: 542–547.    
  • 17. Herrmann AM, Bölscher T (2015) Simultaneous screening of microbial energetics and CO2 respiration in soil samples from different ecosystems. Soil Biol Biochem 83: 88–92.    
  • 18. Brueckner D, Solokhina A, Krähenbühl S, et al. (2017) A combined application of tunable diode laser absorption spectroscopy and isotherm micro-calorimetry for calorespirometric analysis. J Microbiol Meth 139: 210.    
  • 19. Barros N, Hansen LD, Piñeiro V, et al. (2016) Factors influencing the calorespirometric ratios of soil microbial metabolism. Soil Biol Biochem 92: 221–229.    
  • 20. Barros N, Piñeiro V, Hansen LD (2015) Calorespirometry: A novel tool to assess the effect of temperature on soil organic matter decomposition. Thermochim Acta 618: 15–17.    
  • 21. Hansen LD, Macfarlane C, McKinnon N, et al. (2004) Use of calorespirometric ratios, heat per CO2 and heat per O2, to quantify metabolic paths and energetics of growing cells.Thermochim Acta 422: 55–61.    
  • 22. Barros N, Merino A, Martín-Pastor M, et al. (2014) Changes in soil organic matter in a forestry chronosequence monitored by thermal analysis and calorimetry. SJSS 4: 239–253.
  • 23. Núñez-Regueira L, Barros N, Barja I (1994) Effect of storage of soil at 4 °C on the microbial activity studied by microcalorimetry. J Therm Anal Calorim 41: 1379–1383.    
  • 24. Pérez-Cruzado C, Sande B, Omil B, et al. (2014) Organic matter properties in soils afforested with Pinus radiata. Plant Soil 374: 381–398.    
  • 25. Plante AF, Fernández JM, Haddix ML, et al. (2011) Biological, chemical and thermal indices of soil organic matter stability in four grasslands soils. Soil Biol Biochem 43: 1051–1058.    
  • 26. Pesaro M, Nicollier G, Zeyer J, et al. (2004) Impact of soil drying-rewetting stress on microbial communities and activities and on degradation of two crop protection products. Appl Environ Microb 70: 2577–2587.    
  • 27. R Core Team, R: A language and environment for statistical computing, R Foundation for Statistical Computing, Vienna, Austria, 2015. Available from: https://www.R-project.org/.
  • 28. Lebuhn M, Heilmann B, Hartmann A (1994) Effects of drying/rewetting stress on microbial auxin production and L-tryptophan catabolism. Biol Fert Soils 18: 302–310.    
  • 29. Coxson ES, Parkinson D (1987) Winter respiratory activity in aspen woodland forest floor litter and soils. Soil Biol Biochem 19: 49–59.    
  • 30. Nunan L, Lerch TZ, Pouteau V, et al. (2015) Metabolising old soil carbon: simply a matter of simply organic matter? Soil Biol Biochem 88: 128–136.    

 

Reader Comments

your name: *   your email: *  

Copyright Info: © 2017, Nieves Barros, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

Download full text in PDF

Export Citation

Copyright © AIMS Press All Rights Reserved