AIMS Microbiology, 2016, 2(2): 152-189. doi: 10.3934/microbiol.2016.2.152.

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Comparison of transcriptomes of enlarged spheroplasts of Erythrobacter litoralis and Lelliottia amnigena

1 Biotechnology Research Center and Department of Biotechnology, Toyama Prefectural University, Imizu, Toyama 939-0398, Japan
2 Graduate School of Biological Sciences, Nara Institute of Science and Technology, Takayama, Ikoma, Nara 630-0192, Japan

Bacterial spheroplasts do not divide but they grow and enlarge with DNA replication in a broth containing an inhibitor of peptidoglycan synthesis and high salt concentration. The enlarged spheroplasts of Lelliottia amnigena, belonging to the family Enterobacteriaceae, formed vacuole-like structures, while those of the aerobic photosynthetic marine bacterium Erythrobacter litoralis did not form such structures. In addition, the enlarged spheroplasts of L. amnigena, which differ in the size of inner and outer membranes, were larger than those of E. litoralis. To elucidate the reason for these differences, we analyzed the transcriptome (RNA-seq) of spheroplasts at the beginning of growth and upon enlargement. After sequencing the RNAs expressed in normal and enlarged spheroplasts, we compared the gene expression levels of the 1100 orthologs in E. litoralis and L. amnigena. Among these, 347 and 213 genes were more than 2-fold upregulated in enlarged spheroplasts of E. litoralis and L. amnigena, respectively; 193 and 269 genes were less than 0.5-fold downregulated in those of E. litoralis and L. amnigena, respectively. Thirty six genes were upregulated in L. amnigena enlarged spheroplasts but were downregulated in E. litoralis enlarged spheroplasts, and may be related to vacuole-like structure generation. Fourteen of the 36 genes encoded a membrane protein. Our findings indicate that spheroplast enlargement varies between different organisms with respect to gene expression.
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Keywords enlarged spheroplast; Erythrobacter litoralis; inner membrane; Lelliottia amnigena; outer membrane; transcriptome; vacuole-like structure

Citation: Sawako Takahashi, Ayana Takayanagi, Yurika Takahashi, Taku Oshima, Hiromi Nishida. Comparison of transcriptomes of enlarged spheroplasts of Erythrobacter litoralis and Lelliottia amnigena. AIMS Microbiology, 2016, 2(2): 152-189. doi: 10.3934/microbiol.2016.2.152

References

  • 1. Lederberg J (1956) Bacterial protoplasts induced by penicillin. Proc Natl Acad Sci USA 42: 574–577.    
  • 2. Lederberg J, St Clair J (1958) Protoplasts and L-type growth of Escherichia coli. J Bacteriol 75: 143–160.
  • 3. Kusaka I (1967) Growth and division of protoplasts of Bacillus megaterium and inhibition of division by penicillin. J Bacteriol 94: 884–888.
  • 4. Kuroda T, Okuda N, Saitoh N, et al. (1998) Patch clamp studies on ion pumps of the cytoplasmic membrane of Escherichia coli. J Biol Chem 273: 16897–16904.    
  • 5. Nakamura K, Ikeda S, Matsuo T, et al. (2011) Patch clamp analysis of the respiratory chain in Bacillus subtilis. Biochim Biophys Acta 1808: 1103–1107.    
  • 6. Takahashi S, Nishida H (2015) Quantitative analysis of chromosomal and plasmid DNA during the growth of spheroplasts of Escherichia coli. J Gen Appl Microbiol 61: 262–265.    
  • 7. Takayanagi A, Takahashi S, Nishida H (2016) Requirement of dark culture condition for enlargement of spheroplasts of the aerobic anoxygenic photosynthetic marine bacterium Erythrobacter litoralis. J Gen Appl Microbiol 62: 14–17.    
  • 8. Paradis S, Boissinot M, Paquette N, et al. (2005) Phylogeny of the Enterobacteriaceae based on genes encoding elongation factor Tu and F-ATPase β-subunit. Int J Syst Evol Microbiol 55: 2013–2025.
  • 9. Takahashi S, Nishida H (2016) Growth of Enterobacter amnigenus and Escherichia coli spheroplasts in marine broth containing penicillin. Bull Toyama Pref Univ 26: 27–30.
  • 10. Yurkov VV, Beatty JT (1998) Aerobic anoxygenic phototrophic bacteria. Microbiol Mol Biol Rev 62: 695–724.
  • 11. Fisher RA (1922) On the interpretation of χ2 from contingency tables, and the calculation of P. J Royal Statist Soc 85: 87–94.    
  • 12. Narita S, Tokuda H (2009) Biochemical characterization of an ABC transporter LptBFGC complex required for the outer membrane sorting of lipopolysaccharides. FEBS Lett 583: 2160–2164.    
  • 13. Watanabe S, Ohbayashi R, Kanasaki Y, et al. (2015) Intensive DNA replication and metabolism during the lag phase in cyanobacteria. PLOS ONE 10: e0136800.    
  • 14. Bensaid A, Almeida A, Drlica K, et al. (1996) Cross-talk between topoisomerase I and HU in Escherichia coli. J Mol Biol 256: 292–300.    
  • 15. Beam CE, Saveson CJ, Lovett ST (2002) Role for radAsms in recombination intermediate processing in Escherichia coli. J Bacteriol 184: 6836–6844.    
  • 16. Ranjit DK, Young KD (2013) The Rcs stress response and accessory envelope proteins are required for de novo generation of cell shape in Escherichia coli. J Bacteriol 195: 2452–2462.    
  • 17. Gibson MM, Bagga DA, Miller CG, et al. (1991) Magnesium transport in Salmonella typhimurium: the influence of new mutations conferring Co2+ resistance on the CorA Mg2+ transport system. Mol Microbiol 5: 2753–2762.    

 

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Copyright Info: 2016, Hiromi Nishida, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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