Research article Special Issues

The regulation of miR-139-5p on the biological characteristics of breast cancer cells by targeting COL11A1

  • Received: 30 May 2019 Accepted: 12 November 2019 Published: 27 November 2019
  • ObjectiveThis study aimed to explore the regulatory mechanism of miR-139-5p on the biological characteristics of breast cancer cells by targeting Collagen type XI alpha 1 chain (COL11A1). MethodGEO2R was used to identify the differentially expressed genes (DEGs) of breast cancer in GEO database. miRDB, miRanda and TargetScan databases were used to predict the miRNAs that regulate COL11A1. qRT-PCR was used to detect the expressions of COL11A1 and miR-139-5p in breast cancer cells, and western blot was used to detect the protein level of COL11A1. RNA binding protein immunoprecipitation assay was employed to test the targeted relationship between miR-139-5p and COL11A1, which was further verified by dual-luciferase reporter gene assay. CCK-8 assay was performed to detect the cell proliferation, and flow cytometry was carried out to examine the cell apoptosis. Moreover, western blot was used for the detection of caspase-3, Bax and Bcl-2 protein levels. ResultsA total of five DEGs were screened from the GEO database, of which COL11A1 was the only highly expressed in breast cancer. According to the database analysis, we predicted that miR-139-5p was much likely to target the expression of COL11A1. MiR-139-5p was poorly expressed in breast cancer cells and targeted inhibited COL11A1. Silencing COL11A1 or overexpressing miR-139-5p both could inhibit the proliferation and promote the apoptosis, while overexpressing the two factors simultaneously could reverse such effect. ConclusionOverexpression of miR-139-5p inhibits the proliferation and promotes the apoptosis of breast cancer cells by inhibiting the expression of COL11A1.

    Citation: Shequn Gu, Jihui Luo, Wenxiu Yao. The regulation of miR-139-5p on the biological characteristics of breast cancer cells by targeting COL11A1[J]. Mathematical Biosciences and Engineering, 2020, 17(2): 1428-1441. doi: 10.3934/mbe.2020073

    Related Papers:

  • ObjectiveThis study aimed to explore the regulatory mechanism of miR-139-5p on the biological characteristics of breast cancer cells by targeting Collagen type XI alpha 1 chain (COL11A1). MethodGEO2R was used to identify the differentially expressed genes (DEGs) of breast cancer in GEO database. miRDB, miRanda and TargetScan databases were used to predict the miRNAs that regulate COL11A1. qRT-PCR was used to detect the expressions of COL11A1 and miR-139-5p in breast cancer cells, and western blot was used to detect the protein level of COL11A1. RNA binding protein immunoprecipitation assay was employed to test the targeted relationship between miR-139-5p and COL11A1, which was further verified by dual-luciferase reporter gene assay. CCK-8 assay was performed to detect the cell proliferation, and flow cytometry was carried out to examine the cell apoptosis. Moreover, western blot was used for the detection of caspase-3, Bax and Bcl-2 protein levels. ResultsA total of five DEGs were screened from the GEO database, of which COL11A1 was the only highly expressed in breast cancer. According to the database analysis, we predicted that miR-139-5p was much likely to target the expression of COL11A1. MiR-139-5p was poorly expressed in breast cancer cells and targeted inhibited COL11A1. Silencing COL11A1 or overexpressing miR-139-5p both could inhibit the proliferation and promote the apoptosis, while overexpressing the two factors simultaneously could reverse such effect. ConclusionOverexpression of miR-139-5p inhibits the proliferation and promotes the apoptosis of breast cancer cells by inhibiting the expression of COL11A1.


    加载中


    [1] F. Bray, J. Ferlay, I. Soerjomataram, R. L. Siegel, L. A. Torre, A. Jemal, Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries, CA. Cancer J. Clin., 68 (2018), 394-424.
    [2] H. Fang, J. Xie, M. Zhang, Z. Zhao, Y. Wan, Y. Yao, miRNA-21 promotes proliferation and invasion of triple-negative breast cancer cells through targeting PTEN, Am. J. Transl. Res., 9 (2017), 953-961.
    [3] Y. Xie, P. Zong, W. Wang, D. Liu, B. Li, Y. Wang, Hypermethylation of potential tumor suppressor miR-34b/c is correlated with late clinical stage in patients with soft tissue sarcomas, Exp. Mol. Pathol., 98 (2015), 446-454.
    [4] D. Wu, P. Lu, X. Mi, J. Miao, Downregulation of miR-503 contributes to the development of drug resistance in ovarian cancer by targeting PI3K p85, Arch. Gynecol. Obstet., 297 (2018), 699-707.
    [5] X. Zhang, D. Ren, X. Wu, X. Lin, L. Ye, C. Lin, miR-1266 Contributes to Pancreatic Cancer Progression and Chemoresistance by the STAT3 and NF-kappaB Signaling Pathways, Mol. Ther. Nucleic. Acids., 11 (2018), 142-158.
    [6] Y. You, X. Wang, M. Xu, J. Zhao, Expression and clinical significance of miR-139-5p in non-small cell lung cancer, J. Int. Med. Res., 47 (2019), 867-874.
    [7] J. Tu, Z. Zhao, M. Xu, X. Lu, L. Chang, J. Ji, NEAT1 upregulates TGF-beta1 to induce hepatocellular carcinoma progression by sponging hsa-mir-139-5p, J. Cell. Physiol., 233 (2018), 8578-8587.
    [8] B. Yang, W. Zhang, D. Sun, X. Wei, Y. Ding, Y. Ma, Downregulation of miR-139-5p promotes prostate cancer progression through regulation of SOX5, Biomed. Pharmacother., 109 (2019), 2128-2135.
    [9] H. Fischer, R. Stenling, C. Rubio, A. Lindblom, Colorectal carcinogenesis is associated with stromal expression of COL11A1 and COL5A2, Carcinogenesis, 22 (2001), 875-878.
    [10] Y. Shintani, M. A. Hollingsworth, M. J. Wheelock, K. R. Johnson, Collagen I promotes metastasis in pancreatic cancer by activating c-Jun NH(2)-terminal kinase 1 and up-regulating N-cadherin expression, Cancer Res., 66 (2006), 11745-11753.
    [11] A. Menke, C. Philippi, R. Vogelmann, B. Seidel, M. P. Lutz, G. Adler, Down-regulation of E-cadherin gene expression by collagen type I and type III in pancreatic cancer cell lines, Cancer Res., 61 (2001), 3508-3517.
    [12] H. Yoshioka, P. Greenwel, K. Inoguchi, S. Truter, Y. Inagaki, Y. Ninomiya, Structural and functional analysis of the promoter of the human alpha 1(XI) collagen gene, J. Biol. Chem., 270 (1995), 418-424.
    [13] D. Sun, H. Jin, J. Zhang, X. Tan, Integrated whole genome microarray analysis and immunohistochemical assay identifies COL11A1, GJB2 and CTRL as predictive biomarkers for pancreatic cancer, Cancer Cell Int., 18 (2018), 174.
    [14] V. Maire, F. Nemati, M. Richardson, A. V. Salomon, B. Tesson, G. Rigaill, Polo-like kinase 1: A potential therapeutic option in combination with conventional chemotherapy for the management of patients with triple-negative breast cancer, Cancer Res., 73 (2013), 813-823.
    [15] C. Clarke, S. F. Madden, P. Doolan, S. T. Aherne, H. Joyce, L. O'Driscoll, Correlating transcriptional networks to breast cancer survival: a large-scale coexpression analysis, Carcinogenesis, 34 (2013), 2300-2308.
    [16] M. Xie, T. Ma, J. Xue, H. Ma, M. Sun, Z. Zhang, The long intergenic non-protein coding RNA 707 promotes proliferation and metastasis of gastric cancer by interacting with mRNA stabilizing protein HuR, Cancer Lett., 443 (2019), 67-79.
    [17] D. Diaz, A. Prieto, E. Reyes, H. BarcenillaJorge, M. Melchor, A. Mon, Flow cytometry enumeration of apoptotic cancer cells by apoptotic rate, Methods Mol. Biol., 1219 (2015), 11-20.
    [18] Z. L. Sun, S. C. Zhang, H. L. Li, Y. C. Gao, EBP50 inhibits the invasion and metastasis of breast cancer cells through Wnt3a/β-catenin signaling pathway, Chin. J. Biochem. Mol. Bio. (2016), 1027-1032.
    [19] J. Li, J. Zhang, Y. Wang, X. Liang, Z. Wusiman, Y. Yin, Synergistic inhibition of migration and invasion of breast cancer cells by dual docetaxel/quercetin-loaded nanoparticles via Akt/MMP-9 pathway, Int. J. Pharm., 523 (2017), 300-309.
    [20] L. Badea, V. Herlea, S. O. Dima, T. Dumitrascu, I. Popescu, Combined gene expression analysis of whole-tissue and microdissected pancreatic ductal adenocarcinoma identifies genes specifically overexpressed in tumor epithelia, Hepatogastroenterology, 55 (2008), 2016-2027.
    [21] I. W. Chong, M. Y. Chang, H. C. Chang, Y. P. Yu, C. C. Sheu, J. R. Tsai, Great potential of a panel of multiple hMTH1, SPD, ITGA11 and COL11A1 markers for diagnosis of patients with non-small cell lung cancer, Oncol. Rep., 16 (2006), 981-988.
    [22] Y. H. Wu, Y. F. Huang, C. C. Chen, C. Y. Chou, Akt inhibitor SC66 promotes cell sensitivity to cisplatin in chemoresistant ovarian cancer cells through inhibition of COL11A1 expression, Cell. Death. Dis., 10 (2019), 322.
    [23] T. Rizou, F. Perlikos, M. Lagiou, M. Karaglani, S. Nikolopoulos, I. Toumpoulis, Development of novel real-time RT-qPCR methodologies for quantification of the COL11A1 mRNA general and C transcripts and evaluation in non-small cell lung cancer specimens, J. BU ON., 23 (2018), 1699-1710.
    [24] A. Li, J. Li, J. Lin, W. Zhuo, J. Si, COL11A1 is overexpressed in gastric cancer tissues and regulates proliferation, migration and invasion of HGC-27 gastric cancer cells in vitro, Oncol. Rep., 37 (2017), 333-340. doi: 10.3892/or.2016.5276
    [25] Y. H. Wu, Y. F. Huang, T. H. Chang, C. Y. Chou, Activation of TWIST1 by COL11A1 promotes chemoresistance and inhibits apoptosis in ovarian cancer cells by modulating NF-kappaB-mediated IKKbeta expression, Int. J. Cancer, 141 (2017), 2305-2317.
    [26] K. Tu, X. Zheng, C. Dou, C. Li, W. Yang, Y. Yao, MicroRNA-130b promotes cell aggressiveness by inhibiting peroxisome proliferator-activated receptor gamma in human hepatocellular carcinoma, Int. J. Mol. Sci., 15 (2014), 20486-20499.
    [27] X. Zhu, J. Qiu, T. Zhang, Y. Yang, S. Guo, T. Li, MicroRNA-188-5p promotes apoptosis and inhibits cell proliferation of breast cancer cells via the MAPK signaling pathway by targeting Rap2c, J. Cell Physiol. 2019 (2019).
    [28] M. N. Menbari, K. Rahimi, A. Ahmadi, S. M. Yeganeh, A. Elyasi, N. Darvishi, MiR-483-3p suppresses the proliferation and progression of human triple negative breast cancer cells by targeting the HDAC8 oncogene, J. Cell Physiol. 2019 (2019).
    [29] Z. Ghaemi, B. M. Soltani, S. J. Mowla, MicroRNA-326 Functions as a Tumor Suppressor in Breast Cancer by Targeting ErbB/PI3K Signaling Pathway, Front. Oncol., 9 (2019), 653.
    [30] Q. Li, X. Liang, Y. Wang, X. Meng, Y. Xu, S. Cai, MiR-139-5p Inhibits the Epithelial-Mesenchymal Transition and Enhances the Chemotherapeutic Sensitivity of Colorectal Cancer Cells by Downregulating BCL2, Sci. Rep., 6 (2016), 27157.
    [31] J. Liu, C. Li, Y. Jiang, Y. Wan, S. Zhou, Wen. Cheng, Tumor-suppressor role of miR-139-5p in endometrial cancer, Cancer Cell Int., 18 (2018), 51.
    [32] M. Yonemori, N. Seki, H. Yoshino, R. Matsushita, K. Miyamoto, M. Nakagawa, Dual tumor-suppressors miR-139-5p and miR-139-3p targeting matrix metalloprotease 11 in bladder cancer, Cancer Sci., 107 (2016), 1233-1242.
    [33] H. D. Zhang, D. W. Sun, L. Mao, J. Zhang, L. Jiang, MiR-139-5p inhibits the biological function of breast cancer cells by targeting Notch1 and mediates chemosensitivity to docetaxel, Biochem. Biophys. Res. Commun., 465 (2015), 702-713.
    [34] K. Krishnan, A. L. Steptoe, H. C. Martin, D. R. Pattabiraman, K. Nones, N. Waddell, MiR-139-5p is a regulator of metastatic pathways in breast cancer, RNA, 19 (2013), 1767-1780.
  • Reader Comments
  • © 2020 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
通讯作者: 陈斌, bchen63@163.com
  • 1. 

    沈阳化工大学材料科学与工程学院 沈阳 110142

  1. 本站搜索
  2. 百度学术搜索
  3. 万方数据库搜索
  4. CNKI搜索

Metrics

Article views(3991) PDF downloads(474) Cited by(18)

Article outline

Figures and Tables

Figures(5)  /  Tables(1)

Other Articles By Authors

/

DownLoad:  Full-Size Img  PowerPoint
Return
Return

Catalog