Research article

Molecular characterization of the netrin-1 UNC-5 receptor in Lucilia sericata larvae

  • Received: 19 June 2019 Accepted: 02 August 2019 Published: 08 August 2019
  • Larval therapy with Lucilia sericata is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. Netrin-1, one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The UNC-5 receptor combines with a netrin-1 signal and transmits the signal from one side of the membrane to the other side, initiating a change in cell activity. In the current study, we identified the full length of the UNC-5 receptor mRNA in L. sericata using different sets of primers, including exon junction and specific region primers. The coding sequence (CDS) of the UNC-5 receptor was sequenced and identified to include 633 base-pair nucleic acids, and BLAST analysis on its nucleotide sequence revealed 96% identity with the Lucilia cuprina netrin-1 UNC-5 receptor. The protein residue included 210 amino acids (aa) and coded for a protein with 24 kD weight. This gene lacked the signal peptide. Furthermore, the UPA domain is conserved in UNC-5. It lied at the interval of 26–131 aa. We identified the CDS of netrin-1 UNC-5 receptor in L. sericata. It could be applied to research activities implementing a new essential component design in wound healing.

    Citation: Tahereh Karamzadeh, Hamzeh Alipour, Marziae Shahriari-Namadi, Abbasali Raz, Kourosh Azizi, Masoumeh Bagheri, Mohammad D. Moemenbellah-Fard. Molecular characterization of the netrin-1 UNC-5 receptor in Lucilia sericata larvae[J]. AIMS Genetics, 2019, 6(3): 46-54. doi: 10.3934/genet.2019.3.46

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  • Larval therapy with Lucilia sericata is a promising strategy in wound healing. Axon guidance molecules play vital roles during the development of the nervous system and also regulate the capacity of neuronal restoration in wound healing. Netrin-1, one of the proteins that larvae secrete, plays a useful role in cell migration and nerve tissue regeneration. The UNC-5 receptor combines with a netrin-1 signal and transmits the signal from one side of the membrane to the other side, initiating a change in cell activity. In the current study, we identified the full length of the UNC-5 receptor mRNA in L. sericata using different sets of primers, including exon junction and specific region primers. The coding sequence (CDS) of the UNC-5 receptor was sequenced and identified to include 633 base-pair nucleic acids, and BLAST analysis on its nucleotide sequence revealed 96% identity with the Lucilia cuprina netrin-1 UNC-5 receptor. The protein residue included 210 amino acids (aa) and coded for a protein with 24 kD weight. This gene lacked the signal peptide. Furthermore, the UPA domain is conserved in UNC-5. It lied at the interval of 26–131 aa. We identified the CDS of netrin-1 UNC-5 receptor in L. sericata. It could be applied to research activities implementing a new essential component design in wound healing.


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    Acknowledgment



    We appreciate colleagues in the Department of Medical Entomology, School of Health, for their collaboration in this research activity. This project was funded by the Shiraz University of Medical Sciences (SUMS), Shiraz, Iran [Grant #: 1396-01-04-15875] allocated to H.A. It was part of an MSc thesis in Medical Entomology and Vector Control solicited to the student, Ms. T. Karamzadeh.

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