Research article

Development of sample preparation method for organochlorine pesticides analysis in soil samples

  • Received: 23 August 2020 Accepted: 29 September 2020 Published: 30 September 2020
  • OCPs are the persistent organic pollutants which are highly toxic to the environment and can accumulate in humans by foods such as rice, vegetable, and fruits. The aim of this research was to develop a rapid and easy sample preparation method for determination 4 OCPs, aldrin, dieldrin, β-endosulfane, and p-p' DDT in soil samples by gas chromatography with electron capture detection. The analysis was performed by using HP-5 capillary column with the isothermal condition at 250 oC and carrier gas flow rate of 1 mL/min. The results show that the 4 OCPs and internal standard, pentachloronitrobenzene, were analyzed within 6 min. The optimized condition of the sample preparation method was using ultrasonic assisted solvent extraction with 20 mL of hexane and ethyl acetate (9:1 v/v) as an extractant for 15x2 min. The results obtained from the proposed sample preparation method have demonstrated that the limit of detection was 0.628–3.68 μg kg-1. Percent recoveries were in the range of 81.42 to 110.7% and RSD were 1.68–9.43%. Correlation coefficients of calibration graphs were more than 0.99. The developed sample preparation method exhibited simplicity rapid and low chemical reagent consumption with satisfactory results.

    Citation: Thanawat Jumepaeng, Nirand Pongpun. Development of sample preparation method for organochlorine pesticides analysis in soil samples[J]. AIMS Environmental Science, 2020, 7(5): 380-386. doi: 10.3934/environsci.2020025

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  • OCPs are the persistent organic pollutants which are highly toxic to the environment and can accumulate in humans by foods such as rice, vegetable, and fruits. The aim of this research was to develop a rapid and easy sample preparation method for determination 4 OCPs, aldrin, dieldrin, β-endosulfane, and p-p' DDT in soil samples by gas chromatography with electron capture detection. The analysis was performed by using HP-5 capillary column with the isothermal condition at 250 oC and carrier gas flow rate of 1 mL/min. The results show that the 4 OCPs and internal standard, pentachloronitrobenzene, were analyzed within 6 min. The optimized condition of the sample preparation method was using ultrasonic assisted solvent extraction with 20 mL of hexane and ethyl acetate (9:1 v/v) as an extractant for 15x2 min. The results obtained from the proposed sample preparation method have demonstrated that the limit of detection was 0.628–3.68 μg kg-1. Percent recoveries were in the range of 81.42 to 110.7% and RSD were 1.68–9.43%. Correlation coefficients of calibration graphs were more than 0.99. The developed sample preparation method exhibited simplicity rapid and low chemical reagent consumption with satisfactory results.


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  • © 2020 the Author(s), licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0)
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