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Crude oil degradation potential of bacteria isolated from oil-polluted soil and animal wastes in soil amended with animal wastes

1 Department of Environmental Management and Toxicology, Federal University of Petroleum Resources, Effurun, Nigeria
2 Department of Microbiology, Delta State University, Abraka, Nigeria

The influence of animal wastes on crude oil degradation potential of strains of Proteus vulgaris and Bacillus subtilis isolated from animal wastes (poultry and pig droppings) and petroleum-polluted soil was compared in laboratory studies. Both bacterial strains were selected for high crude oil degradation ability after screening many isolates by the 2,6-dichlorophenol indophenol method. Analyses by gas chromatography (GC) showed that degradation of crude oil was markedly enhanced (88.3–97.3% vs 72.1–78.8%) in soil amended with animal wastes as indicated by the reduction of total petroleum hydrocarbon (TPH). TPH reduction by animal waste bacterial strains in animal waste-amended soil was more than the reduction by strains from soil contaminated with petroleum (P < 0.001). The greatest reduction of TPH (96.6–97.3% vs 80.4–95.9%) was by poultry waste strains and it occurred in soil amended with poultry waste. GC analyses of n-alkanes showed that although shorter chains were preferentially degraded [32.0–78.5% (C8–23) vs 6.3–18.5% (C24–36)] in normal soil, biodegradation of longer chains increased to 38.4–46.3% in animal waste-amended soil inoculated with the same animal wastes’ strains. The results indicate that these animal waste strains may be of potential application for bioremediation of oil-polluted soil in the presence of the wastes from where they were isolated.
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Keywords poultry wastes; pig wastes; crude oil; biodegradation; Proteus vulgaris; Bacillus subtilis

Citation: Voke O. Urhibo, Bernard O. Ejechi. Crude oil degradation potential of bacteria isolated from oil-polluted soil and animal wastes in soil amended with animal wastes. AIMS Environmental Science, 2017, 4(2): 277-286. doi: 10.3934/environsci.2017.2.277

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