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Lignocellulose bioconversion to ethanol by a fungal single-step consolidated method tested with waste substrates and co-culture experiments

  • Received: 30 June 2018 Accepted: 10 October 2018 Published: 18 October 2018
  • The Polyporales phlebioid white rot fungus Phlebia radiata is efficient in decomposing the wood main components, and in producing ethanol from lignocelluloses and waste materials. Based to these qualifications, the fungus was adopted for design of a consolidated bioprocess method to convert wood waste materials into ethanol without pretreatments. Higher ethanol yield was aimed by introducing collaborative fungal cultivations including isolates of Saccharomyces cerevisiae, other yeasts, and a brown rot fungus. Various waste lignocellulose materials such as wheat and barley straw, recycled wood-fiber based core board, recycled construction waste wood, spruce saw dust, and birch wood were applied to represent wood and non-wood waste lignocellulose of different origin, chemical content and structure. In solid-state single cultivations with the white rot fungus
    P. radiata, both core board and barley straw turned out as suitable substrates for the consolidated bioprocess. Up to 32.4 ± 4.5 g/L of ethanol accumulated in the solid-state core board cultivation
    in 30 days whereas with barley straw, 7.0 ± 0.01 g/L of ethanol was obtained. Similar concentrations of ethanol were produced in increased-volume and higher gravity bioreactor cultivations without chemical, physical or enzymatic pretreatment. In all, our consolidated method adopting a white rot fungus is a promising and economic alternative for second generation bioethanol production from waste and residual lignocelluloses.

    Citation: Hans Mattila, Dina Kačar, Tuulia Mali, Taina Lundell. Lignocellulose bioconversion to ethanol by a fungal single-step consolidated method tested with waste substrates and co-culture experiments[J]. AIMS Energy, 2018, 6(5): 866-879. doi: 10.3934/energy.2018.5.866

    Related Papers:

  • The Polyporales phlebioid white rot fungus Phlebia radiata is efficient in decomposing the wood main components, and in producing ethanol from lignocelluloses and waste materials. Based to these qualifications, the fungus was adopted for design of a consolidated bioprocess method to convert wood waste materials into ethanol without pretreatments. Higher ethanol yield was aimed by introducing collaborative fungal cultivations including isolates of Saccharomyces cerevisiae, other yeasts, and a brown rot fungus. Various waste lignocellulose materials such as wheat and barley straw, recycled wood-fiber based core board, recycled construction waste wood, spruce saw dust, and birch wood were applied to represent wood and non-wood waste lignocellulose of different origin, chemical content and structure. In solid-state single cultivations with the white rot fungus
    P. radiata, both core board and barley straw turned out as suitable substrates for the consolidated bioprocess. Up to 32.4 ± 4.5 g/L of ethanol accumulated in the solid-state core board cultivation
    in 30 days whereas with barley straw, 7.0 ± 0.01 g/L of ethanol was obtained. Similar concentrations of ethanol were produced in increased-volume and higher gravity bioreactor cultivations without chemical, physical or enzymatic pretreatment. In all, our consolidated method adopting a white rot fungus is a promising and economic alternative for second generation bioethanol production from waste and residual lignocelluloses.


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    [1] Niphadkar S, Bagade P, Ahmed S (2018) Bioethanol production: Insight into past, present and future perspectives. Biofuels 9: 229–238. doi: 10.1080/17597269.2017.1334338
    [2] Markandya A, Dhavala K, Palma A (2018) The role of flexible biofuel policies in meeting biofuel mandates. AIMS Energy 6: 530–550. doi: 10.3934/energy.2018.3.530
    [3] Mattila H, Kuuskeri J, Lundell T (2017) Single-step, single-organism bioethanol production and bioconversion of lignocellulose waste materials by phlebioid fungal species. Bioresour Technol 225: 254–261. doi: 10.1016/j.biortech.2016.11.082
    [4] Lundell TK, Mäkelä MR, de Vries RP, et al. (2014) Genomics, lifestyles and future prospects of wood-decay and litter-decomposing Basidiomycota. Adv Bot Res 70: 329–370. doi: 10.1016/B978-0-12-397940-7.00011-2
    [5] Kuuskeri J, Häkkinen M, Laine P, et al. (2016) Time-scale dynamics of proteome and transcriptome of the white-rot fungus Phlebia radiata: growth on spruce wood and decay effect on lignocellulose. Biotechnol Biofuels 9: 192. doi: 10.1186/s13068-016-0608-9
    [6] Kamei I, Hirota Y, Mori T, et al. (2012) Direct ethanol production from cellulosic materials by the hypersaline-tolerant white-rot fungus Phlebia sp. MG-60. Bioresour Technol 112: 137–142. doi: 10.1016/j.biortech.2012.02.109
    [7] Mood SH, Golfeshan AH, Tabatabaei M, et al. (2013) Lignocellulosic biomass to bioethanol, a comprehensive review with a focus on pretreatment. Renew Sust Energ Rev 27: 77–93. doi: 10.1016/j.rser.2013.06.033
    [8] Saha BC, Kennedy GJ, Qureshi N, et al. (2017) Biological pretreatment of corn stover with Phlebia brevispora NRRL-13108 for enhanced enzymatic hydrolysis and efficient ethanol production. Biotechnol Progr 33: 365–374. doi: 10.1002/btpr.2420
    [9] Zabed H, Sahu JN, Suely A, et al. (2017) Bioethanol production from renewable sources: Current perspectives and technological progress. Renew Sust Energ Rev 71: 475–501. doi: 10.1016/j.rser.2016.12.076
    [10] Kuuskeri J, Mäkelä MR, Isotalo J, et al. (2015) Lignocellulose-converting enzyme activity profiles correlate with molecular systematics and phylogeny grouping in the incoherent genus Phlebia (Polyporales, Basidiomycota). BMC Microbiol 15: 217. doi: 10.1186/s12866-015-0538-x
    [11] Mali T, Kuuskeri J, Shah F, et al. (2017) Interactions affect hyphal growth and enzyme profiles in combinations of coniferous wood-decaying fungi of Agaricomycetes. PLoS One 12: 1–21.
    [12] Shah F, Mali T, Lundell TK (2018) Polyporales brown rot species Fomitopsis pinicola: Enzyme activity profiles, oxalic acid production, and Fe3+-reducing metabolite secretion. Appl Environ Microbiol 84: e02662-17.
    [13] Mäkinen MA, Risulainen N, Mattila H, et al. (2018) Transcription of lignocellulose-decomposition associated genes, enzyme activities and production of ethanol upon bioconversion of waste substrate by Phlebia radiata. Appl Microbiol Biotechnol 102: 5657–5672. doi: 10.1007/s00253-018-9045-y
    [14] Pahkala K, Kontturi M, Kallioinen A, et al. (2007) Production of bio-ethanol from barley straw and reed canary grass: A raw material, In 15th European Biomass Conference Exhibition, Berlin, Germany, 7–11.
    [15] Sáez F, Ballesteros M, Ballesteros I, et al. (2013) Enzymatic hydrolysis from carbohydrates of barley straw pretreated by ionic liquids. J Chem Technol Biot 88: 937–941. doi: 10.1002/jctb.3925
    [16] Dien BS (2010) Mass balances and analytical methods for biomass pretreatment experiments. Biomass to Biofuels: Strategies for Global Industries 2010: 213–231.
    [17] Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31: 426–428. doi: 10.1021/ac60147a030
    [18] Kasavi C, Finore I, Lama L, et al. (2012) Evaluation of industrial Saccharomyces cerevisiae strains for ethanol production from biomass. Biomass Bioenerg 45: 230–238. doi: 10.1016/j.biombioe.2012.06.013
    [19] Mohd SA, Abdulla R, Jambo SA, et al. (2017) Yeasts in sustainable bioethanol production: A review. Biochem Biophys Rep 10: 52–61.
    [20] Salvachúa D, Prieto A, López-Abelairas M, et al. (2011) Fungal pretreatment: An alternative in second-generation ethanol from wheat straw. Bioresource Technol 102: 7500–7506. doi: 10.1016/j.biortech.2011.05.027
    [21] Rytioja J, Hildén K, Yuzon J, et al. (2014) Plant-polysaccharide-degrading enzymes from basidiomycetes. Microbiol Mol Biol Rev 78: 614–49. doi: 10.1128/MMBR.00035-14
    [22] Rastogi M, Shrivastava S (2017) Recent advances in second generation bioethanol production: an insight to pretreatment, saccharification and fermentation processes. Renew Sust Energ Rev 80: 330–340. doi: 10.1016/j.rser.2017.05.225
    [23] Arevalo-Gallegos A, Ahmad Z, Asgher M, et al. (2017) Lignocellulose: a sustainable material to produce value-added products with a zero waste approach-A review. Int J Biol Macromol 99: 308–318. doi: 10.1016/j.ijbiomac.2017.02.097
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