Export file:


  • RIS(for EndNote,Reference Manager,ProCite)
  • BibTex
  • Text


  • Citation Only
  • Citation and Abstract

Potential of thin stillage as a low-cost nutrient source for direct cellulose fermentation by Clostridium thermocellum

1 Department of Biosystems Engineering, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada;
2 Department of Microbiology, University of Manitoba, Winnipeg, Manitoba, R3T 2N2, Canada

Special Issues: Advances in Production of Biofuels

Utilization of thin stillage (TS), derived from grain-based ethanol production, was investigated as an alternative source for microbial growth nutrients during direct conversion of cellulose by Clostridium thermocellum DSM 1237. Fermentation end-products synthesized by C. thermocellum grown on media prepared with various concentrations (50-400 g/L) of TS were compared to those synthesized by C. thermocellum grown on reagent grade chemical (reference) medium. Cell-growth in TS media, monitored with the aid of quantitative polymerase chain reactions (qPCR) technique, showed prolonged growth with increasing TS concentration. Final fermentation end-product concentrations from TS media were comparable with those from the reference medium despite lower growth-rates. The volumetric H2 production generated by C. thermocellum grown with medium containing a low concentration (50 g/L) of TS matched the volumetric H2 production by C. thermocellum grown in the reference medium, while higher concentrations (200 g/L) of TS resulted in greater synthesis of ethanol. Supplementation of TS-media with Mg++ enhanced ethanol production, while hydrogen production remained unchanged. These results suggest that TS, an attractive source of low-cost nutrients, is capable of supporting the growth of C. thermocellum and that high concentrations of TS favor synthesis of ethanol over hydrogen from cellulose.
  Article Metrics
Download full text in PDF

Export Citation

Article outline

Copyright © AIMS Press All Rights Reserved