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Human Mesenchymal Cell Attachment, Growth and Biomineralization on Calcium-enriched Titania-polyester Coatings

  • Titanium implant osseointegration can be enhanced by surface modifications that include hydroxyapatite from Ca3(PO4)2. However, CaO may provide more surface calcium (w/w) to induce cellular responses. Therefore, the purpose of this study was to compare responses to novel CaO and Ca3(PO4)2-enriched titania-polyester (PPC) nanocomposite coatings, which were created by an electrostatic ultrafine dry powder coating technique. EDX confirmed the presence of a base polymer scaffold, biocompatible titanium, and CaO or Ca3(PO4)2. SEM showed that human embryonic palatal mesenchymal cells (ATCC CRL-1486) had attached and spread out onto all surfaces within 24 hours. Cell attachment assays showed that there was a progressive increase in cell numbers with surface CaO incorporation (0–5%), such that the PPC + 5% CaO coatings supported the most cells. Furthermore, the PPC + 5% CaO had significantly more (P = 0.006) cells attached to their surfaces than the PPC + 5% CaP coatings and titanium controls, at 24 hours. The PPC + 5% CaO also had more cells that had proliferated on their surfaces over 72 hours, although these differences were not significant (P > 0.05). Similarly, MTT assays showed that the cells had sustained metabolic activity on all surfaces. Again, metabolic activities were highest on the PPC + 5% CaO, and they were significantly higher (P < 0.05) on all CaO-enriched surfaces (1/3/5% CaO) than on the PPC + 5% CaP. Subsequently, Alizarin Red-S staining detected the initiation of biomineralization within 2 weeks, and abundant mineral deposits after 4 weeks of growth on PPC + 5% CaO and PPC + 3% CaO. These nanocomposite coatings have shown that CaO enrichments may provide a heightened cell response when compared to conventional hydroxyapatite.

    Citation: Nicholas Y. Hou, Jesse Zhu, Hiran Perinpanayagam. Human Mesenchymal Cell Attachment, Growth and Biomineralization on Calcium-enriched Titania-polyester Coatings[J]. AIMS Cell and Tissue Engineering, 2017, 1(2): 64-83. doi: 10.3934/celltissue.2017.2.64

    Related Papers:

  • Titanium implant osseointegration can be enhanced by surface modifications that include hydroxyapatite from Ca3(PO4)2. However, CaO may provide more surface calcium (w/w) to induce cellular responses. Therefore, the purpose of this study was to compare responses to novel CaO and Ca3(PO4)2-enriched titania-polyester (PPC) nanocomposite coatings, which were created by an electrostatic ultrafine dry powder coating technique. EDX confirmed the presence of a base polymer scaffold, biocompatible titanium, and CaO or Ca3(PO4)2. SEM showed that human embryonic palatal mesenchymal cells (ATCC CRL-1486) had attached and spread out onto all surfaces within 24 hours. Cell attachment assays showed that there was a progressive increase in cell numbers with surface CaO incorporation (0–5%), such that the PPC + 5% CaO coatings supported the most cells. Furthermore, the PPC + 5% CaO had significantly more (P = 0.006) cells attached to their surfaces than the PPC + 5% CaP coatings and titanium controls, at 24 hours. The PPC + 5% CaO also had more cells that had proliferated on their surfaces over 72 hours, although these differences were not significant (P > 0.05). Similarly, MTT assays showed that the cells had sustained metabolic activity on all surfaces. Again, metabolic activities were highest on the PPC + 5% CaO, and they were significantly higher (P < 0.05) on all CaO-enriched surfaces (1/3/5% CaO) than on the PPC + 5% CaP. Subsequently, Alizarin Red-S staining detected the initiation of biomineralization within 2 weeks, and abundant mineral deposits after 4 weeks of growth on PPC + 5% CaO and PPC + 3% CaO. These nanocomposite coatings have shown that CaO enrichments may provide a heightened cell response when compared to conventional hydroxyapatite.


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    [1] Kim H-W, Kim H-E, Salih V, et al. (2005) Hydroxyapatite and titania sol–gel composite coatings on titanium for hard tissue implants; Mechanical and in vitro biological performance. J Biomed Mater Res, Part B 72B: 1-8. doi: 10.1002/jbm.b.30073
    [2] Ducheyne P, Cuckler JM (1992) Bioactive ceramic prosthetic coatings. Clin Orthop Relat Res 102-114.
    [3] Lee S-H, Kim H-W, Lee E-J, et al. (2006) Hydroxyapatite-TiO2 hybrid coating on Ti implants. J Biomater Appl 20: 195. doi: 10.1177/0885328206050518
    [4] McPherson EJ, Dorr LD, Gruen TA, et al. (1995) Hydroxyapatite-coated proximal ingrowth femoral stems. A matched pair control study. Clin Orthop Relat Res 223-230.
    [5] Ramires PA, Romito A, Cosentino F, et al. (2001) The influence of titania/hydroxyapatite composite coatings on in vitro osteoblasts behaviour. Biomaterials 22: 1467-1474. doi: 10.1016/S0142-9612(00)00269-6
    [6] Gomes PS, Botelho C, Lopes MA, et al. (2010) Evaluation of human osteoblastic cell response to plasma-sprayed silicon-substituted hydroxyapatite coatings over titanium substrates. J Biomed Mater Res B Appl Biomater 94: 337-346.
    [7] Nie X, Leyland A, Matthews A (2000) Deposition of layered bioceramic hydroxyapatite/TiO2 coatings on titanium alloys using a hybrid technique of micro-arc oxidation and electrophoresis. Surf Coat Technol 125: 407-414. doi: 10.1016/S0257-8972(99)00612-X
    [8] Kasuga T, Nogami M, Niinomi M, et al. (2003) Bioactive calcium phosphate invert glass-ceramic coating on β-type Ti–29Nb–13Ta–4.6Zr alloy. Biomaterials 24: 283-290. doi: 10.1016/S0142-9612(02)00316-2
    [9] Protivínský J, Appleford M, Strnad J, et al. (2007) Effect of chemically modified titanium surfaces on protein adsorption and osteoblast precursor cell behavior. Int J Oral Maxillofac Implants 22: 542-550.
    [10] De Groot K, Geesink R, Klein CPAT, et al. (1987) Plasma sprayed coatings of hydroxylapatite. J Biomed Mater Res 21: 1375-1381. doi: 10.1002/jbm.820211203
    [11] Vercaigne S, Wolke JGC, Naert I, et al. (1998) Histomorphometrical and mechanical evaluation of titanium plasma-spray-coated implants placed in the cortical bone of goats. J Biomed Mater Res 41: 41-48. doi: 10.1002/(SICI)1097-4636(199807)41:1<41::AID-JBM5>3.0.CO;2-Q
    [12] Overgaard S, Søballe K, Josephsen K, et al. (1996) Role of different loading conditions on resorption of hydroxyapatite coating evaluated by histomorphometric and stereological methods. J Orthop Res 14: 888-894. doi: 10.1002/jor.1100140607
    [13] Van Dijk K, Schaeken HG, Wolke JCG, et al. (1995) Influence of discharge power level on the properties of hydroxyapatite films deposited on Ti6A14V with RF magnetron sputtering. J Biomed Mater Res 29: 269-276. doi: 10.1002/jbm.820290218
    [14] Yoshinari M, Klinge B, Dérand T (1996) The biocompatibility (cell culture and histologic study) of hydroxy-apatite-coated implants created by ion beam dynamic mixing. Clin Oral Implant Res 7: 96-100.
    [15] Cortecchia E, Pacilli A, Pasquinelli G, et al. (2010) Biocompatible Two-Layer Tantalum/Titania−Polymer Hybrid Coating. Biomacromolecules 11: 2446-2453. doi: 10.1021/bm100619t
    [16] Liu X, Ma PX (2004) Polymeric scaffolds for bone tissue engineering. Ann Biomed Eng 32: 477-486. doi: 10.1023/B:ABME.0000017544.36001.8e
    [17] Ma PX (2008) Biomimetic materials for tissue engineering. Adv Drug Delivery Rev 60: 184-198. doi: 10.1016/j.addr.2007.08.041
    [18] Shi W, Mozumder MS, Zhang H, et al. (2012) MTA-enriched nanocomposite TiO2-polymeric powder coatings support human mesenchymal cell attachment and growth. Biomed Mater 7: 055006. doi: 10.1088/1748-6041/7/5/055006
    [19] Zhang H, Zhu J (2009) Method and apparatus for uniformly dispersing additive particles in fine powders. EP2097161 A1.
    [20] Zhu J, Zhang H (2004) Fluidization additives to fine powders [Internet]. Available from: http://www.google.com/patents?id=SqsQAAAAEBAJ.
    [21] Mozumder MS, Zhu J, Perinpanayagam H (2011) Nano-TiO 2 Enriched Polymeric Powder Coatings Support Human Mesenchymal Cell Attachment and Growth. J Biomater Appl 26: 173-193. doi: 10.1177/0885328210363312
    [22] Mozumder MS, Zhu J, Perinpanayagam H (2011) TiO2-enriched polymeric powder coatings support human mesenchymal cell spreading and osteogenic differentiation. Biomed Mater 6: 035009. doi: 10.1088/1748-6041/6/3/035009
    [23] Mozumder MS, Zhu J, Perinpanayagam H (2012) Titania-polymeric powder coatings with nano-topography support enhanced human mesenchymal cell responses. J Biomed Mater Res, Part A 100A: 2695-2709. doi: 10.1002/jbm.a.34199
    [24] Parirokh M, Torabinejad M (2010) Mineral Trioxide Aggregate: A Comprehensive Literature Review-Part I: Chemical, Physical, and Antibacterial Properties. J Endod 36: 16-27. doi: 10.1016/j.joen.2009.09.006
    [25] Hou NY, Zhu J, Zhang H, et al. (2014) Ultrafine calcium–titania–polyester dry powder coatings promote human mesenchymal cell attachment and biomineralization. Surf Coat Technol 251: 177-185. doi: 10.1016/j.surfcoat.2014.04.022
    [26] Anitua E, Prado R, Orive G, et al. (2015) Effects of calcium-modified titanium implant surfaces on platelet activation, clot formation, and osseointegration. J Biomed Mater Res, Part A 103: 969-980. doi: 10.1002/jbm.a.35240
    [27] Mendes VC, Moineddin R, Davies JE (2009) Discrete calcium phosphate nanocrystalline deposition enhances osteoconduction on titanium-based implant surfaces. J Biomed Mater Res,Part A 90: 577-585.
    [28] Choi J-Y, Jung U-W, Kim C-S, et al. (2013) Influence of nanocoated calcium phosphate on two different types of implant surfaces in different bone environment: an animal study. Clin Oral Implants Res 24: 1018-1022.
    [29] Poulos NM, Rodriguez NA, Lee J, et al. (2011) Evaluation of a novel calcium phosphate-coated titanium porous oxide implant surface: a study in rabbits. Int J Oral Maxillofac Implants 26: 731-738.
    [30] Aniket, El-Ghannam A (2011) Electrophoretic deposition of bioactive silica-calcium phosphate nanocomposite on Ti-6Al-4V orthopedic implant. J Biomed Mater Res, Part B, Appl Biomater 99: 369-379.
    [31] Schneider GB, Zaharias R, Seabold D, et al. (2004) Differentiation of preosteoblasts is affected by implant surface microtopographies. J Biomed Mater Res, Part A 69A: 462-468. doi: 10.1002/jbm.a.30016
    [32] Masaki C, Schneider GB, Zaharias R, et al. (2005) Effects of implant surface microtopography on osteoblast gene expression. Clin Oral Implants Res 16: 650-656. doi: 10.1111/j.1600-0501.2005.01170.x
    [33] Ko YJ, Zaharias RS, Seabold DA, et al. (2007) Osteoblast Differentiation Is Enhanced in Rotary Cell Culture Simulated Microgravity Environments. J Prosthodontics 16: 431-438. doi: 10.1111/j.1532-849X.2007.00204.x
    [34] Perinpanayagam H, Al-Rabeah E (2009) Osteoblasts interact with MTA surfaces and express Runx2. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 107: 590-596. doi: 10.1016/j.tripleo.2008.12.005
    [35] Puchtler H, Meloan SN, Terry MS (1969) On the History and Mechanism of Alizarin and Alizarin Red S Stains for Calcium. J Histochem Cytochem 17: 110-124. doi: 10.1177/17.2.110
    [36] Feng B, Chen J, Zhang X (2002) Interaction of calcium and phosphate in apatite coating on titanium with serum albumin. Biomaterials 23: 2499-2507. doi: 10.1016/S0142-9612(01)00384-2
    [37] Feng B, Weng J, Yang BC, et al. (2004) Characterization of titanium surfaces with calcium and phosphate and osteoblast adhesion. Biomaterials 25: 3421-3428. doi: 10.1016/j.biomaterials.2003.10.044
    [38] Dos Santos EA, Farina M, Soares GA, et al. (2008) Surface energy of hydroxyapatite and beta-tricalcium phosphate ceramics driving serum protein adsorption and osteoblast adhesion. J Mater Sci Mater Med 19: 2307-2316. doi: 10.1007/s10856-007-3347-4
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