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Single cell adhesion strength assessed with variable-angle total internal reflection fluorescence microscopy

1 Laboratoire de Nanotechnologie et d’Instrumentation Optique, Institut Charles Delaunay, UMR CNRS 6281, Université de Technologie de Troyes, 12 rue Marie Curie, CS 42060, 10 004 Troyes cedex, France
2 NanoBioPhotonics group, Institute for Integrative Biology of the Cell, Université Paris-Saclay, CNRS, CEA, Bâtiment 430, 91405 Orsay cedex, France
3 Laboratoire Matrice Extracellulaire et Dynamique Cellulaire, UMR CNRS 7369, Universit´e de Reims Champagne-Ardennes, 51 rue Cognacq-Jay, 51096 Reims Cedex, France

Special Issue: Single Cell analysis

We propose a new strategy to evaluate adhesion strength at the single cell level. This approach involves variable-angle total internal reflection fluorescence microscopy to monitor in real time the topography of cell membranes, i.e. a map of the membrane/substrate separation distance. According to the Boltzmann distribution, both potential energy profile and dissociation energy related to the interactions between the cell membrane and the substrate were determined from the membrane topography. We have highlighted on glass substrates coated with poly-L-lysine and fibronectin, that the dissociation energy is a reliable parameter to quantify the adhesion strength of MDA-MB-231 motile cells.
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Copyright Info: © 2017, Rodolphe Jaffol, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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