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Identification and characterization of Lactococcus starter strains in milk-based traditional fermented products in the region of Iran

Department of Microbiology, Faculty of Science, Qom Branch, Islamic Azad University, Qom, IR Iran

The aim of the present research was identification and investigation of technological attributes of Lactococcus starter strains from traditional dairy products collected from the countryside of Boroujerd in Iran. 33 samples were cultured on selective media M17 and typical colonies surveyed for morphological properties. Totally, 37 strains were isolated based on the diversity in cell morphology and identified using API galleries and carbohydrate fermentation includes 17 strains of Lactococcus lactis (45.96%), 12 strains of Lactococcus garvieae (32.43%) and 8 strains of Lactococcusplantarum (21.62%). Strains were appraised for hydrolysis of L-arginine, casein and starch. Furthermore, strains were evaluated for the ability to grow at temperature 10 °C, 45 °C and presence of 4% and 6.5% NaCl, antibiotic sensitivity, acidification ability, proteolytic and lipolytic activities. Generally, 3 strains of Lc.garvieae (GYLC1, BWLC1, DCLC1) and 7 strains of Lc. lactis (GCLC4, GWLC2, GWLC3, SWLC1, SWLC3, BCLC5, DYLC1) exposed the highest levels of technological properties in order to use as starter cultures.
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1. Mannion CA, Graydonald K, Koski KG (2006) Association of low intake of milk and vitamin D during pregnancy with decreased birth weight. Can Med Assoc J 174: 1273–1277.    

2. Rodriguez E, Gonzalez B, Gaya P, et al. (2000) Diversity of bacteriocins produced by lactic acid bacteria isolated from raw milk. Int Dairy J 10: 7–15.    

3. Rahmati F (2017) Characterization of Lactobacillus, Bacillus and Saccharomyces isolated from Iranian traditional dairy products for potential sources of starter cultures. AIMS Microbiol 3: 815–825.    

4. Abdi R, Sheikhzeinoddin M, Soleimanianzad S (2006) Identification of lactic acid bacteria isolated from traditional Iranian lighvan cheese. Pak J Biol Sci 9: 161–198.

5. Harrigan W, McCance M, (1976) Biochemical test for bacteria, Laboratory methods in food and dairy microbiology,2Eds.,London: Academic Press Inc, 15–22.

6. Anderson NL, Noble MA, Weissfeld AS, et al. (2005) Quality systems in the clinical microbiology laboratory, Washington: American Society for Microbiology, 80–82.

7. De l'antibiogramme de la Société Francaise de Microbiologie (CA-SFM), 2011. Available from: http://docplayer.fr/1350634-Antibiogramme-veterinaire-du-comite-de-l-antibiogramme-de-la-societe-francaise-de-microbiologie.html.

8. Akabanda F, Owusu-Kwarteng J, Tano-Debrah K, et al. (2015) The use of lactic acid bacteria starter culture in the production of Nunu, a spontaneously fermented milk product in Ghana. Int J Food Sci 2014: 721067.

9. Ray PR, Chatterjee K, Chakraborty C, et al. (2013) Lipolysis of milk: A review. IJASVM 1: 58–74.

10. Bergey DH, Buchanan RE, Gibbons NE, (1974) Bergey's manual of determinative bacteriology, New York: Williams & Wilkins, 285–287.

11. Suneel D, Basappa K (2013) Identification and characterization of lactococcus garvieae and antimicrobial activity of its bacteriocin isolated from cow's milk. Asian J Pharm Clin Res 6: 104–108.

12. Smibert R, Krieg NR (1981) General characterization, Manual of methods for general bacteriology, Columbia: ASM, 409–443.

13. Fguiri I, Ziadi M, Atigui M, et al. (2016) Isolation and characterization of lactic acid bacteria strains from raw camel milk for potential use in the production of fermented Tunisian dairy products. Int J Dairy Technol 69: 103–113.    

14. Amor KB, Vaughan EE, de Vos WM (2007) Advanced molecular tools for the identification of lactic acid bacteria. J Nutr 137: 741S–747S.    

15. Balows A, Truper HG, Dworkin M, et al. (1991) The Procaryotes 2nd Edn, New York: Springer-Verlag, 385–389.

16. Kayagil F (1976) Effect of traditional starter cultures on quality of cheese. Middle east technical university; 2006. 88 p.

17. Mangia NP, Murgia MA, Garau G, et al. (2008) Influence of selected lab cultures on the evolution of free amino acids, free fatty acids and Fiore Sardo cheese microflora during the ripening. FoodMicrobiol 25: 366–377.

18. Ayad E, Nashat S, El-Sadek N, et al. (2004) Selection of wild lactic acid bacteria isolated from traditional Egyptian dairy products, according to production and technological criteria.Food Microbiol 21: 715–725.

19. Vuillemard J, Amiot J, Gauthier S (1986) Evaluation de l'activité protéolytique de bactéries lactiques par une méthode de diffusion sur plaque. Microbiol Alim Nutr 3: 327–332.

20. Ma C, Zhang L, Ma D, et al. (2012) Technological characterization of Lactobacilli isolated from Chinese artisanal fermented milks. Int J Dairy Technol 65: 132–139.    

21. Khalid NM, Marth EH (1990) Lactobacilli-their enzymes and role in ripening and spoilage of cheese: A review. J Dairy Sci 73: 2669–2684.    

22. Oterholm A, Ordal ZJ, Witter LD (1968) Glycerol ester hydrolase activity of lactic acid bacteria. ApplMicrobiol 16: 524–527.

23. Kacem M, Zadikaram H, Karam NE (2003) Identification of lactic acid bacteria isolated from milk and fermented olive oil in western Algeria. Actes Inst Agron Vet 23: 2–4.

24. Edalatian MR, Habibi Najafi MB, Mortazavi SA, et al (2013) Isolation and identification of the indigenous lactic acid bacteria from lighvan cheese. Iran J Food tech 9: 9–22.

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