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Electrospun ECM macromolecules as biomimetic scaffold for regenerative medicine: challenges for preserving conformation and bioactivity

1 Politecnico di Milano, Department of Chemistry, Materials and Chemical Engineering “G. Natta”, Via Mancinelli 7, 20131, Milano, Italy
2 INSTM—National Interuniversity Consortium of Materials Science and Technology, Via G. Giusti, 9-50121, Firenze, Italy

Topical Section: Biological and biomimetic materials

The extracellular matrix (ECM), the physiological scaffold for cells in vivo, provides structural support to cells and guaranties tissue integrity. At the same time, however, it represents an extremely complex and finely tuned signaling environment that contributes in regulating tissue homeostasis and repair. ECM can bind, release and activate signaling molecules and also modulate cell reaction to soluble factors. Cell-ECM interactions, as a result, are recognized to be critical for physiological wound healing, and consequently in guiding regeneration. Due to its complexity, mimicking ECM chemistry and architecture appears a straightforward strategy to exploit the benefits of a biologically recognizable and cell-instructive environment. As ECM consists primarily of sub-micrometric fibers, electrospinning, a simple and versatile technique, has attracted the majority efforts aimed at reprocessing of biologically occurring molecules. However, the ability to trigger specific cellular behavior is likely to depend on both the chemical and conformational properties of biological molecules. As a consequence, when ECM macromolecules are electrospun, investigating the effect of processing on their structure, and the extent to which their potential in directing cellular behavior is preserved, appears crucial. In this perspective, this review explores the electrospinning of ECM molecules specifically focusing on the effect of processing on polymer structure and on in vitro or in vivo experiments designed to confirm the maintenance of their instructive role.
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References

1. Flaumenhaft R, Rifkin DB (1991) Extracellular matrix regulation of growth factor and protease activity. Curr Opin Cell Biol 3: 817–823.    

2. Frantz C, Stewart KM, Weaver VM (2010) The extracellular matrix at a glance. J Cell Sci 123: 4195–4200.    

3. Bonnans C, Chou J, Werb Z (2014) Remodelling the extracellular matrix in development and disease. Nat Rev Mol Cell Bio 15: 786–801.

4. Kim SH, Turnbull J, Guimond S (2011) Extracellular matrix and cell signalling: the dynamic cooperation of integrin, proteoglycan and growth factor receptor. J Endocrinol 209: 139–151.    

5. Schultz GS, Wysocki A (2009) Interactions between extracellular matrix and growth factors in wound healing. Wound Repair Regen 17: 153–162.    

6. Roskelley CD, Srebrow A, Bissell MJ (1995) A Hierarchy of ECM-Mediated Signaling Regulates Tissue-Specific Gene-Expression. Curr Opin Cell Bio 7: 736–747.    

7. Altınay S (2016) Is extracellular matrix a castle against to invasion of cancer cells? In: Xu K, Tumor Metastasis, InTech.

8. Laurencin CT, Nair LS (2014) Nanotechnology and regenerative engineering: the scaffold, CRC Press.

9. Smith LA, Ma PX (2004) Nano-fibrous scaffolds for tissue engineering. Colloid Surface B 39: 125–131.    

10. Khajavi R, Abbasipour M (2012) Electrospinning as a versatile method for fabricating coreshell, hollow and porous nanofibers. Sci Iran 19: 2029–2034.    

11. Fessler JH (1974) Self-assembly of collagen. J Supramol Struct 2: 99–102.    

12. Bellingham CM, Keeley FW (2004) Self-ordered polymerization of elastin-based biomaterials. Curr Opin Solid ST M 8: 135–139.    

13. Zhu X, Cui W, Li X, et al. (2008) Electrospun fibrous mats with high porosity as potential scaffolds for skin tissue engineering. Biomacromolecules 9: 1795–1801.    

14. Lee SJ, Yoo JJ, Lim GJ, et al. (2007) In vitro evaluation of electrospun nanofiber scaffolds for vascular graft application. J Biomed Mater Res A 83: 999–1008.

15. Holzwarth JM, Ma PX (2011) Biomimetic nanofibrous scaffolds for bone tissue engineering. Biomaterials 32: 9622–9629.    

16. Xie J, MacEwan MR, Schwartz AG, et al. (2010) Electrospun nanofibers for neural tissue engineering. Nanoscale 2: 35–44.    

17. Li WJ, Tuli R, Okafor C, et al. (2005) A three-dimensional nanofibrous scaffold for cartilage tissue engineering using human mesenchymal stem cells. Biomaterials 26: 599–609.    

18. Mogoşanu GD, Grumezescu AM (2014) Natural and synthetic polymers for wounds and burns dressing. Int J Pharm 463: 127–136.    

19. Sell SA, Wolfe PS, Garg K, et al. (2010) The use of natural polymers in tissue engineering: a focus on electrospun extracellular matrix analogues. Polymers 2: 522–553.    

20. Gomes SR, Rodrigues G, Martins GG, et al. (2015) In vitro and in vivo evaluation of electrospun nanofibers of PCL, chitosan and gelatin: A comparative study. Mater Sci Eng C 46: 348–358.    

21. Herskovits TT, Gadegbeku B, Jaillet H (1970) On the structural stability and solvent denaturation of proteins I. Denaturation by the alcohols and glycols. J Biol Chem 245: 2588–2598.

22. Freedman KJ, Haq SR, Edel JB, et al. (2013) Single molecule unfolding and stretching of protein domains inside a solid-state nanopore by electric field. Sci Rep-UK 3: 1638.    

23. Ingavle GC, Leach JK (2013) Advancements in electrospinning of polymeric nanofibrous scaffolds for tissue engineering. Tissue Eng B 20: 277–293.

24. Tan SH, Inai R, Kotaki M, et al. (2005) Systematic parameter study for ultra-fine fiber fabrication via electrospinning process. Polymer 46: 6128–6134.    

25. Yarin A (2011) Coaxial electrospinning and emulsion electrospinning of core-shell fibers. Polym Advan Technol 22: 310–317.    

26. Xu C, Inai R, Kotaki M, et al. (2004) Aligned biodegradable nanofibrous structure: a potential scaffold for blood vessel engineering. Biomaterials 25: 877–886.    

27. Ding B, Kimura E, Sato T, et al. (2004) Fabrication of blend biodegradable nanofibrous nonwoven mats via multi-jet electrospinning. Polymer 45: 1895–1902.    

28. Del Gaudio C, Bianco A, Grigioni M (2007) Electrospun bioresorbable trileaflet heart valve prosthesis for tissue engineering: in vitro functional assessment of a pulmonary cardiac valve design. Annali dell'Istituto superiore di sanita 44: 178–186.

29. Bhardwaj N, Kundu SC (2010) Electrospinning: a fascinating fiber fabrication technique. Biotechnol Adv 28: 325–347.    

30. Pham QP, Sharma U, Mikos AG (2006) Electrospinning of polymeric nanofibers for tissue engineering applications: a review. Tissue Eng 12: 1197–1211.    

31. Ferreira JL, Gomes S, Henriques C, et al. (2014) Electrospinning polycaprolactone dissolved in glacial acetic acid: Fiber production, nonwoven characterization, and in vitro evaluation. J Appl Polym Sci 131.

32. Gupta RK, Kennel E, Kim KJ (2009) Polymer nanocomposites handbook, CRC press.

33. Patel H, Bonde M, Srinivasan G (2011) Biodegradable polymer scaffold for tissue engineering. Trends Biomater Artif Organs 25: 20–29.

34. Powell S (2010) Langevin Dynamics Study of Water Diffusion in Model Articular Cartilage [Master's Thesis], Queensland University of Technology Brisbane, Brisbane, Australia.

35. Chew SY, Mi R, Hoke A, et al. (2008) The effect of the alignment of electrospun fibrous scaffolds on Schwann cell maturation. Biomaterials 29: 653–661.    

36. Gunatillake PA, Adhikari R (2003) Biodegradable synthetic polymers for tissue engineering. Eur Cells Mater 5: 1–16.

37. Hu X, Liu S, Zhou G, et al. (2014) Electrospinning of polymeric nanofibers for drug delivery applications. J Control Release 185: 12–21.    

38. Bürck J, Heissler S, Geckle U, et al. (2013) Resemblance of electrospun collagen nanofibers to their native structure. Langmuir 29: 1562–1572.    

39. Gugutkov D, Gustavsson J, Ginebra MP, et al. (2013) Fibrinogen nanofibers for guiding endothelial cell behavior. Biomater Sci-UK 1: 1065–1073.    

40. Rnjak J, Li Z, Maitz PK, et al. (2009) Primary human dermal fibroblast interactions with open weave three-dimensional scaffolds prepared from synthetic human elastin. Biomaterials 30: 6469–6477.    

41. Li M, Mondrinos MJ, Gandhi MR, et al. (2005) Electrospun protein fibers as matrices for tissue engineering. Biomaterials 26: 5999–6008.    

42. Khadka DB, Haynie DT (2012) Protein-and peptide-based electrospun nanofibers in medical biomaterials. Nanomed-Nanotechnol 8: 1242–1262.    

43. Malafaya PB, Silva GA, Reis RL (2007) Natural-origin polymers as carriers and scaffolds for biomolecules and cell delivery in tissue engineering applications. Adv Drug Deliver Rev 59: 207–233.    

44. Badylak SF (2005) Regenerative medicine and developmental biology: the role of the extracellular matrix. Anat Rec Part B 287: 36–41.

45. Matthews JA, Wnek GE, Simpson DG, et al. (2002) Electrospinning of collagen nanofibers. Biomacromolecules 3: 232–238.    

46. Matthews JA, Boland ED, Wnek GE, et al. (2003) Electrospinning of collagen type II: a feasibility study. J Bioact Compat Pol 18: 125–134.    

47. Shields KJ, Beckman MJ, Bowlin GL, et al. (2004) Mechanical properties and cellular proliferation of electrospun collagen type II. Tissue Eng 10: 1510–1517.    

48. Jha BS, Ayres CE, Bowman JR, et al. (2011) Electrospun collagen: a tissue engineering scaffold with unique functional properties in a wide variety of applications. J Nanomater 2011: 7.

49. Zeugolis DI, Khew ST, Yew ES, et al. (2008) Electro-spinning of pure collagen nano-fibres-just an expensive way to make gelatin? Biomaterials 29: 2293–2305.    

50. Yang L, Fitie CF, van der Werf KO, et al. (2008) Mechanical properties of single electrospun collagen type I fibers. Biomaterials 29: 955–962.    

51. Lannutti J, Reneker D, Ma T, et al. (2007) Electrospinning for tissue engineering scaffolds. Mater Sci Eng C 27: 504–509.    

52. Liu T, Teng WK, Chan BP, et al. (2010) Photochemical crosslinked electrospun collagen nanofibers: synthesis, characterization and neural stem cell interactions. J Biomed Mater Res A 95: 276–282.

53. Jiang Q, Reddy N, Zhang S, et al. (2013) Water‐stable electrospun collagen fibers from a non‐toxic solvent and crosslinking system. J Biomed Mater Res A 101: 1237-1247.

54. Kazanci M (2014) Solvent and temperature effects on folding of electrospun collagen nanofibers. Mater Lett 130: 223–226.    

55. Fiorani A, Gualandi C, Panseri S, et al. (2014) Comparative performance of collagen nanofibers electrospun from different solvents and stabilized by different crosslinkers. J Mater Sci-Mater M 25: 2313–2321.    

56. Dong Z, Wu Y, Clark RL (2011) Thermodynamic modeling and investigation of the formation of electrospun collagen fibers. Langmuir 27: 12417–12422.    

57. Meimandi-Parizi A, Oryan A, Moshiri A (2013) Role of tissue engineered collagen based tridimensional implant on the healing response of the experimentally induced large Achilles tendon defect model in rabbits: a long term study with high clinical relevance. J Biomed Sci 20: 28.    

58. Rho KS, Jeong L, Lee G, et al. (2006) Electrospinning of collagen nanofibers: effects on the behavior of normal human keratinocytes and early-stage wound healing. Biomaterials 27: 1452–1461.    

59. Liu T, Houle JD, Xu J, et al. (2012) Nanofibrous collagen nerve conduits for spinal cord repair. Tissue Eng A 18: 1057–1066.    

60. Kidoaki S, Kwon IK, Matsuda T (2005) Mesoscopic spatial designs of nano-and microfiber meshes for tissue-engineering matrix and scaffold based on newly devised multilayering and mixing electrospinning techniques. Biomaterials 26: 37–46.    

61. Telemeco T, Ayres C, Bowlin G, et al. (2005) Regulation of cellular infiltration into tissue engineering scaffolds composed of submicron diameter fibrils produced by electrospinning. Acta Biomater 1: 377–385.    

62. Zhong SP, Teo WE, Zhu X, et al. (2007) Development of a novel collagen–GAG nanofibrous scaffold via electrospinning. Mater Sci Eng C 27: 262–266.    

63. Casper CL, Yang W, Farach-Carson MC, et al. (2007) Coating electrospun collagen and gelatin fibers with perlecan domain I for increased growth factor binding. Biomacromolecules 8: 1116–1123.    

64. Barnes CP, Pemble IV CW, Brand DD, et al. (2007) Cross-linking electrospun type II collagen tissue engineering scaffolds with carbodiimide in ethanol. Tissue Eng 13: 1593–1605.    

65. Foltran I, Foresti E, Parma B, et al. (2008) Novel biologically inspired collagen nanofibers reconstituted by electrospinning method. Macromol Symp 269: 111–118.    

66. Dong B, Arnoult O, Smith ME, et al. (2009) Electrospinning of collagen nanofiber scaffolds from benign solvents. Macromol Rapid Comm 30: 539–542.    

67. Timnak A, Gharebaghi FY, Shariati RP, et al. (2011) Fabrication of nano-structured electrospun collagen scaffold intended for nerve tissue engineering. J Mater Sci-Mater M 22: 1555–1567.    

68. Wang Y, Yao M, Zhou J, et al. (2011) The promotion of neural progenitor cells proliferation by aligned and randomly oriented collagen nanofibers through β1 integrin/MAPK signaling pathway. Biomaterials 32: 6737–6744.    

69. Meng L, Arnoult O, Smith M, et al. (2012) Electrospinning of in situ crosslinked collagen nanofibers. J Mater Chem 22: 19412–19417.    

70. Gorgieva S, Kokol V (2011) Collagen-vs. gelatine-based biomaterials and their biocompatibility: review and perspectives, INTECH open access publisher Croatia.

71. Heydarkhan-Hagvall S, Schenke-Layland K, Dhanasopon AP, et al. (2008) Three-dimensional electrospun ECM-based hybrid scaffolds for cardiovascular tissue engineering. Biomaterials 29: 2907–2914.

72. Ratanavaraporn J, Rangkupan R, Jeeratawatchai H, et al. (2010) Influences of physical and chemical crosslinking techniques on electrospun type A and B gelatin fiber mats. Int J Biol Macromol 47: 431–438.

73. Masutani EM, Kinoshita CK, Tanaka TT, et al. (2014) Increasing thermal stability of gelatin by UV-induced cross-linking with glucose. Int J Biomater 2014.

74. Zhang Y, Venugopal J, Huang ZM, et al. (2006) Crosslinking of the electrospun gelatin nanofibers. Polymer 47: 2911–2917.    

75. Panzavolta S, Gioffrè M, Focarete ML, et al. (2011) Electrospun gelatin nanofibers: optimization of genipin cross-linking to preserve fiber morphology after exposure to water. Acta Biomater 7: 1702–1709.    

76. Sisson K, Zhang C, Farach-Carson MC, et al. (2009) Evaluation of cross-linking methods for electrospun gelatin on cell growth and viability. Biomacromolecules 10: 1675–1680.    

77. Madaghiele M, Piccinno A, Saponaro M, et al. (2009) Collagen-and gelatine-based films sealing vascular prostheses: evaluation of the degree of crosslinking for optimal blood impermeability. J Mater Sci-Mater M 20: 1979–1989.    

78. Ki CS, Baek DH, Gang KD, et al. (2005) Characterization of gelatin nanofiber prepared from gelatin–formic acid solution. Polymer 46: 5094–5102.    

79. Powell H, Boyce S (2008) Fiber density of electrospun gelatin scaffolds regulates morphogenesis of dermal–epidermal skin substitutes. J Biomed Mater Res A 84: 1078–1086.

80. Okutan N, Terzi P, Altay F (2014) Affecting parameters on electrospinning process and characterization of electrospun gelatin nanofibers. Food Hydrocolloid 39: 19–26.    

81. Yao R, He J, Meng G, et al. (2016) Electrospun PCL/Gelatin composite fibrous scaffolds: mechanical properties and cellular responses. J Biomat Sci-Polym E 27: 824–838.    

82. Ghasemi-Mobarakeh L, Prabhakaran MP, Morshed M, et al. (2008) Electrospun poly (ɛ-caprolactone)/gelatin nanofibrous scaffolds for nerve tissue engineering. Biomaterials 29: 4532–4539.

83. Dubský M, Kubinová Š, Širc J, et al. (2012) Nanofibers prepared by needleless electrospinning technology as scaffolds for wound healing. J Mater Sci-Mater M 23: 931–941.    

84. Huang ZM, Zhang Y, Ramakrishna S, et al. (2004) Electrospinning and mechanical characterization of gelatin nanofibers. Polymer 45: 5361–5368.    

85. Zhang Y, Ouyang H, Lim CT, et al. (2005) Electrospinning of gelatin fibers and gelatin/PCL composite fibrous scaffolds. J Biomed Mater Res B 72: 156–165.

86. Choktaweesap N, Arayanarakul K, Aht-Ong D, et al. (2007) Electrospun gelatin fibers: effect of solvent system on morphology and fiber diameters. Polym J 39: 622.

87. Song JH, Kim HE, Kim HW (2008) Production of electrospun gelatin nanofiber by water-based co-solvent approach. J Mater Sci-Mater M 19: 95–102.    

88. Zhang S, Huang Y, Yang X, et al. (2009) Gelatin nanofibrous membrane fabricated by electrospinning of aqueous gelatin solution for guided tissue regeneration. J Biomed Mater Res A 90: 671–679.

89. Chen HC, Jao WC, Yang MC (2009) Characterization of gelatin nanofibers electrospun using ethanol/formic acid/water as a solvent. Polym Advan Technol 20: 98–103.

90. Sisson K, Zhang C, Farach‐Carson MC, et al. (2010) Fiber diameters control osteoblastic cell migration and differentiation in electrospun gelatin. J Biomed Mater Res A 94: 1312-1320.

91. Martin SL, Vrhovski B, Weiss AS (1995) Total synthesis and expression in Escherichia coli of a gene encoding human tropoelastin. Gene 154: 159–166.    

92. Yeo GC, Aghaei-Ghareh-Bolagh B, Brackenreg EP, et al. (2015) Fabricated Elastin. Adv Healthc Mater 4: 2530–2556.

93. Nivison‐Smith L, Weiss AS (2012) Alignment of human vascular smooth muscle cells on parallel electrospun synthetic elastin fibers. J Biomed Mater Res A 100: 155-161.

94. Nivison-Smith L, Rnjak J, Weiss AS (2010) Synthetic human elastin microfibers: stable cross-linked tropoelastin and cell interactive constructs for tissue engineering applications. Acta Biomater 6: 354–359.    

95. Rnjak-Kovacina J, Wise SG, Li Z, et al. (2011) Tailoring the porosity and pore size of electrospun synthetic human elastin scaffolds for dermal tissue engineering. Biomaterials 32: 6729–6736.    

96. Boland ED, Matthews JA, Pawlowski KJ, et al. (2004) Electrospinning collagen and elastin: preliminary vascular tissue engineering. Front Biosci 9: e32.    

97. McKenna KA, Hinds MT, Sarao RC, et al. (2012) Mechanical property characterization of electrospun recombinant human tropoelastin for vascular graft biomaterials. Acta Biomater 8: 225–233.    

98. McKenna KA, Gregory KW, Sarao RC, et al. (2012) Structural and cellular characterization of electrospun recombinant human tropoelastin biomaterials. J Biomater Appl 27: 219–230.    

99. Lee KY, Jeong L, Kang YO, et al. (2009) Electrospinning of polysaccharides for regenerative medicine. Adv Drug Deliver Rev 61: 1020–1032.    

100. Li J, He A, Han CC, et al. (2006) Electrospinning of hyaluronic acid (HA) and HA/gelatin blends. Macromol Rapid Comm 27: 114–120.    

101. Schiffman JD, Schauer CL (2008) A review: electrospinning of biopolymer nanofibers and their applications. Polym Rev 48: 317–352.    

102. Um IC, Fang D, Hsiao BS, et al. (2004) Electro-spinning and electro-blowing of hyaluronic acid. Biomacromolecules 5: 1428–1436.    

103. Brenner EK, Schiffman JD, Toth LJ, et al. (2013) Phosphate salts facilitate the electrospinning of hyaluronic acid fiber mats. J Mater Sci 48: 7805–7811.

104. Brenner EK, Schiffman JD, Thompson EA, et al. (2012) Electrospinning of hyaluronic acid nanofibers from aqueous ammonium solutions. Carbohyd Polym 87: 926–929.    

105. Liu Y, Ma G, Fang D, et al. (2011) Effects of solution properties and electric field on the electrospinning of hyaluronic acid. Carbohyd Polym 83: 1011–1015.    

106. Xu S, Li J, He A, et al. (2009) Chemical crosslinking and biophysical properties of electrospun hyaluronic acid based ultra-thin fibrous membranes. Polymer 50: 3762–3769.    

107. Yao S, Wang X, Liu X, et al. (2013) Effects of ambient relative humidity and solvent properties on the electrospinning of pure hyaluronic acid nanofibers. J Nanosci Nanotechno 13: 4752–4758.    

108. Wang X, Um IC, Fang D, et al. (2005) Formation of water-resistant hyaluronic acid nanofibers by blowing-assisted electro-spinning and non-toxic post treatments. Polymer 46: 4853–4867.    

109. Hsu FY, Hung YS, Liou HM, et al. (2010) Electrospun hyaluronate–collagen nanofibrous matrix and the effects of varying the concentration of hyaluronate on the characteristics of foreskin fibroblast cells. Acta Biomater 6: 2140–2147.    

110. Uppal R, Ramaswamy GN, Arnold C, et al. (2011) Hyaluronic acid nanofiber wound dressing-production, characterization, and in vivo behavior. J Biomed Mater Res B 97: 20–29.

111. Ahmed Z, Underwood S, Brown R (2000) Low concentrations of fibrinogen increase cell migration speed on fibronectin/fibrinogen composite cables. Cytoskeleton 46: 6–16.    

112. Ye Q, Zünd G, Benedikt P, et al. (2000) Fibrin gel as a three dimensional matrix in cardiovascular tissue engineering. Eur J Cardio-Thorac 17: 587–591.    

113. Sell SA, Francis MP, Garg K, et al. (2008) Cross-linking methods of electrospun fibrinogen scaffolds for tissue engineering applications. Biomed Mater 3: 045001.    

114. Wnek GE, Carr ME, Simpson DG, et al. (2003) Electrospinning of nanofiber fibrinogen structures. Nano Lett 3: 213–216.

115. Sell S, Barnes C, Simpson D, et al. (2008) Scaffold permeability as a means to determine fiber diameter and pore size of electrospun fibrinogen. J Biomed Mater Res A 85: 115–126.

116. Carlisle CR, Coulais C, Namboothiry M, et al. (2009) The mechanical properties of individual, electrospun fibrinogen fibers. Biomaterials 30: 1205–1213.    

117. McManus M, Boland E, Sell S, et al. (2007) Electrospun nanofibre fibrinogen for urinary tract tissue reconstruction. Biomed Mater 2: 257.    

118. McManus MC, Boland ED, Simpson DG, et al. (2007) Electrospun fibrinogen: feasibility as a tissue engineering scaffold in a rat cell culture model. J Biomed Mater Res A 81: 299–309.

119. Guadiz G, Sporn LA, Simpson-Haidaris PJ (1997) Thrombin cleavage-independent deposition of fibrinogen in extracellular matrices. Blood 90: 2644–2653.

120. Stitzel J, Liu J, Lee SJ, et al. (2006) Controlled fabrication of a biological vascular substitute. Biomaterials 27: 1088–1094.    

121. Schnell E, Klinkhammer K, Balzer S, et al. (2007) Guidance of glial cell migration and axonal growth on electrospun nanofibers of poly-ε-caprolactone and a collagen/poly-ε-caprolactone blend. Biomaterials 28: 3012–3025.    

122. López-Calzada G, Hernandez-Martínez AR, Cruz-Soto M, et al. (2016) Development of meniscus substitutes using a mixture of biocompatible polymers and extra cellular matrix components by electrospinning. Mater Sci Eng C 61: 893–905.

123. Koh HS, Yong T, Chan CK, et al. (2008) Enhancement of neurite outgrowth using nano-structured scaffolds coupled with laminin. Biomaterials 29: 3574–3582.    

124. Zhu Y, Leong MF, Ong WF, et al. (2007) Esophageal epithelium regeneration on fibronectin grafted poly(L-lactide-co-caprolactone) (PLLC) nanofiber scaffold. Biomaterials 28: 861–868.    

125. Cheng Y, Ramos D, Lee P, et al. (2014) Collagen functionalized bioactive nanofiber matrices for osteogenic differentiation of mesenchymal stem cells: bone tissue engineering. J Biomed Nanotechnol 10: 287–298.    

126. Yeo IS, Oh JE, Jeong L, et al. (2008) Collagen-based biomimetic nanofibrous scaffolds: preparation and characterization of collagen/silk fibroin bicomponent nanofibrous structures. Biomacromolecules 9: 1106–1116.    

127. Dhandayuthapani B, Krishnan UM, Sethuraman S (2010) Fabrication and characterization of chitosan–gelatin blend nanofibers for skin tissue engineering. J Biomed Mater Res B 94: 264–272.

128. Altman GH, Diaz F, Jakuba C, et al. (2003) Silk-based biomaterials. Biomaterials 24: 401–416.    

129. Plowman JE, Deb-Choudhury S, Dyer JM (2013) Fibrous protein nanofibers, In: Gerrard JA, Protein Nanotechnology: Protocols, Instrumentation, and Applications, 2nd Eds, Humana Press, 61–76.

130. Kim IY, Seo SJ, Moon HS, et al. (2008) Chitosan and its derivatives for tissue engineering applications. Biotechnol Adv 26: 1–21.    

131. Wen X, Wang Y, Guo Z, et al. (2014) Cauda Equina-Derived Extracellular Matrix for Fabrication of Nanostructured Hybrid Scaffolds Applied to Neural Tissue Engineering. Tissue Eng A 21: 1095–1105.

132. McManus MC, Boland ED, Koo HP, et al. (2006) Mechanical properties of electrospun fibrinogen structures. Acta Biomater 2: 19–28.    

133. Baker S, Sigley J, Helms CC, et al. (2012) The mechanical properties of dry, electrospun fibrinogen fibers. Mater Sci Eng C 32: 215–221.    

134. Wan C, Frydrych M, Chen B (2011) Strong and bioactive gelatin–graphene oxide nanocomposites. Soft Matter 7: 6159–6166.    

135. Nadeem D, Kiamehr M, Yang X, et al. (2013) Fabrication and in vitro evaluation of a sponge-like bioactive-glass/gelatin composite scaffold for bone tissue engineering. Mater Sci Eng C 33: 2669–2678.    

136. Merkle VM, Zeng L, Slepian MJ, et al. (2014) Core‐shell nanofibers: Integrating the bioactivity of gelatin and the mechanical property of polyvinyl alcohol. Biopolymers 101: 336–346.    

137. Hersel U, Dahmen C, Kessler H (2003) RGD modified polymers: biomaterials for stimulated cell adhesion and beyond. Biomaterials 24: 4385–4415.    

138. Paletta JRJ, Bockelmann S, Walz A, et al. (2010) RGD-functionalisation of PLLA nanofibers by surface coupling using plasma treatment: influence on stem cell differentiation. J Mater Sci-Mater M 21: 1363–1369.    

139. Nomizu M, Utani A, Shiraishi N, et al. (1992) The all-D-configuration segment containing the IKVAV sequence of laminin A chain has similar activities to the all-L-peptide in vitro and in vivo. J Biol Chem 267: 14118–14121.

140. Lodish H, Zipursky SL (2001) Molecular cell biology. Biochem Mol Biol Edu 29: 126–133.

141. Peh P, Lim NSJ, Blocki A, et al. (2015) Simultaneous delivery of highly diverse bioactive compounds from blend electrospun fibers for skin wound healing. Bioconjugate Chem 26: 1348–1358.    

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