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Bio-succinic acid production: Escherichia coli strains design from genome-scale perspectives

1 Department of Microbiology & Biotechnology, Federal University Dutse, PMB 7156, Dutse, Jigawa State, Nigeria
2 Faculty of Biosciences & Medical Engineering, Universiti Teknologi Malaysia, 81310 Skudai Johor Bahru, Malaysia

Escherichia coli (E. coli) has been established to be a native producer of succinic acid (a platform chemical with different applications) via mixed acid fermentation reactions. Genome-scale metabolic models (GEMs) of E. coli have been published with capabilities of predicting strain design strategies for the production of bio-based succinic acid. Proof-of-principle strains are fundamentally constructed as a starting point for systems strategies for industrial strains development. Here, we review for the first time, the use of E. coli GEMs for construction of proof-of-principles strains for increasing succinic acid production. Specific case studies, where E. coli proof-of-principle strains were constructed for increasing bio-based succinic acid production from glucose and glycerol carbon sources have been highlighted. In addition, a propose systems strategies for industrial strain development that could be applicable for future microbial succinic acid production guided by GEMs have been presented.
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Copyright Info: © 2017, Bashir Sajo Mienda, et al., licensee AIMS Press. This is an open access article distributed under the terms of the Creative Commons Attribution Licese (http://creativecommons.org/licenses/by/4.0)

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